β-TrCP1在非霍奇金淋巴瘤细胞黏附耐药中的作用*

目的：探讨β转导重复包含蛋白(β-transducin repeat containing protein 1, β-TrCP1)对非霍奇金淋巴瘤(non-Hodgkin''s lymphoma, NHL)细胞黏附耐药性的影响。方法：采用Western Blot法检测NHL细胞黏附到骨髓基质细胞后β-TrCP1的表达水平,钙黄绿素检测法检测β-TrCP1对NHL细胞黏附率的影响,CCK8、TUNEL检测干扰β-TrCP1后对NHL细胞耐药性的影响。结果：β-TrCP1蛋白在NHL细胞黏附到骨髓基质细胞后表达升高,且干扰β-TrCP1的表达能明显下调NHL细胞的黏附率。将NHL细胞黏附到骨髓基质细胞并加入米托蒽醌后,干扰β-TrCP1的表达能明显抑制NHL细胞活性并增加凋亡细胞数量。结论：干扰β-TrCP1能抑制NHL细胞黏附介导的耐药。     

不同时间针刺足三里穴对炎性大鼠脊髓、背根神经节中TRPV4蛋白表达的影响

目的研究不同时间针刺足三里穴对炎性大鼠脊髓及背根神经节中香草酸瞬时电位受体4(TRPV4)蛋白表达的影响。方法将54只清洁级的雄性SD大鼠驯化7 d后,进行基础痛阈筛选,然后按痛阈结果随机分组为造模组36只、空白组18只,造模组在右足足垫部皮内注射完全弗氏佐剂0.1 mL,空白对照组注射0.1 mL生理盐水。造模成功的大鼠又按照基础痛阈随机分为模型组18只和针刺组18只,每组按照不同的处理时间又分为ZT8(15:00)、ZT16(23:00)2个时间亚组,每组9只。在2个时间点进行处理,针刺组在相应时间点手针针刺患侧足三里30 min,其间每5 min捻转1 min,频率为120次/min,模型组、空白组捆绑固定30 min,不做其他处理,1次/d,共治疗7 d。在第7天治理结束后检测相应时间点大鼠的痛阈,痛阈检测后取材,提取大鼠腰椎4～6的脊髓及背根神经节,采用免疫组化的方法检测大鼠背根神经节以及脊髓中TRPV4的表达。结果①在ZT8时间点:模型组大鼠与空白组相比,背根神经节中TRPV4表达量显著增加(P<0.01);脊髓组织TRPV4蛋白表达量明显上调(P<0.05)。针刺组大鼠与模型组相比,针刺组背根神经节TRPV4表达量显著下降(P<0.01),脊髓组织TRPV4蛋白表达量无明显差异(P>0.05)。②在ZT16时间点:与空白组相比,模型组大鼠背根神经节TRPV4表达量明显增加(P<0.05),脊髓组织TRPV4蛋白表达量明显上调(P<0.01)。与模型组相比,针刺组大鼠背根神经节TRPV4表达量无明显差异(P>0.05),脊髓组织TRPV4蛋白表达量降低(P<0.05)。结论炎性痛能够诱发大鼠脊髓和背根局部神经节TRPV4蛋白表达的上调;针刺足三里穴能够调节炎性痛大鼠脊髓与背根神经节TRPV4的蛋白含量。     

双侧局限性结核性脓胸I期手术临床疗效分析

目的 探讨Ⅰ期双侧局限性结核性脓胸胸膜剥脱术的安全性及临床疗效。方法 回顾性选取2012—2017年间我院收治的符合手术指征的双侧结核性脓胸150例,分为一期手术组（简称“一期组”）和分期手术组（简称“分期组”）各75例,并顺利施行胸膜剥脱术。回顾性分析两组间在术中出血量、术后引流量、左右侧拔管时间、术后住院时间、患者焦虑状况及住院费用方面的差异。分析一期组和分期组患者术前、术后3个月肺功能指标的差异,包括用力肺活量forced vital capacity（FVC）、第一秒用力呼气容积forced expiratory volume in the first second（FEV₁）、最大呼气峰流速peak expiratory flow rate（PEF）、最大通气量maximal voluntary ventilation（MVV）。肺功能测定结果均按“测定值/正常预计值×100%”表示,所获得计量资料以“ x ±s”表示,统计学处理采用t检验,以P<0.05为差异有统计学意义。结果 一期组的术中出血量为（728.2±159.4) mL、术后3 d引流量为（1 312.3±62.7） mL、左右侧拔管时间为（6.4±1.5） d、（6.5±1.5） d,与分期组的（731.3±151.0） mL、（1 293.7±60.5） mL、（6.3±1.5） d、（6.3±1.7） d比较,差异无统计学意义（P>0.05）。一期组的术后哌替啶用量（15.3±1.2) mg、状态焦虑评分（40.5±2.0)、术后住院时间（11.1±1.9) d、手术及术后总费用（24 351.3±1 254.3) 元与分期组为（20.2±1.5) mg、（44.6±2.3)、（17.7±1.3) d、（30 446.2±1 406.9） 元比较, 差异均具有统计学意义（P<0.05）。75例一期手术组中,术前、术后3个月肺功能检测指标比较,包括FVC分别为（71.40±3.70）%、（91.20±4.60）%、FEV₁分别为（63.20±2.40）%、（84.80±2.60）%、PEF分别为（64.00±2.60）%、（72.40±2.70）%、MVV分别为（62.10±3.70）%、（ 84.70±4.70）%,差异均有统计学意义（P<0.05）。结论 双侧局限性结核性脓胸行一期手术,安全有效,一期组患者的术后哌替啶用量、状态焦虑评分、住院时间及费用优于分期组。     

