全文获取类型
收费全文 | 9039篇 |
免费 | 539篇 |
国内免费 | 76篇 |
专业分类
耳鼻咽喉 | 100篇 |
儿科学 | 183篇 |
妇产科学 | 184篇 |
基础医学 | 1061篇 |
口腔科学 | 356篇 |
临床医学 | 622篇 |
内科学 | 2689篇 |
皮肤病学 | 148篇 |
神经病学 | 781篇 |
特种医学 | 290篇 |
外科学 | 1345篇 |
综合类 | 34篇 |
预防医学 | 254篇 |
眼科学 | 95篇 |
药学 | 587篇 |
中国医学 | 27篇 |
肿瘤学 | 898篇 |
出版年
2023年 | 59篇 |
2022年 | 116篇 |
2021年 | 195篇 |
2020年 | 99篇 |
2019年 | 143篇 |
2018年 | 205篇 |
2017年 | 165篇 |
2016年 | 203篇 |
2015年 | 229篇 |
2014年 | 302篇 |
2013年 | 340篇 |
2012年 | 561篇 |
2011年 | 620篇 |
2010年 | 327篇 |
2009年 | 279篇 |
2008年 | 487篇 |
2007年 | 513篇 |
2006年 | 492篇 |
2005年 | 496篇 |
2004年 | 472篇 |
2003年 | 515篇 |
2002年 | 520篇 |
2001年 | 191篇 |
2000年 | 197篇 |
1999年 | 201篇 |
1998年 | 136篇 |
1997年 | 104篇 |
1996年 | 96篇 |
1995年 | 96篇 |
1994年 | 56篇 |
1993年 | 53篇 |
1992年 | 121篇 |
1991年 | 105篇 |
1990年 | 110篇 |
1989年 | 89篇 |
1988年 | 80篇 |
1987年 | 77篇 |
1986年 | 81篇 |
1985年 | 76篇 |
1984年 | 65篇 |
1983年 | 55篇 |
1982年 | 26篇 |
1981年 | 18篇 |
1980年 | 28篇 |
1979年 | 26篇 |
1978年 | 28篇 |
1977年 | 24篇 |
1976年 | 18篇 |
1971年 | 22篇 |
1969年 | 21篇 |
排序方式: 共有9654条查询结果,搜索用时 15 毫秒
71.
Heterotransplantation of gastric adenocarcinoma, induced in dog by N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) has been attempted to nude mouse. Biopsied materials from Borrmann 3 type carcinoma, which showed signet-ring cell carcinoma at the subcardiac region of a beagle dog, were inoculated into the muscles of the hind legs of BALB/c-nu/nu nude mice. Serial transmission was obtained in 2 lives, so far 4-5 passages. The histological findings of the grafts were mainly poorly differentiated adenocarcinomas. 相似文献
72.
Tomomi Nakagawa Kazuyoshi Shigehara Yuki Kato Shohei Kawaguchi Hiroki Nakata Taito Nakano Kouji Izumi Yoshifumi Kadono Atsushi Mizokami 《Journal of medical virology》2023,95(1):e28110
Although urine and bladder washing samples are commonly used for the cytological evaluation of the bladder mucosa, it has been unknown whether these samples are likely suitable to investigate human papillomavirus (HPV) prevalence in the urinary bladder. The present study aimed to elucidate the appropriateness of spontaneously voided urine or bladder washing in screening HPV infection in the urinary bladder. Urine and bladder washing samples were obtained from 201 patients who underwent transurethral bladder tumor resection. After extracting DNA from both samples, HPV-DNA was examined using a nested polymerase chain reaction with GP5+/6+ and MY09/11 primers. HPV genotyping was performed in the HPV-positive samples. In situ hybridization (ISH) was performed to observe the HPV-DNA localization in urothelial cells among cytological samples and paraffin-embedded tumor tissues in HPV-positive washing samples. HPV prevalence in urine and washing samples were 9.5% and 7.0%, respectively. High-risk HPV prevalence in urine and washing samples was 7.5% and 4.0%, respectively. The most common HPV type was HPV 16, followed by HPV 52 and HPV 18 in both samples. HPV type distribution in both samples was not in agreement (κ = −0.431). The ISH analysis revealed that HPV-DNA signal was observed in urothelial cells of five (55.7%) of nine detectable HPV-positive cytological samples. Six (66.7%) of nine HPV-positive cases had HPV-DNA signals in tumor tissue. The use of washing samples was likely applicable for investigating HPV prevalence in the urinary bladder. HPV-DNA detected in washing samples might be frequently derived from the urinary bladder. 相似文献
73.
