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61.
Alteration of a membrane associated marker in transplantable rat pancreatic acinar cell carcinoma 总被引:1,自引:0,他引:1
A monoclonal murine IgG has been developed which detects a cellularand intracytoplasmic membrane marker expressed only in the ratpancreatic acinar cell. Membrane aberrations in chemically inducedtransplantable rat pancreas acinar cell adenoma and carcinomawere examined using this IgG. The presence of this membranemarker was assessed by indirect immunofluorescence using paraffinsections of normal pancreas, acinar cell adenoma and transplantedtumors from rats and nude mice. The intracytoplasmic membraneof cells in acinar cell adenoma revealed a much higher intensityof fluorescence compared with normal acinar cells. In contrastthere was an almost complete absence of fluorescent stainingof the intracytoplasmic membranes of acinar cell carcinoma whiletheir cell membranes showed an intensity of fluorescence farexceeding that of normal acinar cells. These findings suggesta defect in the dynamics of membrane-associated antigen(s) inthese cancer cells. 相似文献
62.
Sickle cell disease: imaging of cerebrovascular complications 总被引:3,自引:0,他引:3
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Peripheral blood lymphocytes with known HLA-SD were typed for LD determinants using a panel of primed cells prepared from SD identical lymphocytes. Then these lymphocytes (with known SD and LD) were primed by x-irradiated cells from the same SD and LD group and their secondary reactions were evaluated. Disparity in SD antigens of responder and stimulator cells did not produce primed cells and lymphocytes were primed only for LD differences. This finding allows LD typing using primed cells from a panel of randomly selected lymphocytes regardless of their HLA-SD. 相似文献
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Parsa CF Goldberg MF Hunter DG 《Ophthalmology》2003,110(2):251; author reply 251-251; author reply 252
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Parsa AT Miller JI Eggers AE Ogden AT Anderson RC Bruce JN 《Journal of neuro-oncology》2003,64(1-2):77-87
Summary
Objectives: Adjuvant-linked vaccines have been shown to induce anti-tumor immunity in patients with a variety of solid tumors. In this
study we describe anin vitro model of active immunotherapy using autologous fibroblasts as immunogen. Correlative results from glioma patients immunized
with autologous fibroblasts are also described.
Methods: Peripheral blood lymphocytes (PBLs) from normal subjects were immunizedin vitro against autologous skin fibroblasts coupled to the adjuvant muramyl dipeptide. The lymphocytes developed cell-mediated cytotoxicity
that was measured with a short-term chromium release assay. Results ofin vitro experiments were compared to data derived from glioma patients immunized with subcutaneous injection of an autologous adjuvantlinked
fibroblast vaccine. Glioma target cells and fibroblast immunogens were derived from early passage primary tissue culture.
Results: A comparison of autologous vs. homologous immunogen indicated that major histocompatibility complex matching was required
at the sensitization stage of immunity (17.2±3.4% specific lysis vs. 0.4±3.1%,P<0.01). Pre-treatment of fibroblast immunogen cells with interferon gamma (IFN-γ) was found to significantly increase immunity (42.2±10.0%,P<0.01), as did IFN-γ pre-treatment of tumor target cells (35.8±9.0%,P<0.01). The positive effect of IFN-γ was diminished by treatment of cells with IFN-α. Thesein vitro results correlated well within vivo data derived from glioma patients immunized with an autologous adjuvant-linked fibroblast vaccine. PBLs from patients developed
direct cell-mediated cytotoxicity against autologous tumor cells. Lysis of tumor targets afterin vivo immunization increased over a three-week interval (from 1.2 ± 3.0% to 21.0 ± 3.4%,P < 0.01) while lysis of a non-MHC matched control cell line remained essentially unchanged.
Conclusions: Specific lysis of glioma targetsin vitro was achieved afterin vivo sensitization with autologous adjuvant-linked fibroblasts. Collectively, the data indicate that biochemically modified autologous
cells can stimulate anti-glioma immunity in humans. The degree of specific immunity seen in our patients compares favorably
with other published series using glioma cells as an antigenic source. Accordingly, fibroblasts may represent a practical
alternative to glioma cells for vaccine construction. 相似文献