薄层扫描法测定食物中胆固醇的含量

本文探讨食物中胆固醇的薄层光密度法的测定。用硅胶Ｇ与０．３％ＣＭＣ－Ｎａ按１：２．２比例制成薄层板，石油醚－乙醚－－冰醋酸（７：３：０．１）为展开剂，５％磷钼酸乙醇溶液显色，日本岛津ＣＳ－９１０型薄层扫描仪定量扫描，测得精神异系数（ＲＤＳ％）为２．９２。稳定性变异系数（ＲＳＤ％）为２．３５，加嘏率在８７．９５－９４．２９％范围内。与气相色谱法（其回收率在９２－１０３％范围）比较，结果相接近，该法简     

日本血吸虫病患者幽门螺杆菌感染分析

目的：探讨日本血吸虫病患者中Hp的感染状况。方法：利用Hp全菌抗原ELISA定量检测不同类型日本血吸虫病患者血清抗HpIgG。结果：日本血吸虫病患者组血清抗HpIgG检出率（75％）明显高于对照组（46％），平均抗体水平（2.30±0.42）亦高于对照组（2.02±0.38），抗HpIgG的检出率和平均抗体水平随病程的发展而升高，血清抗HpIgG水平与血清抗AWA抗体水平高度相关（r＝0.92）。结论：日本血吸虫病患者的Hp感染率明显高于其他人，早期诊断并及时治疗Hp感染，对于控制晚期血吸虫病病变的发展和改善其预后具有重要意义。     

全肝容积灌注CT成像评估兔肝血流灌注及体积对肿瘤坏死率的影响

目的 探讨全肝容积灌注CT成像（VPCT）评估兔肝血流灌注及体积对肿瘤坏死率的影响。方法 采用兔肝VX2移植瘤作为实验组,并设立对照组。实验组及假手术对照组在术后14 d通过全肝VPCT并获得相应的灌注参数,测量并计算实验组肿瘤体积（TV）、肿瘤坏死率（TNR）。检查完后对所有实验组肿瘤标本进行病理学观察。结果 实验组癌组织与对照组肝组织灌注血流量（BF）、灌注血容量（BV）、动脉灌注量（ALP）、门静脉灌注量（PVP）、动脉灌注指数（HPI）比较,差异均有统计学意义（P <0.05）,实验组癌旁组织与对照组肝组织BF、BV、ALP、PVP、HPI比较,差异均无统计学意义（P >0.05）。Pearson相关性分析显示,TNR与ALP、HPI呈负相关（r =-0.410和-0.356,均P <0.05）,TNR与BF、BV、PVP无相关性（r =-0.016、-0.155和0.256,均P >0.05）;TV与BF、BV、PVP、ALP、HPI无相关性（r =0.062、0.292、0.140、0.142和0.014,均P >0.05）;肝VX2移植瘤TNR与TV无相关性（r =-0.098,P >0.05）。HE染色发现肿瘤边缘区为富含血管的环形癌组织,中央为坏死区。结论 兔肝VX2移植瘤TNR与全肝VPCT反映的肿瘤动脉灌注高低有关,与TV无明显关联。     

靶向敲减KLF4基因的重组腺相关病毒载体的构建及其在肺动脉高压动物模型中的应用

目的 构建靶向敲减KLF4基因的重组腺相关病毒载体,并应用于肺血管疾病,为后期研究肺血管KLF4基因敲减在肺动脉高压大鼠中的作用及相关分子机制奠定基础。方法 根据大鼠KLF4基因序列,设计针对大鼠特异性的siRNA序列,以pHBAAV-U6-MCS-CMV-EGFP作为空白载体,通过酶切技术构建带有GFP荧光标记的KLF4干扰腺病毒载体pHBAAV-r-KLF4 shRNA-GFP,行测序分析,并进行病毒扩增、纯化及滴度测定。本研究对长时间（3个月）接触香烟烟雾的大鼠进行气道注入AAV1-KLF4-shRNA的干预治疗,观察大鼠右心室收缩压、平均右心室压等血流动力学指标改变。结果 经测序检测显示,大鼠AAV1-KLF4-shRNA腺相关病毒载体构建成功。健康对照组、生理盐水模型组、对照病毒模型组、治疗干预组的右心室收缩压和平均右心室压比较,差异均有统计学意义（P <0.05）。结论 对长时间接触香烟烟雾的肺动脉高压模型大鼠应用AAV1-KLF4-shRNA腺相关病毒载体,能有效改善右心室收缩压和平均右心室压,该载体在相关疾病动物模型中应用有效,为后期顺利开展AAV1-KLF4-s...     