血清ANCA与ASCA和GAB联合检测对炎症性肠病的临床价值

目的 探讨血清抗中性粒细胞胞浆抗体(ANCA)与抗酿酒酵母抗体(ASCA)和抗小肠杯状细胞抗体(GAB)联合检测对炎症性肠病(IBD)的临床价值.方法 选取本院2017年5月至2019年5月收治的IBD患者63例作为观察组,并选取同期健康体检者50名作为对照组,全部受检者均行血清ANCA、ASCA和GAB检测,比较两组检测阳性率及联合指标检测与单项指标检测诊断IBD的结果.结果 观察组血清ANCA阳性率(71.43％)、ASCA阳性率(75.60％)、GAB阳性率(80.95％)均高于对照组(32.00％、26.00％、24.00％),差异有统计学意义(P<0.05);血清ANCA、ASCA、GAB联合检测诊断IBD特异性(100.00％)、敏感性(95.24％)、准确性(97.35％)均高于单项指标ANCA(64.00％、68.25％、66.37％)、ASCA(78.00％、69.84％、73.45％)、GAB(74.00％、79.37％、76.99％),差异有统计学意义(P<0.05).结论 血清ANCA、AS-CA和GAB联合检测诊断IBD临床应用价值较高,值得临床推广运用.     

注射用血塞通治疗脑梗死恢复期瘀血阻滞证的随机对照研究

目的:评价注射用血塞通(冻干)治疗脑梗死恢复期瘀血阻滞证的有效性和安全性。方法:采用区组随机、盲法、阳性药/安慰剂平行对照、多中心临床研究的方法,选取2012年10月至2014年1月北京中医药大学东方医院收治的脑梗死恢复期患者282例,随机分为观察组(高剂量) 105例、阳性药对照组35例、低剂量组71例及安慰剂组71例。疗程均为15d。观察各组患者治疗前后美国国立卫生研究所卒中量表(NIHSS)评分、改良Rankin量表(mRS)评分、Barthel指数、中医证候积分、各项生命体征及相关实验室检查的变化,并观察不良反应。结果:观察组在改善NIHSS评分、mRS评分、Barthel指数及中医证候方面疗效皆优于阳性药物组、低剂量组、安慰剂组,差异有统计学意义(P 0. 05);观察组与其他各组比较,能明显改善中医单项症状中下肢不遂、口舌歪斜、舌强言謇或不语、偏身麻木、头痛、头晕、唇色紫暗(P 0. 05)。各组不良反应发生率差异无统计学意义(P 0. 05)。结论:注射用血塞通(冻干)用于脑梗死恢复期疗效好,安全性较高。     

人参亲环素蛋白原核表达及其抗真菌活性

目的：研究人参亲环素蛋白（pgCyP）体外抗真菌活性。方法采用 RT-PCR 技术,扩增人参 cyclo-philin（pgCyP）基因片段,构建原核表达质粒并转入大肠杆菌中,利用 IPTG 诱导表达目的蛋白,通过亲和纯化获得 pgCyP 蛋白,纸片扩散法验证目的蛋白的抗菌活性。结果人参 CyP 基因全长为522个碱基,编码174个氨基酸;目的蛋白经纯化后,SDS-PAGE 分析显示单一条带;抑菌试验表明该重组蛋白可明显抑制疫霉菌菌丝的生长。结论成功克隆并表达具有抗菌活性的 pgCyP 蛋白,为进一步研究 pgCyP 在人参抗真菌中的作用机制奠定了基础。     

