全文获取类型
收费全文 | 505篇 |
免费 | 57篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 10篇 |
儿科学 | 48篇 |
妇产科学 | 6篇 |
基础医学 | 56篇 |
口腔科学 | 27篇 |
临床医学 | 57篇 |
内科学 | 115篇 |
皮肤病学 | 19篇 |
神经病学 | 19篇 |
特种医学 | 92篇 |
外科学 | 33篇 |
综合类 | 17篇 |
预防医学 | 9篇 |
药学 | 34篇 |
中国医学 | 1篇 |
肿瘤学 | 24篇 |
出版年
2023年 | 1篇 |
2022年 | 6篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 13篇 |
2014年 | 17篇 |
2013年 | 22篇 |
2012年 | 13篇 |
2011年 | 16篇 |
2010年 | 40篇 |
2009年 | 27篇 |
2008年 | 15篇 |
2007年 | 11篇 |
2006年 | 11篇 |
2005年 | 15篇 |
2004年 | 11篇 |
2003年 | 12篇 |
2002年 | 11篇 |
2001年 | 12篇 |
2000年 | 5篇 |
1999年 | 12篇 |
1998年 | 28篇 |
1997年 | 34篇 |
1996年 | 34篇 |
1995年 | 27篇 |
1994年 | 18篇 |
1993年 | 17篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 12篇 |
1988年 | 13篇 |
1987年 | 17篇 |
1986年 | 10篇 |
1985年 | 8篇 |
1984年 | 4篇 |
1983年 | 3篇 |
1982年 | 6篇 |
1981年 | 10篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1954年 | 1篇 |
排序方式: 共有567条查询结果,搜索用时 8 毫秒
51.
52.
Mean platelet survival and turnover were simultaneously determined with autologous 111In-labeled platelets (111In-AP) and homologous 51Cr- labeled platelets (51Cr-HP) in ten patients with chronic immune thrombocytopenic purpura (ITP). In vivo redistribution of the 111In-AP was quantitated with a scintillation camera and computer-assisted image analysis. The patients were divided into two groups: those with splenic platelet sequestration (spleen-liver 111In activity ratio greater than 1.4), and those with diffuse sequestration in the reticuloendothelial system. The latter patients had more severe ITP reflected by pronounced thrombocytopenia, decreased platelet turnover, and prominent early hepatic platelet sequestration. Mean platelet life span estimated with 51Cr-HP was consistently shorter than that of 111In-AP. Platelet turnover determined with 51Cr-HP was thus over-estimated. The difference in results with the two isotope labels was apparently due to greater in vivo elution of 51Cr. Although the limitations of the techniques should be taken into account, these findings indicate that platelet turnover is not always normal or increased in ITP, but is low in severe disease. We suggest that this may be ascribed to damage to megakaryocytes by antiplatelet antibody. The physical characteristics in 111In clearly make this radionuclide superior to 51Cr for the study of platelet kinetics in ITP. 相似文献
53.
54.
MG Pacheco-Tovar E Avalos-Díaz E Vega-Memije JJ Bollain-y-Goytia E López-Robles MT Hojyo-Tomoka L Domínguez-Soto R Herrera-Esparza 《Journal of the European Academy of Dermatology and Venereology》2009,23(6):697-701
Background Pemphigus is an autoimmune disease characterized by the formation of intra-epidermal blisters. Patients develop auto-antibodies against desmoglein 1 and 3 proteins and induce acantholysis.
Objective This work addresses the issue of whether the Fas pathway mediates acantholysis. Furthermore, the possible suppliers of the Fas pathway were investigated.
Methods Seventeen biopsies of pemphigus patients were studied by haematoxylin and eosin staining, and apoptosis was defined by TUNEL. The expression of Fas, FasL and caspase 3 was studied by in situ hybridization and immunohistochemistry. Cell infiltrates were studied by immunofluorescence with monoclonal anti-CD3, CD4, CD8, CD19 and CD69.