The effect of intrauterine devices (stainless steel ring, copper-T220 and levonorgestrel) on the DNA content in isolated human endometrial cells

The effect of three different intrauterine devices on the DNA content in human endometrial cells has been studied in 29 women before and after the insertion of an IUD; a stainless steel ring (9 subjects), Copper-T220 (11 subjects) and a medicated IUD releasing levonorgestrel at a rate of 2 micrograms/day (9 subjects). The material was grouped into "bleeders" (having more than 8 days of bleeding and spotting per month) and "non-bleeders" (having less than 8 days of bleeding and spotting per month). The DNA content was assessed on isolated endometrial cells smeared from thin biopsies and the amount of DNA per cell nucleus was measured by means of a Feulgen microspectrophotometric method. No significant difference was found between the "bleeders" and the "non-bleeders" as to the DNA content. Nevertheless, when the total number of subjects were pooled together, a significant increase in the DNA content was found in the post-insertion specimens in the groups of women using the SS and the T-Cu IUDs. Furthermore, a significant decrease in the DNA content was also found when the specimens obtained after the insertion of the LNG IUD were compared with those obtained after 24 months' use of the stainless steel ring and the Copper-T220 device (p less than 0.01 and p less than 0.002, respectively). It is concluded that the insertion of an inert IUD or an T-Cu IUD, induces significant alterations in the DNA content of the human endometrium adjacent to the device, whereas the use of the LNG device does not significantly modify the DNA synthesis when used for 3-10 months. No correlation was found between the DNA content per cell and the number of days of bleeding and spotting.     

五味与毒性关系的探讨

毒性的产生应有物质基础,这种物质基础就是五味,中药的功效和毒性均来自五味。在五味中有毒药物的数量以辛、苦味为多,甘味最少,酸、咸味居中。     

Attachment and spreading of fibroblasts on an RGD peptide-modified injectable hyaluronan hydrogel

Hyaluronan (HA) hydrogels resist attachment and spreading of fibroblasts and most other mammalian cell types. A thiol-modified HA (3,3'-dithiobis(propanoic dihydrazide) [HA-DTPH]) was modified with peptides containing the Arg-Gly-Asp (RGD) sequence and then crosslinked with polyethylene glycol (PEG) diacrylate (PEGDA) to create a biomaterial that supported cell attachment, spreading, and proliferation. The hydrogels were evaluated in vitro and in vivo in three assay systems. First, the behavior of human and murine fibroblasts on the surface of the hydrogels was evaluated. The concentration and structure of the RGD peptides and the length of the PEG spacer influenced cell attachment and spreading. Second, murine fibroblasts were seeded into HA-DTPH solutions and encapsulated via in situ crosslinking with or without bound RGD peptides. Cells remained viable and proliferated within the hydrogel for 15 days in vitro. Although the RGD peptides significantly enhanced cell proliferation on the hydrogel surface, the cell proliferation inside the hydrogel in vitro was increased only modestly. Third, HA-DTPH/PEGDA/peptide hydrogels were evaluated as injectable tissue engineering materials in vivo. A suspension of murine fibroblasts in HA-DTPH was crosslinked using PEGDA plus PEGDA peptide, and the viscous, gelling mixture was injected subcutaneously into the flanks of nude mice; gels formed in vivo following injection. After 4 weeks, growth of new fibrous tissue had been accelerated by the sense RGD peptides. Thus, attachment, spreading, and proliferation of cells is dramatically enhanced on RGD-modified surfaces but only modestly accelerated in vivo tissue formation.     

细胞外基质成分及其构筑对平滑肌细胞黏着斑激酶表达的影响

目的:探讨细胞外基质纤维连接蛋白(FN)、层黏连蛋白(LN)及三维基膜Matrigel胶对平滑肌细胞黏着斑激酶(FAK)表达的影响。方法:免疫荧光组织化学技术对生长在FN、LN和Matrigel胶里的平滑肌细胞FAK的表达进行检测。结果:生长存FN、LN和Matrigel胶里的平滑肌细胞FAK的表达依次为最高、次之和最低,各组之间有统计学意义;平滑肌细胞FAK磷酸化水平和FAK的表达呈正相关,生长在FN上的平滑肌细胞FAK(py397)的表达水平最高,生长在Matrigel胶里的平滑肌表达最低。结论:细胞外基质成分及其构筑对平滑肌细胞FAK的表达具有重要调节作用。     

超声成像的相位偏差校正

由于人体组织的各向异性，超声成像中存在相位偏差。相差制约了超声成像分辨率的进一步提高。互相关及其类似相位估计技术可以用于相差校正，jitter是此类算法的基本限制。并行自适应接收补偿算法可以校正较小的相差，结合互相关技术能够得到比较理想的相差校正效果。     

采用高脂饲料加空气干燥术建立颈动脉粥样硬化动物模型

目的:利用高脂饮食加空气干燥术建立一种稳定、重复性好、有较典型动脉粥样硬化病理改变的动物模型。方法:32只日本大耳白兔随机分为模型组(n=24)、对照组(n=8)。模型组给予高脂饲料喂养加空气干燥术,术中结扎左侧颈动脉分支血管,对照组正常饲料喂养,分别于术后第2、4、8、12周处死动物。取颈动脉组织切片HE染色,光镜下观察。结果:(1)75%兔颈总动脉存在细小血管分支,暂时结扎侧支血管后干燥效果更好。(2)对双侧颈动脉实施手术,结扎左侧分支,成模率更高。部分斑块显示出不稳定性。结论:采用改进后高脂饮食加空气干燥术可成功建立兔颈动脉粥样硬化模型,其病理特点适合于目前临床研究。