Shun Kaneko Masayuki Kurosaki Toshie Mashiba Hiroyuki Marusawa Masahiko Kondo Yuji Kojima Yasushi Uchida Hideki Fujii Takehiro Akahane Hitoshi Yagisawa Atsunori Kusakabe Haruhiko Kobashi Takehiko Abe Hideo Yoshida Chikara Ogawa Koichiro Furuta Nobuharu Tamaki Keiji Tsuji Tomomichi Matsushita Namiki Izumi the Japanese Red Cross Liver Study Group 《Journal of medical virology》2023,95(1):e28210
Nucleos(t)ide analogs (NAs) cannot completely suppress the risk of hepatocellular carcinoma (HCC) in patients with chronic hepatitis B (CHB). This study aimed to identify the risk factors for HCC development in naïve CHB patients treated with current NA. Patients receiving NA (n = 905) were recruited retrospectively from the 17 hospitals of the Japanese Red Cross Liver Study Group. All treatment-naïve patients had been receiving current NA continuously for more than 1 year until the end of the follow-up. We analyzed the accuracy of predictive risk score using the area under receiver operating characteristic curve. The albumin–bilirubin (ALBI) score was significantly improved by NA therapy (−0.171 ± 0.396; p < 0.001 at Week 48). A total of 72 (8.0%) patients developed HCC over a median follow-up of 6.2 (1.03–15.7) years. An independent predictive factor of HCC development was older age, cirrhosis, lower platelet counts at baseline and ALBI score, and alpha-fetoprotein (AFP) at 1 year after NA therapy according to multivariate analysis. The accuracy was assessed using the PAGE-B, mPAGE-B, aMAP, APA-B, and REAL-B scores that included these factors. Discrimination was generally acceptable for these models. aMAP and REAL-B demonstrated high discrimination with 0.866/0.862 and 0.833/0.859 for 3- and 5-year prediction from the status of 1 year after NA therapy, respectively. Baseline age and platelet count, as well as ALBI and AFP one year after NA, were useful for stratifying carcinogenesis risk. The aMAP and REAL-B scores were validated with high accuracy in Japanese CHB patients. 相似文献
74.
Tomoaki Yuhi Akihiko Wada Ryuichi Yamamoto Masanobu Urabe Hiromi Niina Futoshi Izumi Toshihiko Yanagital 《Naunyn-Schmiedeberg's archives of pharmacology》1994,350(2):209-212
We have previously reported that in bovine adrenal chromaffin cells Ptychodiscus brevis toxin-3 (PbTx-3) does not alter the veratridine-induced 22Na influx when given alone, but increases the influx of 22Na when co-applied with either - or -scorpion venom (Wada et al. 1992). In the present study, we characterized [3H]PbTx-3 binding in bovine adrenal chromaffin cells. [3H]PbTx-3 binding was saturable, reversible and of high-affinity with an equilibrium dissociation constant (Kd) of 32.0±4.9 nmol/1 and a maximum binding capacity Bmax of 6.2 ± 1.2 pmol/4 × 106 cells (4.5 ± 0.9 pmol/mg cell protein). A Hill plot revealed the lack of cooperative interaction among the binding sites. Unlabelled PbTx-3 inhibited [3H]PbTx-3 binding with an IC50 of 31 nmol/l. However, tetrodotoxin, veratridine, - and -scorpion venom, or veratridine in combination with either - or -scorpion venom did not alter [3H]PbTx-3 binding. All these results suggest that PbTx-3 binds to a site (site 5) distinct from the previously known four toxin binding sites, which does not gate voltage-dependent Na channels by itself, but is specifically involved in the allosteric modulation of Na channels in adrenal medullary cells.
Correspondence to: A. Wada at the above address 相似文献
75.
