In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation

The complex process of spermatogenesis is regulated by various factors. In the present study, the in vitro effects of epidermal growth factor (EGF), follicle stimulating hormone (FSH) and testosterone on spermatogonial cell colony formation were investigated, and the best colonising factor was chosen for treating cells before transplantation. Sertoli and spermatogonial cells were isolated from neonatal mouse testes. The identity of the cells was confirmed through analysis of morphology, alkaline phosphatase activity, immunoreactivity and transplantation. Co-cultured Sertoli and spermatogonial cells were treated with EGF, FSH and testosterone before colony assay. Results indicated that EGF is the best factor for in vitro colonisation of spermatogonial cells, but transplantation of the EGF-treated group did not show any significant change compared with the control groups. In conclusion, EGF increased in vitro colonisation of spermatogonial cells, but, as a result of differential effects, did not influence transplantation efficiency.     

Bone microenvironment modulates expression and activity of cathepsin B in prostate cancer

Prostate cancers metastasize to bone leading to osteolysis. Here we assessed proteolysis of DQ-collagen I (a bone matrix protein) and, for comparison, DQ-collagen IV, by living human prostate carcinoma cells in vitro. Both collagens were degraded, and this degradation was reduced by inhibitors of matrix metallo, serine, and cysteine proteases. Because secretion of the cysteine protease cathepsin B is increased in human breast fibroblasts grown on collagen I gels, we analyzed cathepsin B levels and secretion in prostate cells grown on collagen I gels. Levels and secretion were increased only in DU145 cells--cells that expressed the highest baseline levels of cathepsin B. Secretion of cathepsin B was also elevated in DU145 cells grown in vitro on human bone fragments. We further investigated the effect of the bone microenvironment on cathepsin B expression and activity in vivo in a SCID-human model of prostate bone metastasis. High levels of cathepsin B protein and activity were found in DU145, PC3, and LNCaP bone tumors, although the PC3 and LNCaP cells had exhibited low cathepsin B expression in vitro. Our results suggest that tumor-stromal interactions in the context of the bone microenvironment can modulate the expression of the cysteine protease cathepsin B.     

Promoting environmental justice through community-based participatory research: the role of community and partnership capacity.

Community-based participatory research (CBPR) increasingly is being used to study and address environmental justice. This article presents the results of a cross-site case study of four CBPR partnerships in the United States that researched environmental health problems and worked to educate legislators and promote relevant public policy. The authors focus on community and partnership capacity within and across sites, using as a theoretical framework Goodman and his colleagues' dimensions of community capacity, as these were tailored to environmental health by Freudenberg, and as further modified to include partnership capacity within a systems perspective. The four CBPR partnerships examined were situated in NewYork, California, Oklahoma, and North Carolina and were part of a larger national study. Case study contexts and characteristics, policy-related outcomes, and findings related to community and partnership capacity are presented, with implications drawn for other CBPR partnerships with a policy focus.     

The Iranian version of Angina Pectoris characteristics questionnaire: reliability assessment

Aims. To evaluate internal consistency and test–retest reliability of the Iranian version of the Angina Pectoris characteristics questionnaire. Background. To evaluate symptoms in patients with Angina Pectoris, the Iranian version of the Angina Pectoris characteristics questionnaire was created by combining items from Modified Rose Questionnaire, Short Form‐McGill Questionnaire and the Accompanying Symptom Checklist. The Angina Pectoris characteristics questionnaire has been developed and validated recently although it required assessment of its reliability. Design. Reliability study using test and retest. Method. One hundred and ten coronary heart disease patients with Angina Pectoris were included. The test–retest reliability of the instrument was estimated for measurement after 15 days. Result. The analysis of the result indicated that internal consistency was found adequate at both assessments (Cronbach’s alpha = 0·65) for test and retest. There was no significant difference between test and retest. The intra‐class correlation coefficient ranged from 0·76–0·98. Conclusion. The Angina Pectoris characteristics questionnaire is a reliable instrument for the assessment of Angina Pectoris characteristics in a Persian population. Relevance to clinical practice. The Angina Pectoris characteristics questionnaire can be used with confidence in Iranian coronary heart disease patients for Assessing Angina Pectoris symptom.