Onyx embolisation of cavernous sinus dural arteriovenous fistula via direct percutaneous transorbital puncture

The cavernous sinus dural arteriovenous fistulas of three patients were successfully embolised by using Onyx (Onyx Liquid Embolic System, MTI, Irvine, CA, USA) as the sole embolic agent, through direct percutaneous transorbital punctures of the cavernous sinuses. Our early experience suggests that this direct approach, coupled with the unique physical properties of Onyx, is a safe and effective alternative to treat cavernous sinus dural arteriovenous fistulas when the conventional transvenous routes are inaccessible.     

人脑胶质瘤细胞株SHG-44放射敏感性的测定方法探讨

目的 探讨MTT法、细咆计数Kit-8(CCK-8)法在进行人脑胶质瘤细胞株SHG-44细胞放射敏感性测定时能否替代集落形成试验法。方法 运用3种方法分别在0、1、2、3、4、6、8、10(3y8个不同剂量点照射后检测其存活分数，采用统计学检查分析3种方法所得存活分数的相关性。结果 在一定照射剂量内(照射剂量≤3Gy时)，MTT法、CCK-8法与克隆形成法相关性较好，而超出该剂量范围时的相关性差。经直线回归分析，3种方法均不存在高度线性相关。CCK-8和MTT法相比无更好的相关性。结论 集落形成法仍然是测定放射敏感性的“金标准”，MTT法等其他方法可以提供参考，但无法替代集落形成法。     

Serotonin content of platelets in inflammatory rheumatic diseases

Significantly decreased platelet serotonin contents were measured in rheumatoid arthritis, systemic lupus erythematosus (SLE), progressive systemic sclerosis, and mixed connective tissue disease. An inverse relationship between platelet serotonin levels and clinical disease activity was observed in both rheumatoid arthritis and systemic lupus erythematosus. SLE patients with multiple organ involvement showed the lowest platelet serotonin values. No correlation was observed between platelet serotonin contents and nonsteroidal antiinflammatory drug treatment, presence of circulating platelet reactive IgG, or the amount of circulating immune complexes. The results are interpreted as indicating platelet release occurring in vivo during inflammatory episodes of the rheumatic disorders investigated.     

Relative oral bioavailability of 3-MCPD from 3-MCPD fatty acid esters in rats

In order to quantify the relative oral bioavailability of 3-chloropropane-1,2-diol (3-MCPD) from 3-MCPD fatty acid diesters in vivo, 1,2-dipalmitoyl-3-chloropropane-1,2-diol (3-MCPD diester) and 3-MCPD were orally applied to rats in equimolar doses. In both cases, the time courses of 3-MCPD concentrations were measured in blood, various organs, tissues and intestinal luminal contents. The results show that 3-MCPD is released by enzymatic hydrolysis from the 3-MCPD diester in the gastrointestinal tract and distributed to blood, organs and tissues. Based on the measurements in blood, the areas under the curve (AUC) for 3-MCPD were calculated. By comparing both AUC, the relative amount of 3-MCPD bioavailable from the 3-MCPD diester was calculated to be 86 % on average of the amount bioavailable following administration of 3-MCPD. In view of limited experimental data, it is justified for the purpose of risk assessment to assume complete hydrolysis of the diesters in the gastro-intestinal tract. Therefore, assessment of the extent of exposure to 3-MCPD released from its fatty acid esters should be performed in the same way as exposure to the same molar quantity of 3-MCPD.     

老年呼吸道感染患者肺炎支原体抗体检测的调查分析

目的了解老年呼吸道感染患者肺炎支原体（Mycoplasmapneumoniae,MP）感染情况。方法选取广州市红十字会医院2010年1月至2012年9月≥65岁的呼吸道感染患者3765例,男2136例,女1629例,年龄在65～110岁以内,采用间接免疫荧光法（indirectimmunofluorescenceassay,IFA）对其血清中的肺炎支原体抗体（MP—IgM）进行检测。结果3765例老年呼吸道感染患者血清标本中,检出阳性的标本为834例,阳性率为22．15％,其中男性阳性率为21．11％,而女性阳性率为23、51％,不同性别间比较差异无统计学意义（X2=3．080,P〉0．05）。肺炎支原体抗体（MP—IgM）各年龄组问阳性率比较差异无统计学意义（X2=0．090,P〉0．05）。老年呼吸道感染患者肺炎支原体感染阳性率存在季节差异（X2=11．040,P〈o．05）,春季、夏季和冬季的老年呼吸道感染患者MP感染率之间比较差异无统计学意义（P〉0．05）,而春季、夏季和冬季的老年呼吸道感染患者MP感染率分别与秋季比较差异有统计学意义（P〈0．05）。结论老年呼吸道感染患者有一定的MP感染率,不同性别间差异无统计学意义,感染存在季节的差异性,因老年呼吸道感染患者感染MP合并有基础性疾病时可能会引起严重的损害,故临床应重视老年呼吸道感染患者MP的感染和防治,早发现早治疗;