Fabrication and wireless micromanipulation of magnetic-biocompatible microrobots using microencapsulation for microrobotics and microfluidics applications

It is important to fabricate biocompatible and chemical-resistant microstructures that can be powered and controlled without a tether in fluid environment for applications when contamination must be avoided, like cell manipulation, and applications where connecting the power source to the actuator would be cumbersome, like targeted delivery of chemicals. In this work, a novel fabrication method was described to encapsulate magnetic composite into pure SU-8 structures, enabling the truly microscale ferromagnetic microrobots biocompatible and chemical resistant. The microrobots were developed using the simple multilayer photolithography that allows us to mass produce and were actuated contact-free by external magnetic field to complete micromanipulations of micro-objects. The microrobots were actuated moving along a preplanned path to transport a glass microsphere object at an approximately average speed of 1.1?mm/sec and can be operated to rotate, aim at targets and collect objects.     

MiR-19a mediates the negative regulation of the NF-κB pathway in lipopolysaccharide-induced endometritis by targeting TBK1

Objective

In both humans and animals, endometritis is severe inflammation of the uterus, and it causes great economic losses in dairy cow production. MicroRNAs have been reported to play an important role in various inflammatory diseases. However, the regulatory mechanisms of miR-19a in endometritis remain unclear. Thus, the aims of this study are to investigate the role of miR-19a in a mouse model of lipopolysaccharide (LPS)-induced endometritis and elucidate the possible mechanisms in bovine endometrial epithelial cells (bEECs).

Methods and results

Histological analysis showed that LPS induced severe pathological changes, suggesting that the endometritis mouse model was well established. The qPCR assay indicated that miR-19a expression in the uterine tissues of mice with endometritis and in bEECs with LPS stimulation was significantly reduced. The overexpression of miR-19a significantly decreased the expression of inflammatory cytokines (TNF-α, IL-6 and IL-1β) and the phosphorylation of NF-κB p65 and IκBα. Similar results were also obtained following the knockdown of TBK1. Furthermore, a dual luciferase reporter assay further validated that miR-19a inhibited TBK1 expression by binding directly to the 3′-UTR of TBK1.

Conclusion

We demonstrated that miR-19a has anti-inflammatory effects and mediates the negative regulation of the NF-κB Pathway in LPS-induced endometritis by targeting TBK1.

     

Annexin A3 and cancer

Annexin A3 (ANXA3), an annexin family member, contains 36 kDa and 33 kDa isoforms. Similar to other annexin members, ANXA3 plays an important role in the development of human diseases. Recent studies have reported that abnormal ANXA3 expression is closely associated with the development, progression, metastasis, drug resistance and prognosis of several malignant tumours, such as breast cancer, lung cancer and hepatocellular carcinoma. ANXA3 exerts its role by regulating cell proliferation, migration and apoptosis via the phosphatidylinositol-3 kinase/Akt, nuclear factor-κB (NF-κB), c-JUN N-terminal kinase, extracellular signal-regulated kinase and hypoxia-inducible factor-1 signalling pathways. ANXA3 may act as a novel target for the early diagnosis and treatment of tumours. The present review summarises the recent progress in the role of ANXA3 and its regulatory pathways in tumours.     

简化版动物命名试验对肝硬化并发轻微性肝性脑病的诊断价值及相关因素分析

目的 探讨应用简化版动物命名试验(S-ANT)诊断肝硬化并发轻微性肝性脑病(MHE)的价值。方法 2020年7月～2021年6月于蚌埠医学院第一附属医院感染病科和阜阳市第二人民医院肝病科住院治疗的142例肝硬化患者,采用数字连接试验A(NCT-A)、数字符号试验(DST)、动物命名试验(ANT)筛查肝硬化并发MHE患者。常规检测血常规、血生化常规和血氨,应用多因素Logistic回归分析肝硬化并发MHE的相关因素。绘制受试者工作特征(ROC)曲线,计算曲线下面积(AUC),评价S-ANT诊断MHE的效能。结果 在142例肝硬化患者中,发生MHE者39例(27.5%);MHE组患者年龄、受教育年限和酒精性肝病占比与无MHE组比,差异显著(P<0.05);MHE组血氨水平为61.0(36.0～94.0)μmol/L,显著高于无MHE组【(47.1(33.0～69.0)μmol/L,P<0.05】;MHE组NCT-A用时为(93.5±38.9)s,显著长于无MHE组【(61.5±23.7)s,P<0.05】,DST、ANT和S-ANT评分分别为(22.7±5.7)分、(12...