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Recent in vitro studies have shown that di-2-ethylhexyl-phthalate (DEHP) inhibits the deterioration of RBCs during refrigerated storage in containers that use this compound as a plasticizer. The experiments described in this report were designed to assess whether this in vitro protective effect of DEHP would result in a prolonged in vivo survival of RBCs infused into normal human recipients. Whole blood collected from ten normal donors was stored for 35 days in citrate-phosphate- dextrose-adenine (CPDA-1) anticoagulant contained in polyvinylchloride (PVC) bags plasticized with DEHP or a trimellitate compound that is known to have low leachability. Aliquots of RBCs from each container were then labeled with chromium-51 and were reinfused into the original donors. For blood stored in DEHP-plasticized PVC bags, 24% more red cells survived in vivo 24 hours after reinfusion than was observed when the blood had been stored in trimellitate-plasticized bags (P less than .001). Whole blood stored in glass bottles showed a similar improvement in in vivo survival when DEHP was added in weekly increments to mimic the accumulation of this plasticizer seen during storage in plastic containers. Survival of packed red cells stored in the presence of DEHP increased by 14% compared with storage in trimellitate-plasticized bags (P less than .05). In agreement with previous studies, hemolysis and microvesicle formation were also reduced in the presence of DEHP. These results suggest that proposed new storage systems lacking DEHP should be carefully evaluated to determine whether adequate post-transfusion survival of RBCs may be achieved. 相似文献
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Osteopenia in children: CT assessment 总被引:1,自引:0,他引:1
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A S Nikonenko B P Sandomirski? A A Kovalev S N Zavgorodni? N A Volkova I V Khodakovskaia 《Klinichna khirurhiia / Ministerstvo okhorony zdorov'ia Ukra?ny, Naukove tovarystvo khirurhiv Ukra?ny》1991,(12):22-24
In the clinic, within the recent 5 years, the surgical treatment of complication of diabetes mellitus were used in 88 patients. Xenotransplantation of islet pancreatic tissue was performed in 70 patients, deportalization of pancreatic blood flow--in 18. Resulting from the operations performed, a pronounced antidiabetic effect (reduction of daily insulin dosage, stabilization of the incidence of complications) was noted in the majority of patients. Occasionally, the staged combined use of both the methods for surgical treatment of complications of diabetes mellitus is possible. 相似文献
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Nikonenko BV Sacksteder KA Hundert S Einck L Nacy CA 《Recent patents on anti-infective drug discovery》2008,3(2):102-116
Tuberculosis (TB) remains one of the leading infectious killers in the world. New anti-TB drugs and more effective drug combinations are urgently needed, particularly given the increasing incidence of drug-resistant TB and HIV-TB co-infection. This review describes the available mouse models of TB and describes their utility in the evaluation of new TB drug candidates and in the evaluation of the efficacy of new TB drug combinations. Some of the most recent patents on promising TB drug-candidates are also mentioned here. 相似文献
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E. Arredondo M. López-Fraga E. Chatzixiros B. Senemaud P. Brezovsky C. Carella C. Ballesté A. Aydin Mehmet G. Tomadze I. Codreanu A.A. Sarkissian M. Simeonova A. Nikonenko V. Zota M.P. Gómez M. Manyalich C. Bolotinha A. Franca K.-H. Buchheit 《Transplantation proceedings》2018,50(2):374-381