Results All of the biopsies showed intra-epidermal blisters, acantholytic cells and inflammatory infiltrates. The blisters expressed Fas, FasL and caspase 3. Cell infiltrates were composed of CD8 and a few CD4+ CD69+ cells. Additionally, CD19+ cells were detected. Interestingly, the Fas expression was increased in acantholytic cells and perilesional keratinocytes. Incidentally, these cells exhibited apoptotic features. Interestingly, the CD8 cells expressed FasL.
Conclusion This paper presents the morphological evidence that apoptosis and acantholysis are linked. Therefore, the Fas pathway is associated with CD8 cells in pemphigus lesions.
None declared. 相似文献
Objective This work addresses the issue of whether the Fas pathway mediates acantholysis. Furthermore, the possible suppliers of the Fas pathway were investigated.
Methods Seventeen biopsies of pemphigus patients were studied by haematoxylin and eosin staining, and apoptosis was defined by TUNEL. The expression of Fas, FasL and caspase 3 was studied by in situ hybridization and immunohistochemistry. Cell infiltrates were studied by immunofluorescence with monoclonal anti-CD3, CD4, CD8, CD19 and CD69.
Results All of the biopsies showed intra-epidermal blisters, acantholytic cells and inflammatory infiltrates. The blisters expressed Fas, FasL and caspase 3. Cell infiltrates were composed of CD8 and a few CD4
Conclusion This paper presents the morphological evidence that apoptosis and acantholysis are linked. Therefore, the Fas pathway is associated with CD8 cells in pemphigus lesions.
Conflicts of interest
None declared. 相似文献
55.
56.
Nuclear RNA sequences coding for alpha and beta globins in erythroid cells: evidence for multiple intermediate molecules 总被引:1,自引:0,他引:1
The poly (A)-containing nuclear RNA from dimethylsulfoxide-induced Friend leukemia cells was fractionated by acrylamide gel electrophoresis in denaturing conditions and analyzed for alpha and beta globin RNA sequences. The results indicate that nuclear RNA contains one species of large-size RNA (0.6 X 10(6) daltons), which is the putative precursor for beta globin mRNA only. In addition, it was shown by electrophoretic analysis that the complex of RNA molecules not resolved by sucrose gradient centrifugation (11S) comprises sequences of decreasing size (0.34, 0.28, and 0.26 X 10(6) daltons), which might be the precursors of alpha and beta globin mRNA. 相似文献
57.
Pre‐therapy inflammation and coagulation activation and long‐term CD4 count responses to the initiation of antiretroviral therapy 下载免费PDF全文
58.
59.
Cytomegalovirus vector expressing RAE‐1γ induces enhanced anti‐tumor capacity of murine CD8+ T cells 下载免费PDF全文
Tihana Tršan Kristina Vuković Petra Filipović Ana Lesac Brizić Niels A. W. Lemmermann Kilian Schober Dirk H. Busch William J. Britt Martin Messerle Stipan Jonjić 《European journal of immunology》2017,47(8):1354-1367
Designing CD8+ T‐cell vaccines, which would provide protection against tumors is still considered a great challenge in immunotherapy. Here we show the robust potential of cytomegalovirus (CMV) vector expressing the NKG2D ligand RAE‐1γ as CD8+ T cell‐based vaccine against malignant tumors. Immunization with the CMV vector expressing RAE‐1γ, delayed tumor growth or even provided complete protection against tumor challenge in both prophylactic and therapeutic settings. Moreover, a potent tumor control in mice vaccinated with this vector can be further enhanced by blocking the immune checkpoints TIGIT and PD‐1. CMV vector expressing RAE‐1γ potentiated expansion of KLRG1+ CD8+ T cells with enhanced effector properties. This vaccination was even more efficient in neonatal mice, resulting in the expansion and long‐term maintenance of epitope‐specific CD8+ T cells conferring robust resistance against tumor challenge. Our data show that immunomodulation of CD8+ T‐cell responses promoted by herpesvirus expressing a ligand for NKG2D receptor can provide a powerful platform for the prevention and treatment of CD8+ T‐cell sensitive tumors. 相似文献
60.