Identification of abnormally methylated–differentially expressed genes and pathways in osteoarthritis: a comprehensive bioinformatic study

Clinical Rheumatology - To investigate abnormally methylated–differentially expressed genes (DEGs) and their related pathways in osteoarthritis (OA) by comprehensive bioinformatic analysis....     

Controlled Ovarian Stimulation Using Medroxyprogesterone Acetate and hMG in Patients With Polycystic Ovary Syndrome Treated for IVF: A Double-Blind Randomized Crossover Clinical Trial

Ovarian hyperstimulation syndrome (OHSS) during ovarian stimulation is a current challenge for patients with polycystic ovarian syndrome (PCOS). Our previous studies indicated that progestin can prevent premature luteinizing hormone (LH) surge or moderate/severe OHSS in the general subfertile population, both in the follicular-phase and luteal-phase ovarian stimulation but it is unclear if this is true for patients with PCOS.The aim of the article was to analyze cycle characteristics and endocrinological profiles using human menopausal gonadotropin (hMG) in combination with medroxyprogesterone acetate (MPA) for PCOS patients who are undergoing IVF/intracytoplasmic sperm injection (ICSI) treatments and investigate the subsequently pregnancy outcomes of frozen embryo transfer (FET).In the randomized prospective controlled study, 120 PCOS patients undergoing IVF/ICSI were recruited and randomly classified into 2 groups according to the ovarian stimulation protocols: hMG and MPA (group A, n = 60) or short protocol (group B, n = 60).In the study group, hMG (150–225IU) and MPA (10 mg/d) were administered simultaneously beginning on cycle day 3. Ovulation was cotriggered by a gonadotropinreleasing hormone (GnRH) agonist (0.1 mg) and hCG (1000IU) when dominant follicles matured. A short protocol was used as a control.The primary end-point was the ongoing pregnancy rate per transfer and incidence of OHSS.Doses of hMG administrated in group A are significantly higher than those in the controls. LH suppression persisted during ovarian stimulation and no incidence of premature LH surge was seen in both groups. The fertilization rate and the ongoing pregnant rate in the study group were higher than that in the control. The number of oocytes retrieved, mature oocytes, clinical pregnancy rates per transfer, implantation rates, and cumulative pregnancy rates per patient were comparable between the 2 groups. The incidence of OHSS was low between the 2 groups, with no significant difference.The study showed that MPA has the advantages of an oral administration route, easy access, more control over LH levels. A possible reduction in the incidence of moderate or severe OHSS with the MPA protocol should be viewed with caution as the data is small. Large randomized trials with adequate sample size remain necessary.     

柚皮挥发油的β-环糊精包合工艺研究

目的确定柚皮中挥发油β-环糊精包合工艺条件。方法 以挥发油包合率、包合物收率为指标，采用正交试验方法优选制备挥发油B一环糊精的最佳包合工艺条件。结果 最佳包合工艺条件为挥发油与β-环糊精比例1：6（mL：g），提取温度50℃，搅拌时间2h。结论 用优选的包合工艺条件制备的包合物包合率及收率较高。     

益心解毒颗粒对动物心律失常的保护作用

目的：观察益心解毒颗粒对由CaCl2和乌头碱诱发动物心律失常的保护作用。方法：于大鼠末次用药60min后，舌下注射CaCl2和乌头碱诱发大鼠心律失常，观察大鼠心电图变化及心律失常情况。结果：益心解毒颗粒3g／kg可明显提高CaCl2致大鼠心律失常的存活率；益心解毒颗粒1．5～6．0g／kg可推迟乌头碱致大鼠心律失常的发生时间、缩短心律失常持续时间。结论：益心解毒颗粒对动物心律失常有明显的保护作用。     

Spinal epidural abscess successfully treated with percutaneous,computed tomography-guided,needle aspiration and parenteral antibiotic therapy: case report and review of the literature

OBJECTIVE AND IMPORTANCE: We describe a case of a posterior spinal epidural abscess that was successfully treated with percutaneous, computed tomography-guided, needle aspiration and parenteral antibiotic therapy. CLINICAL PRESENTATION: A 48-year-old man experienced acute pain in the paralumbar region for 1 week, followed by gait disturbance and micturition difficulty. Laboratory studies demonstrated leukocytosis and hyperglycemia. Blood cultures yielded Staphylococcus aureus. Magnetic resonance imaging scans of the thoracolumbar spine revealed a posterior spinal epidural abscess located between L2 and the lower cervical spine. INTERVENTION: The patient's low back pain persisted despite 10 days of antibiotic therapy. Therefore, percutaneous, computed tomography-guided, needle aspiration was performed. The low back pain was relieved dramatically and immediately after the procedure. The spinal epidural abscess completely resolved after 6 weeks of antibiotic treatment. CONCLUSION: Surgical decompression and antibiotic therapy are the treatments of choice for patients with spinal epidural abscesses. Selected patients may be treated nonsurgically. Rarely, percutaneous drainage of the abscess has been reported to be helpful. Our case suggests that percutaneous, computed tomography-guided, needle aspiration might be a rational alternative to surgical decompression for treatment of spinal epidural abscesses.     

