睾丸内白膜下注射法,一种雄性节育药的初筛模型

探索了一种简易男性节育药的筛选方法,将少量样品注射到大鼠睾丸白膜下,共2次,于第3周末解剖大鼠,作附睾精子、睾丸和附睾组织学检查。该法比WHO制订的MB-50分析法简单、省时、用药量少。药物注入后可在睾丸内进行部分代谢,因此,具有抗生育活性的代谢产物也能得到阳性结果。共筛选了近200个样品,并与MB-50比较,结果基本一致,仅出现个别假阳性并无假阴性,因此可以作为初筛方法。     

Evaluation of a virosomal H5N1 vaccine formulated with Matrix M™ adjuvant in a phase I clinical trial

The avian influenza H5 virus epizootic continues to cause zoonosis with human fatalities, highlighting the continued need for pandemic preparedness against this subtype. This study evaluated the tolerability and immunogenicity of a Matrix M™ adjuvanted virosomal H5N1 vaccine in a phase I clinical trial. Sixty healthy adults were vaccinated intramuscularly with two doses of influenza H5N1 (NIBRG-14) virosomal vaccine alone (30 μg haemagglutinin (HA)) or 1.5, 7.5 or 30 μg HA formulated with 50 μg Matrix M™ adjuvant. The antibody response was analysed by haemagglutination inhibition (HI), microneutralisation (MN) and single radial haemolysis (SRH) assays. The vaccine was well tolerated in all groups but injection site pain was more frequently observed in the Matrix M™ adjuvanted groups. The vaccine elicited homologous and heterologous H5N1-specific antibody responses and the Matrix M™ adjuvanted formulations met all the EU regulatory criteria. In conclusion, Matrix M™ adjuvant was well tolerated and augmented the antibody response allowing considerable dose sparing down to 1.5 μg HA.     

Five Successful Deliveries Following 9 Consecutive Spontaneous Abortions in a Patient with Wilson Disease

EDITORIAL COMMENT: We accepted this case for publication because it surely presents the ultimate evidence of an association between recurrent spontaneous abortion and untreated Wilson disease, in a woman with great reproductive stamina.     

CCU病房患者应激状态下发生精神异常2例

1　临床资料　患者,A,男,81岁,西安市人,农民,主因间断胸闷、气短、双下肢浮肿8mo余,加重10d于1999-12-10急诊收住西京医院心内科CCU病房.入院查体:BP19.3/13.3kPa,神志清,端坐位呼吸,查体合作,口唇紫绀,肝颈反流征(+),双肺布满干湿罗音,以中下肺野中小水泡音为主,心界左扩,心率75次·min-1,律齐,心前区可闻及级收缩期杂音,肝大肋下2指,生理反射存在,病理反射未引出.入院后心电图示:心肌供血不足.胸片提示:心影大,心胸比0.69,肺瘀血.入院诊断:1高血压病级、高心病、急性左心衰.2冠心病.1999-12-12因目睹邻床青年患者病危抢救全过程,于当…     

肠道产丁酸细菌及其丁酸产生机制的研究进展

哺乳动物大肠中的厌氧微生物能发酵产生大量的短链脂肪酸(SCFA),包括乙酸、丙酸和丁酸,其中丁酸是结肠能量的首选来源,对肠黏膜修复及结肠炎和结肠癌的预防起作用．近年来,随着分子生物学技术的发展,关于肠道产丁酸菌种类、数量及发酵特性的研究有了很大进展．本文就单胃动物肠道产丁酸菌种类、丁酸产生途径的研究新进展进行综述．     

The humoral and cellular responses induced locally and systemically after parenteral influenza vaccination in man

Studies of the immune response after influenza vaccination in man, with focus on the immune activity occurring locally at mucosal surfaces and in associated lymphoid tissue, provide a valuable insight into immunity to influenza. The aim of influenza vaccination is to develop immunological memory resulting in enhanced rapid specific response upon subsequent influenza encounter. The tonsils are thought to play an important role as an activating, effector and memory site for immune responses against influenza. We have shown that normally high numbers of influenza-specific antibody secreting cells (ASC) are present in the nasal mucosa of healthy adults but upon parenteral vaccination the numbers remain stable. However, a rapid transient increase in influenza-specific ASC is observed in the tonsils and peripheral blood after vaccination. In the tonsils and blood, parenteral vaccination results in a significant decrease in CD4(+) cells upon vaccination, which are probably recruited to the draining lymph node.