Agilent 2100 Bioanalyzer在人乳头瘤病毒检测中的应用

目的：探讨Agilent2100Bioanalyzer芯片分析系统（简称Bioanalyzer)在人乳头瘤病毒（HPV）的PCR检测和分组中的应用。方法：先分别进行通用引物介导PCR（GP－PCR）和型特异性引物介导PCR，扩增HPV高保守区（L1区）的共同序列和各型（包括HPV6，11，16和18型）的特型性序列，然后分别用常规的琼脂糖凝胶电泳技术和Bioanalyzer对PCR产物进行检测，并比较两种检测方法的准确度，重复性和灵敏度。结果：Bioanalyzer在确定片段长度时的准确度和重复性比琼脂糖凝胶电泳高，前者的准确度在95％以上，后者仅为85％，Bioanalyzer的检测灵敏度比琼脂糖凝胶电泳高100倍，结论：Bioanalyzer芯片分析系统结合PCR方法可对HPV进行特异，准确，稳定，灵敏的检测和型别鉴定，具有重要的推广价值。     

运用心理护理程序开展无偿献血者心理护理的现状

按心理护理程序实施步骤综述了采供血机构开展无偿献血心理护理的现状,提出应尽快拓宽采供血机构医护人员心理学知识,提高心理护理技能水平,构建科学合理的采供血机构心理护理模式.     

国产奎硫平合并舒必利治疗难治性精神分裂症

目的:了解舒必利合并国产奎硫平(启维)治疗难治性精神分裂症的疗效和安全性。方法:26例符合CCMD-Ⅲ精神分裂症诊断标准且临床判断属于难治性病人,予舒必利合并奎硫平治疗,疗程8周,分别在治疗前及治疗后进行阳性与阴性症状量表(PANSS)和不良反应状量表(TESS)评定。结果:合并治疗8周后总有效率65.3%。PANSS阳性、阴性及一般精神病理评分于治疗前后均有显著差异(P<0.01)。TESS总分治疗前后无显著差异(P>0.05)。副反应主要为肌张力增高、失眠、体重增加、静坐不能。结论:对于舒必利治疗反应不佳的难治性精神分裂症病人合并奎硫平治疗仍可取得较好的临床效果,安全性较高,耐受性及依从性好,可作为临床治疗难治性病人的方法之一。     

Antiproliferative Activity Against MCF‐7 Breast Cancer Cells by Diamino‐Triazaspirodiene Antifolates

Two triazaspirodienes, having similar phenoxy propyloxy side chain, were identified as potent mammalian dihydrofolate reductase inhibitors; one having a 6,5‐spiro bicyclic ring system (IC₅₀ = 2.3 nm ) and the other a 6,6‐spiro bicyclic system (IC₅₀ = 6.9 nm ). They also showed more than 50% antiproliferative activity against the MCF‐7 breast cancer cells at 20 μm . This study demonstrated the potential lead of the diamino‐triazaspirodienes in anticancer chemotherapeutical agents’ discovery.     

我院护士工作评估体系及其应用

目的应用护士工作评估体系,激励护士不断自我完善.方法以自我评估、面对面交流、同事间评估、设立目标和建立护士档案为主要内容的评估.一般为每年1次.结果每年护士评估的覆盖率为100%.提供了上下级面对面交流的机会,每名护士都熟悉评估的程序并自觉参与.结论护士评估体系是护理管理的重要组成部分,它使管理者有机会关注到每名护士的成长需要及成长目标,指导我们能动地利用人力--这一最宝贵的资源.     

