地佐辛复合布托啡诺在剖宫产产妇术后镇痛、镇静效果及并发症的对比分析#br#

Objective?To analyze the application effects of dezocine combined with butorphanol on postoperative analgesia, sedation and complications of puerperae with cesarean section. Methods?98 patients after cesarean section treated in the First Affiliated Hospital of Henan University of CM between October 2019 and March 2021 were selected, and randomly divided into control group (n=48, treated with butorphanol) and observation group (n=50, treated with dezocine combined with butorphanol). Visual Analogue Scale (VAS), Bruggrmann Comfort Scale (BCS) and Ramsay Sedation Score (RSS) were used to compare the analgesic effects at 1 h, 6 h, 12 h and 24 h after surgery (T1-T4). Levels of C-reactive protein (CRP), interleukin-6 (IL-6) and interleukin-2 (IL-2) of the two groups were compared at T1 and T4. Incidence rate of postoperative complications was compared between the two groups. Results?VAS scores of patients in observation group at T1-T4 were lower while BCS scores were higher compared those in control group, and Ramsay sedation scores at T1-T3 were higher than those in control group, with time-point effect, between-group effect and interaction effect (P<0.05). Levels of CRP and IL-6 of observation group at T1 and T4 were lower while level of IL-2 was higher compared to control group (P<0.05). The incidence rates of complications of the two groups were 8.00% and 16.67% respectively (P>0.05). Conclusion?Dezocine combined with butorphanol has better sedative and analgesic effects on puerperae after cesarean section, and it helps to improve the comfort and relieve the body’s inflammatory response, with few complications.     

Detection of periodontal pathogens in the patients with aortic aneurysm

Background The occurrence and development of aortic aneurysm （AA） are associated with infection.Some researchers have detected the DNA of periodontal pathogens in AA samples in certain populations.However,it has not been done in Chinese population.The objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of AA patients.Methods Eighty-nine subjects with AA and 59 subjects without AA were examined.Periodontal clinical parameters were evaluated.Unstimulated saliva and subgingival plaque somples were collected from all subjects.Twenty-six dissected AA samples were obtained.Evidence of eight periodontal pathogens including Porphyromonas gingivalis （Pg）,Actinobacillus actinomycetemcomitans （Aa）,Prevotella intermedia （Pi）,Tannerella forsythensis （Tf）,Treponema denticola （Td）,Campylobacter rectus （Cr）,Fusobacterium nucleatum （Fn）,and Prevotella nigrescens （Pn） was ascertained in all samples by 16S rRNA-based polymerase chain reaction （PCR） assay.Results The periodontal indexes including plaque index （PLI）,probing depth （PD）,bleeding index （BI）,and clinical attachment loss （CAL）,of the six Ramfjord index teeth were significantly higher in the AA group than those in the control group （P ＜0.01）.Eight periodontal pathogens in subgingival plaque samples were more frequently detected in the AA group than in control group.The difference in prevalence between the groups was significant for six （out of eight） periodontal pathogens assayed （Pg,Pi,Fn,Pn,Tf,and Td,P ＜0.01）.Additionally,all eight periodontal pathogens were more frequently detected in saliva samples of the AA group than in those of the control group,again with six （out of eight） （Pg,Pi,Fn,Cr,Tf,and Td） displaying significant differences in prevalence between the two groups （P ＜0.01）.Out of 26 aneurysm samples examined,Pg,Pi,Fn,Crand Tfwere detected in 6 （23.1％）,2 （7.7％）,3 （11.5％）,1 （3.8％）,2     