SunghyangJungki-San Ga Pogongyoung inhibits IL-1 mediated tumour necrosis factor-alpha secretion in astrocytes

Astrocytes play an important role in initiating and modulating inflammatory responses within the central nervous system (CNS). Extensive studies in rodents have shown that substance P induces inflammatory cytokine production in astrocytes. In this study we have examined whether an aqueous extract of SunghyangJungki-San Ga Pogongyoung (SSGP) inhibits the secretion of TNF-alpha from primary cultures of rat astrocytes. SSGP (10-1,000 microg/mL) significantly inhibited the TNF-alpha secretion by astrocytes stimulated with lipopolysaccharide (LPS) and substance P (SP). Interleukin-1 (IL-1) has been shown to elevate TNF-alpha secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore examined whether IL-1 mediated inhibition of TNF-alpha secretion from primary astrocytes by SSGP. Treatment with SSGP (10-1,000 microg/mL) to astrocytes stimulated with both LPS and SP decreased IL-1 secretion significantly. Moreover, the secretion of TNF-alpha by LPS and SP in astrocytes was progressively inhibited with an increasing amount of IL-1 neutralizing antibody. Our results suggest that SSGP may inhibit TNF-alpha secretion by inhibiting IL-1 secretion and that SSGP has an antiinflammatory activity in the CNS.     

A longitudinal cardiovascular autonomic function study in mild Guillain-Barré syndrome

To identify subclinical autonomic dysfunction in mild Guillain-Barré syndrome (GBS), a set of autonomic function tests was serially performed for up to 6 months in 5 GBS patients with mild disability at the nadir. Parasympathetic autonomic function tests consisted of Valsalva ratio and R-R interval variation during rest and deep breathing. Sympathetic autonomic function was evaluated by blood pressure responses to sustained handgrip, hand immersion in ice water, and active standing. The results showed that abnormal parasympathetic and sympathetic function was frequently encountered in all 5 patients during the acute stage of the illness. Autonomic dysfunction occurred both in axonal and demyelinating types of GBS. There was a trend of improvement in most autonomic function tests after 3 months, comparable to the recovery of motor function. In conclusion, subclinical autonomic dysfunction was present in mild GBS. It was temporary and would resolve spontaneously.     

《中国药典》2005年版二部庆大霉素[鉴别]项中薄层分析浓度的探讨

一、仪器与试药 仪器：METLER AE 240 电子分析天平（十万分之一）；硅胶G由青岛海洋化工厂提供。 药品：庆大霉素标准品（由中国药品生物制品检定所提供）硫酸庆大霉素注射液分别为（山东瑞阳制药有限公司02101401；天津药业集团新郑股份有限公司030220）     

警察院校学生德育课教学的心理问题及其调适

从多年的德育课教学实践看,警察院校学生对德育课的逆反心理和学生的超前心理障碍,对他们的正常学习、健康及思想觉悟的提高影响极大。研究这2种心理障碍,完善教学方法,调动学员学习主观能动性是摆在警察院校德育教师面前的一个重要课题。1学员对德育课逆反心理的产生、表现所谓逆反心理是客观环境与主体需要不相符合时产生的具有强烈抵触情绪的社会态度,具有多样的表现形式。通过对我院学生的问卷调查,对德育课表现出来的逆反心理主要可以概括为以下几种类型。1.1硬性说教逆反型由于教师对警察院校的管理原则缺乏全面理解,认为军事化管理只…     

HPLC测定复方泰痹胶囊Ⅰ号中大黄素含量

目的:HPLC测定复方泰痹胶囊Ⅰ号中大黄素含量。方法:以A lltim aC18柱,甲醇-水-磷酸(80∶20∶0.04)为流动相:流速:1.0mL/m in,检测波长:347nm。结果:大黄素线性范围:5~50μg/mL,r=0.9993,平均回收率为98.3%,RSD=2.67%(n=5)。结论:方法简便、准确、重现性好,可作为该制剂的含量测定方法。