SLE小鼠高IgG血症Fcgr2b基因序列分析

目的: 测定系统性红斑狼疮(SLE)小鼠模型-NZB/WF1双亲NZB,NZW小鼠 Fcgr2b基因启动子区核酸序列,明确Fcgr2b基因启动子区的突变性质.方法:扩增NZB,NZW小鼠Fcgr2b基因启动子DNA进行核酸序列分析.采用ELISA法测定、比较(NZB×NZW )F1×NZW回交小鼠Fcgr2b基因B/W型与W/W型组间血清总IgG 水平.结果:NZB小鼠Fcgr2b基因启动子区与正常鼠Balb/C相比存在2个部位碱基缺失,分别为13 bp及3 bp.NZW小鼠除有3个碱基置换外,与Balb/C鼠该基因启动子区序列相同.回交小鼠Fcgr2b基因B/W型组血清总 IgG水平明显高于W/W型组(P<0.0001).结论:NZB小鼠Fcgr2b基因启动子区存在碱基缺失,且该缺失突变可能与血清总IgG水平升高有关.     

儿童孟氏骨折并同侧桡尺骨远端骨骺骨折

儿童孟氏骨折并桡尺骨远端骨骺骨折临床上不多见，其病理改变复杂，治疗较困难，近10年来，在全国名老中医朱少廷教授指导下，采用手法复位、杉树皮夹板外固定治疗15例，收到满意疗效，现报告如下。     

联合检测自发凋亡率及p27kip1在膀胱移行细胞癌中的意义

目的探讨自发凋亡率、p27kip1在人膀胱移行细胞癌中表达的临床意义。方法采用TUNEL法检测自发凋亡细胞,用S’P法检测p27kip1基因在50例膀胱移行细胞癌中的表达,分析各检测指标在上述标本中的表达及与膀胱移行细胞癌分级、临床分期间的关系。结果在膀胱移行细胞癌中,自发凋亡率AI均数为(3.0±1.5)%,p27kip1基因的阳性表达率为58.0%(29/50)。其二者的阳性表达均随膀胱癌分级、分期的升高而降低。p27kip1蛋白阳性组中AI均数显著高于p27kip1蛋白阴性组(P<0.05)。结论p27kip1蛋白和自发凋亡率与肿瘤的恶性程度和进展密切相关,联合检测p27kip1和自发凋亡率有助于更准确地解释和描述膀胱癌的生物学行为。     

Modulation of vocal and nonvocal behavior in adult squirrel monkeys by selective MAO-A and MAO-B inhibition

The acute effects of monoamine oxidase inhibitors L-deprenyl (0.5-5.0 mg/kg), clorgyline (1.0-10.0 mg/kg), and milacemide (100-400 mg/kg) on the behavior of adult male squirrel monkeys were examined during brief social separations beginning 60 min after subcutaneous drug administration. All three drugs selectively reduced the rate of calling during social separation at doses which did not affect time spent in locomotion, nor the frequency of vigilance-checking. Deprenyl and milacemide, but not clorgyline, produced concurrent decreases in locomotion at the higher doses tested. At threshold doses, clorgyline, but not deprenyl or milacemide, increased call duration and decreased call peak frequency compared to vehicle control values. Plasma levels of MHPG were decreased by an optimal dose of clorgyline but not by deprenyl or milacemide, indicating that substrate specificity was maintained at the drug doses employed. We conclude that different MAO substrates mediate different aspects of vocal and nonvocal behavior in adult male squirrel monkeys.     

经尿道膀胱肿瘤切除专用手术刀具的临床应用

目的对经尿道膀胱肿瘤切除专用手术刀具治疗浅表性膀胱肿瘤进行临床应用评价。方法在传统电切刀的远端添加保护襻组成膀胱肿瘤切除专用手术刀具。使用该种手术刀具治疗浅表性膀胱肿瘤48例,使用方法与普通电切刀近似。男26例,女22例。年龄23~76岁,平均43岁。肿瘤分期Tis者1例,T0~T1者47例。肿瘤分级G1~G2者36例,G3~G4者12例。瘤体直径0.5~8.0 cm。肿瘤位于膀胱顶部4例,左侧壁11例,右侧壁9例,前壁4例,后壁13例,颈部7例。肿瘤单发31例,多发17例。术后常规卡介苗膀胱灌注1年,每3个月行膀胱镜检查1次。结果48例平均手术时间(23±10)m in。术中18例(37.5%)发生闭孔神经反射,未出现膀胱穿孔病例;无瘤体后部的正常膀胱黏膜误切。46例术后随访1年,14例复发,均为远位新生膀胱肿瘤,术后3个月时发现1例,4~6个月时3例,7~9个月时6例,10~12个月时4例。结论使用该专用手术刀具治疗浅表性膀胱肿瘤容易控制切除深度,可最大限度地避免膀胱穿孔,并可保护大体积肿瘤后部盲区的正常黏膜以免误切。