尿石症住院患者1100例分析

目的了解近年来尿石症住院患者情况的变化。方法对1998年~2003年间的尿石症住院患者1100例进行分析。结果本组中,肾结石251例(22.8%)、输尿管结石742例(67.5%)、膀胱结石97例(8.8%)、尿道结石10例(0.9%)。上、下尿路结石的比例为9.28∶1。高钙尿患者27例(13.0%)、高钙血症91例(9.1%)、高尿酸血症167例(17.8%)。结石成分分析结果:草酸钙168例(62.7%);磷酸钙59例(22.0%);尿酸及尿酸盐25例(9.3%);磷酸镁铵10例(3.7%);碳酸盐5例(1.7%);胱氨酸1例(0.4%)。治疗:ESWL658例(59.8%)、肾切开取石术35例、肾切除术6例、输尿管切开取石术92例(占8.4%)、输尿管镜加气压弹道碎石52例(4.7%)、膀胱切开取石53例(其中前列腺摘除术加膀胱切开取石术26例);膀胱镜加气压弹道碎石20例;前列腺电切术加气压弹道碎石7例;膀胱镜加大力钳碎石术9例。尿道切开取石术1例;经尿道镜取石术2例;尿道镜加气压弹道碎石3例。结论本组尿石症患者以上尿路结石为主,含钙结石占绝大多数。尽管微创手术的普遍开展,ESWL仍不失为一种创伤小、效果好的治疗方法。     

Association of the GNAS1 gene variant with hypertension is dependent on alcohol consumption.

The beta-adrenoceptor (beta-AR)-stimulatory guanine nucleotide-binding (Gs) protein system has been shown to play important roles in the cardiovascular system. The gene encoding the alpha-subunit of Gs proteins (GNAS1) is a candidate genetic determinant for hypertension. Because alcohol consumption is known to affect blood pressure partly through the beta-AR-Gs protein system, we examined the possible interaction between GNAS1 T393C polymorphism and drinking status in the association with hypertension in the present study. As a result, a non-significant but reasonable trend supporting the presence of an interaction was shown (p = 0.076). In line with this trend, the T393C polymorphism significantly interacted with drinking status in the association with systolic blood pressure (p = 0.028). Moreover, supporting the presence of an interaction, T allele carriers consistently had a higher probability of hypertension, higher systolic blood pressure, and higher diastolic blood pressure than CC homozygotes in non-drinkers and light drinkers. In contrast, CC homozygotes consistently had a higher probability of hypertension, higher systolic blood pressure, and higher diastolic blood pressure than T allele carriers in moderate to heavy drinkers. The present study also showed a significant interaction between the T393C polymorphism and drinking status in the association with pulse pressure (p = 0.026), reflected by a significant association between the T393C polymorphism and pulse pressure in moderate to heavy drinkers (p = 0.026). These findings may be helpful in conducting further molecular and biological studies on the relationship among the effects of alcohol, the beta-AR-Gs protein system, and hypertension.     

A cyclic peptide–polymer probe for the detection of Clostridium botulinum neurotoxin serotype A

A Botulinum neurotoxin serotype A (BoNT/A) ELISA detection system was developed based upon an 11-mer cyclic peptide, termed C11-019, that was identified through peptide phage display technology. The assay employs a sandwich format using the C11-019 cyclic peptide attached to a PEMA (poly(ethylene maleic anhydride)) matrix as the capture phase and anti-BoNT/A polyclonal antibodies as the detection phase. Results reported demonstrate that the C11-019 peptide–polymer can specifically bind to BoNT/A with no cross-reactivity to other serotypes examined in assay buffers and a variety of body fluids and foodstuffs. When a highly sensitive chemiluminescent substrate was engaged, the detection of 1 pg/mL could be readily achieved within 3 h with a linear range of 0.1–1 ng/mL. These results demonstrate that an inexpensive peptide–polymer-based capture ELISA system can be used for rapid, sensitive and highly specific BoNT detection.     

类风湿病中左心室收缩功能障碍：一种未被认识的负荷？

目的:本研究旨在明确左心室收缩功能障碍在临床类风湿病(RD)患者中是否较正常人群中更常见,并评价脑利钠肽(BNP)的诊断价值。背景:RD患者罹患缺血性心脏病的风险增加,而采用超声心动图评估RD患者心功能不全的大型研究却少见。假定左心室收缩功能障碍在RD患者中较正常人群中更为