The delayed neurotoxic organophosphate, diisopropylfluorophosphate (DFP) binds with high affinity to membrane-bound proteins
from chicken nerve tissues. The autoradiographic distribution of [3H]DFP binding sites in spinal cord sections of chicken showed higher concentrations of binding sites in gray matter than in
white matter. In the cervical region, fairly high densities of [3H]DFP binding sites were found in laminae X and to a lesser extent, in the ventral horn gray matter. To identify the membrane-associated
DFP-binding proteins, detergent-solubilized membranes were labeled widi 5-10nM [3H]DFP (10pmol/mg protein) for 70 min at 37°C. Gel-exclusion chromatography of the [3H]DFP-radiolabeled membranes indicated at least two major radioactive proteins with apparent molecular weights of 150-670
kDa and 40-129 kDa. Although we could not identify the high affinity DFP binding proteins, the autoradiographic experiments
clearly demonstrated that the DFP binding proteins localized on gray matter of chicken spinal cord. 相似文献
76.
77.
Internal mammary artery embolization for hemoptysis 总被引:2,自引:0,他引:2
Hashimoto M Heianna J Okane K Izumi J Watarai J 《Acta radiologica (Stockholm, Sweden : 1987)》1999,40(2):187-190
PURPOSE: The purpose of this study was to determine the factors influencing development of blood supply from the internal mammary artery and to discuss the value of embolization of the abnormal branches from this vessel using small particles following occlusion of the normal distal branches using microcoils in treating hemoptysis. MATERIAL AND METHODS: Five patients with hemoptysis underwent internal mammary artery embolization with coaxial microcatheter systems. Bronchoscopy, chest radiographs, and CT were performed to determine the site and extent of the basic disease before embolotherapy in all patients. RESULTS: In all patients, pulmonary lesions had extended from the lung to the adjacent pleural surface at the anterior lung field. Four patients underwent embolization from the proximal portion of the internal mammary artery following distal coil embolization. One patient who underwent only proximal embolization had recurrent bleeding. CONCLUSION: The internal mammary artery contributes to the perfusion of lesions responsible for hemoptysis when the basic lesion involves the pulmonary parenchyma adjacent to the anterior pleural surface. Initial distal occlusion of the internal mammary artery may improve the efficacy of embolization of this artery for hemoptysis. 相似文献
78.
Izumi Y Inoue H Kawano T Tani M Tada M Okabe S Takeshita K Endo M 《Surgical endoscopy》1999,13(4):358-360
BACKGROUND: We have performed endoscopic mucosal resection of the esophagus (172 cases), stomach (102 cases), and colon (28 cases) using a transparent plastic cap. Because the lesion-bearing mucosa is suctioned up inside the cap under endoscopic suction, the mucosa should be dissected sufficiently from the proper muscle layer to prevent perforation. METHODS: To avert the risk of perforation, we introduced endosonographic assessment of submucosal dissection (47 cases). In all cases, just keeping the ultrasonic probe on the surface of the mucosa allowed us to evaluate whether the mucosal lesion was lifted up sufficiently from the proper muscle layer after local saline injection. RESULTS: It was possible to confirm that the muscle layer was kept outside the strangulating snare by the same procedure (32 of 37 cases, 86.5%). CONCLUSIONS: We experienced five muscular resections in cases without the ultrasonic probe and no muscular resection with the ultrasonic probe. Thus we recommend endosonographic assessment during endoscopic mucosal resection to enhance its safety. 相似文献
79.
Shiomi H Hase T Matsuno S Izumi M Tatsuta T Ito F Kishida A Tani T Kodama M 《Surgery today》1999,29(12):1280-1284
An 18-year-old male was admitted to our Emergency Department with a traumatic abdominal wall hernia (TAWH) of the left lower
quadrant (LLQ) after suffering hypogastric blunt injury and urogenital lacerations in a motorcycle accident. Upright chest
X-ray showed a small amount of right infradiaphragmatic free air, and a computed tomographic (CT) scan demonstrated an abdominal
wall hernia. At surgery, no impairment was found in the digestive tract, and an abdominal herniorrhaphy was performed. It
is suggested that the free air had passed through a connection between the scrotal laceration and the contralateral abdominal
defect via the subcutaneous space and was palpated as emphysema. This is a new type of TAWH, which suggests that blunt abdominal
trauma may result in negative pressure in the subcutaneous and peritoneal cavity, and this could reflect the pathophysiology
of TAWH. 相似文献
80.