彩色多普勒超声对下肢动脉支架内再狭窄的诊断价值

目的探讨彩色多普勒超声对下肢动脉支架内再狭窄（ISR）的诊断价值,为临床应用提供依据。方法选取下肢动脉支架植入术后12个月内,经CT血管造影（CTA）或数字减影血管造影（DSA）证实的再狭窄患者31例（植入支架再狭窄47处）、未狭窄患者63例（植入支架89处）及健康对照者30名行超声检查,获取支架内、支架近心端及远心端自体血管的二维超声声像图特点及收缩期峰值血流速度（PSV）、收缩期血流加速时间（AT）,与健康对照者相应血管血流参数作比较。应用受试者操作特性（ROC）曲线分析PSV、AT对下肢动脉ISR的诊断价值。结果未狭窄组股总动脉、股浅动脉、腘动脉支架内PSV分别为（146.71±35.59）、（120.11±25.67）、（96.44±32.87）cm/s,健康对照组上述指标分别为（119.67±15.34）、（91.17±15.09）、（71.13±21.23）cm/s,组间比较,差异均有统计学意义（t=2.457、2.459、2.321,P均〈0.05）;未狭窄组股总动脉、股浅动脉、腘动脉AT分别为（84.98±13.77）、（87.33±16.36）、（90.77±12.05）ms;健康对照组上述指标分别为（78.23±21.24）、（82.31±18.24）、（84.29±23.01）ms,组间比较,差异均无统计学意义（t=1.696、1.904、1.835,P均〉0.05）。再狭窄组支架近心端、狭窄处、远心端PSV分别为（87.67±23.34）、（218.17±72.09）、（54.13±21.23）cm/s,未狭窄组支架近心端、支架内、远心端PSV分别为（91.71±25.59）、（131.11±45.67）、（96.44±32.87）cm/s,组间比较,支架内/狭窄处及支架远心端PSV的差异有统计学意义（t=3.412、3.511,P均〈0.01）,支架近心端PSV差异无统计学意义（t=1.901,P〉0.05）;再狭窄组支架近心端、狭窄处、远心端AT分别为（98.31±14.09）、（109.54±21.03）、（158.23±45.21）ms,未狭窄组支架近心端、支架内、远心端AT分别为（84.98±13.77）、（86.34±19.36）、（83.77±17.05）ms,组间比较,支架内/狭?     

Study on screening and evaluation methods of cosmetics for people with facial sensitive skin

Objective:The purpose of this study is to screen and evaluate cosmetic products for sensitive skin on the face.Methods:Thirty-five subjects with positive lactic acid sting test (LAST) were recruited from the staff of our hospital from November 2019 to February 2020. First, the human skin enclosed patch test of cosmetic gel (abbreviated as gel) was performed, and then the tested products were continuously applied for 4 weeks to complete the long-term efficacy test. Subjects’ sensation of application, pruritus, tingling and burning were assessed on a 0 to 9 scale prior to, 14, and 28 days after topical application. Moreover, the transepidermal water loss rate (TEWL), stratum corneum (SC) hydration, melanin index (MI), erythema index (EI) and dendritic cells and inflammatory cell infiltration were noninvasively detected by the tester. LAST were performed before applying, 14 and 28 days after application, and then the test results were compared.Results:In this study, a total of 34 people participated in the test. The results of human skin enclosed patch test indicated that only 1 case of grade 1 reaction occurred among the tested subjects. The subjects felt good after applying the products, and the gel showed high degree of skin comfortable, no irritation and good tolerability. Subjective safety evaluation illustrated that the scores of pruritus, tingling and burning of the subjects decreased in D14 and D28 patient revisit, showing statistically significant differences (P < .05). When the gel was applied for 4 weeks, TEWL (8.42 ± 1.12) and EI (201.35 ± 13.51) were lower than the results before application (P < .05), and the SC hydration (65.36 ± 2.56) was higher than that before application (P < .05). There was no correlation between the SC hydration and TEWL (R = 0.092, P = .416). The results of skin CT indicated that the number of facial dendritic cells decreased in 17 subjects (accounting for 50%) in D28 patient revisit, and the degree of inflammatory cell infiltration decreased in 27 subjects (accounting for 80%). Compared with the baseline value, the LAST score and total sensory score decreased after application the product for 4 weeks, and the difference was statistically significant (the mean value of P < .05).Conclusion:The subjective feeling of application and efficacy of cosmetics in people with sensitive skin could be successfully evaluated by the comprehensive application of human skin enclosed patch test, long-term trial test, subjective safety evaluation and objective efficacy evaluation. And it provides the basis to judge whether the cosmetic is consistent with the efficacy claim of sensitive skin.     

Gastric cancer: Epidemiology,risk factors and prevention strategies

Gastric cancer(GC) is a global health problem, with more than 1 million people newly diagnosed with GC worldwide each year. GC is more prevalent in less developed countries than in more developed countries. About half of all GC cases worldwide occur in East Asia, notably China. Globally, overall incidence rates of GC are declining, which is potentially attributed to a decrease in Helicobacter pylori(H. pylori) infection and the use of refrigeration to preserve foods rather than salt. GC is a mul...     

