乙肝患者血清总胆汁酸、前白蛋白与相关酶学指标联合检测的临床价值分析

目的检测乙型肝炎（HB）患者血清总胆汁酸（totalbileacide,TBA）、前白蛋白（prealbumin,PA）及肝功能酶学指标[γ谷氨酰转肽酶（γ-glutamyltranspetidase,GGT）、丙氨酸氨基转移酶（glutamic-pyruvic transaminase,ALT）、天门冬氨酸氨基转移酶（Aminotransferase,AST）、碱性磷酸酶（Alkaline phosphatase,ALP）]水平变化规律,探索TBA、PA与肝功能酶学指标联检在HB实验诊断中的临床价值。方法选择门诊和住院乙型病毒性肝病患者124例,正常对照组50例,用全自动生化分析仪测定血清TBA、PA与肝功能各酶学指标含量。结果各组TBA及GGT、ALT、AST、ALP水平高于健康对照组,差异有统计学意义（P〈0.05）。而PA、CHE均较正常对照组降低,差异有统计学意义（P〈0.01）。相关性分析显示,TBA、PA分别与CHE、GGT、ALT、AST、ALP水平呈正相关,差异有统计学意义（P〈0.05）。结论血清TBA、PA及肝功能酶学指标联检对肝病的临床诊断、鉴别诊断、病情判断有重要的临床参考价值。     

Alkalic dextranase produced by marine bacterium Cellulosimicrobium sp. PX02 and its application

The enzyme dextranase is widely used in the sugar and food industries, as well as in the medical field. Most land-derived dextranases are produced by fungi and have the disadvantages of long production cycles, low tolerance to environmental conditions, and low safety. The use of marine bacteria to produce dextranases may overcome these problems. In this study, a dextranase-producing bacterium was isolated from the Rizhao seacoast of Shandong, China. The bacterium, denoted as PX02, was identified as Cellulosimicrobium sp. and its growing conditions and the production and properties of its dextranase were investigated. The dextranase had a molecular weight of approximately 40 kDa, maximum activity at 40°C and pH 7.5, with a stability range of up to 45°C and pH 7.0–9.0. High-performance liquid chromatography showed that the dextranase hydrolyzed dextranT20 to isomaltotriose, maltopentaose, and isomaltooligosaccharides. Hydrolysis by dextranase produced excellent antioxidant effects, suggesting its potential use in the health food industry. Investigation of the action of the dextranase on Streptococcus mutans biofilm and scanning electron microscopy showed that it to be effective both for removing and inhibiting the formation of biofilms, suggesting its potential application in the dental industry.     

Complete nucleotide sequence of a Chinese isolate of Grapevine leafroll-associated virus 3 reveals a 5′ UTR of 802 nucleotides

Grapevine leafroll-associated virus 3 (GLRaV-3) is widely spread in China. Here we report, for the first time, the complete nucleotide sequence of the Chinese isolate (LN) of GLRaV-3. The 18,563-nt genomic RNA is the largest of the GLRaV-3 genomes reported to date, with a 5′ untranslated region of 802 nt. Its sequence shares 87.99–98.15 % identity with those of previously reported isolates, and phylogenetic analysis suggested placing isolate LN in group 3, together with another fully sequenced isolate, PL-20.