miRNA-155对调节性T细胞表型和功能的影响

目的 探讨微小核糖核酸(miRNA)-155对调节性T细胞(Treg)的两种亚型诱导性Treg(iTreg)和天然性Treg(nTreg)的影响。 方法 采用健康成人外周血分离获取的外周血单个核细胞(PBMC),利用磁性细胞分选法分别获取幼稚T细胞和nTreg。培养阶段将细胞分为3组:对照组(幼稚T细胞加入白细胞介素-2培养)、iTreg组(幼稚T细胞加入白细胞介素-2和转化生长因子-β培养)和nTreg组(nTreg加入白细胞介素-2培养)。每组再分为3个亚组:未处理亚组、scramble亚组和miRNA-155拮抗剂亚组(每亚组3个孔)。采用低密度芯片分析方法检测3组中的未处理亚组细胞中miRNA-155的基因表达水平。采用流式细胞术检测3组中各亚组细胞的表面标志物CD25、Foxp3、CD127水平。采用流式细胞术检测3组中各亚组细胞的CD4⁺ CD25⁺Foxp3⁺SOCS1⁺ Treg比例;采用流式细胞术检测3组中各亚组细胞的Treg抑制功能。 结果 与对照组和iTreg组比较,nTreg组细胞的miRNA-155表达水平明显降低,差异有统计学意义(均为P<0.05)。与对照组和iTreg组比较,nTreg组的SOCS1表达水平明显升高,差异均有统计学意义(均为P<0.05)。加入miRNA-155拮抗剂后并未导致Foxp3、CD127和CD25等Treg重要表面标志物发生明显的变化。与对照组和iTreg组比较,nTreg组的SOCS1表达水平明显升高,差异均有统计学意义(均为P<0.05)。iTreg组中的未处理亚组细胞miRNA-155表达水平较低,而拮抗剂抑制其表达之后(miRNA-155拮抗剂亚组),能够使其SOCS1表达升高。iTreg组中,与未处理亚组比较,miRNA-155拮抗剂亚组的Treg抑制功能在1:8、1:16、1:32的比例时,显示出了更强的抑制功能(均为P<0.05)。 结论 体外拮抗miRNA-155对nTreg的抑制功能无明显影响,但是能够增加iTreg的SOCS1表达水平和体外抑制功能。     

肝移植术后胆泥超声声像图表现及其与预后相关性的初步探讨

目的  探讨肝移植术后胆泥超声表现及其与预后的关系。方法  回顾性分析36例肝移植术后胆泥患者的超声声像图表现, 按治疗结果分为不良疗效组和疗效稳定组, 比较两组间初次发现胆泥时的超声特点。结果  缺血性胆道病变合并胆泥者在不良疗效组中占95%(19/20), 在疗效稳定组中则仅为13%(2/16), 差异有统计学意义(P < 0.05)。疗效稳定组的胆泥均位于肝门部(16/16), 胆泥与胆管壁分界清晰(14/16), 肝门部胆管壁无增厚(14/16), 肝内胆管未见扩张(14/16);不良疗效组胆泥分布范围广, 除肝门部外, 常累及肝内胆管(10/20), 胆泥与胆管壁分界模糊(15/20), 肝门部胆管壁增厚明显(16/20), 肝内胆管扩张(19/20);上述4项指标在两组间差异有统计学意义(均为P < 0.05)。结论  在不同预后组间胆泥的声像图特点有差异, 对合并有缺血性胆道病变超声特点的胆泥, 提示预后较差, 可能需要进行积极的介入治疗。     

北京市门头沟区2018-2020年急性胃肠炎患者诺如病毒基因型分析

目的 了解北京市门头沟区急性胃肠炎患者诺如病毒基因型.方法 采用实时荧光定量PCR方法对急性胃肠炎患者粪便标本进行诺如病毒核酸检测,普通RT-PCR法对诺如病毒GⅡ基因群标本的多聚酶和衣壳蛋白区域扩增,对扩增产物序列测定.结果 粪便标本诺如病毒阳性检出率为10.75％,其中GⅡ基因群占95.89％,GI基因群占4.11...     

基于电子病历的临床决策支持研究可视化分析

目的/意义 探讨基于电子病历的临床决策支持领域研究现状、研究热点与前沿。方法/过程 基于文献计量方法,运用CiteSpace 6.2.R2软件绘制国家/地区分布、作者合作、机构合作、关键词共现和聚类科学知识图谱进行可视化分析,通过Python进行聚类热度挖掘与分析。结果/结论 基于电子病历数据的临床决策支持领域呈现快速发展态势,美国、中国为主要研究国家,国内外机构之间存在较强合作关系,关键词主要涉及电子病历、人工智能等。