IL-6、IL-1及TNF对人类骨髓瘤细胞系KM_2、KM_3增殖的影响

细胞因子的异常表达对骨髓瘤细胞的恶性增殖起重要作用。本文研究了IL-6、IL-1及TNF 对人骨髓瘤细胞系增殖的影响。KM_2、KM,均能分泌IL-6,以维持自身的增殖。培养体系中加入抗IL-6抗体可抑制瘤细胞的增殖,这种抑制作用可通过加入重组IL-6而逆转.在培养体系中加入重组IL-6和TNF 均可促进KM_2、KM_3的增殖,而IL-1无此作用。培养体系中加入TNF 培养后,上清中IL-6活性增高。我们的结果提示,人骨髓瘤细胞系KM_2、KM_3存在IL-6自分泌增殖机制,而TNF 可加强这种自分泌作用。     

红细胞聚集与取向对其悬浮液电阻抗的影响——I.实验研究

本文通过测量具有不同流变特性的红细胞悬浮液电阻抗的动态与静态变化，研究了红细胞聚集与取向对其悬浮液电阻抗的影响。首次得出以下结论：１．红细胞聚集使其悬浮液电阻抗显著减小。２．由于低切变率下红细胞聚集与高切变率下红细胞取向的共同影响，使血液电阻抗随切变率的变化具有双相特性。这增加了对血液电特性的新认识，表明可利用电阻抗测定红细胞聚性。     

吗啡增强备用根大鼠脊髓后角组织提取液对培养鸡胚背根节的促神经突起生长的作用

用组织培养方法，探讨备用很大鼠手术侧和非手术侧脊髓后角组织提取液对鸡胚背根节（ＤＲＧ）神经突起的促生长作用，以及应用吗啡对此作用的影响。结果显示：备用根大鼠手术侧脊髓后角组织提取液作用的ＤＲＧ神经突起密度（３６．４２±４．６９），比非手术侧的（２３．９６±３．４７）明显增大。提示去初级传入纤维的脊髓后角组织具有促进ＤＲＧ神经突起生长的神经营养活性作用。应用吗啡的备用根大鼠手术侧脊髓后角组织提取液作用的ＤＲＧ神经突起密度（６４．１９±９．２４），又比备用根大鼠手术侧的大。这表明，吗啡具有进一步增强去初级传入纤维支配的脊髓后角组织提取液促进培养的ＤＲＧ神经突起生长的效应。     

Isolation of Nanjing local strains of HHV-7 and their biological and immunological characteristics

To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,fourstrains of herpesvirus were isolated from saliva specimens of one healthy individual and three childrensuffering from a kidney disease in Nanjing.The viruses were identified by transmission electron microscopy(EM),indirect immunofluorescence assay(IFA) with a specific monoclonal antibody;nested polymerase chainreaction,restriction mapping and DNA sequencing.The virus-infected cells showed the typical cytophathiceffect(CPE) under microscopy and could be detected by IFA with the human herpesvirus-7(HHV-7) specificantibody.Under EM,herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm.HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mappingand DNA sequencing.Similarly to DC strain,an known HHV-7 strain used in the present study as the positivecontrol,the virus could be inactivated by ultraviolet irradiation for 10 min,heated at 45℃ for 30 min,pH<5or>9 at 4℃ for 2 h and ether or chloroform for 10 h.The virus induced the production of TNF-α,IL-10 andIL-12p70 while inhibited IFN-γ secretion,increased the percentage of CD2~+ cells while decreased that of CD4~+or CD45RA~+ cells.The results indicate that the viruses isolated in Nanjing are HHV-7,which has similarbiological characteristic to the known HHV-7 strain,DC.Infection with HHV-7 in vitro could affect immunefunction of lymphocytes by disturbing cytokine production and CD antigen expression.Cellular & MolecularImmunology.2004;1(5):367-372.     

知识模式转换在医学科技创新中的作用

医学科技创新是医院赖以生存与发展的动力,医学科技创新的过程也是医学知识模式不断转换的过程,本文从知识模式转换的视角,探讨知识管理对医学科技创新的意义与作用     

肘内侧入路的临床应用解剖及其意义

目的:为肘内侧入路手术避免损伤重要结构提供解剖学基础。方法:选教学用的固定尸体标本24具(男18女6)48侧,按手术入路层次,对肘内侧入路的相关血管神经进行解剖学观测。结果:(1)臂内侧皮神经于臂中部的内侧面浅出,直径为(1.05±0.35)mm;前臂内侧皮神经于臂内侧中下1/3肱二头肌内侧沟伴贵要静脉浅出,直径为(1.50±0.55)mm。(2)尺神经干在肘部发出1~3肘关节支、1~4尺侧腕屈肌支和1~4指深屈肌支。(3)尺侧上副动脉、尺侧下副动脉和尺侧返动脉后支从肱动脉的起点处至尺神经垂直距离分别为(1.65±0.35)cm、(2.43±0.54)cm、(1.86±0.41)cm;与尺神经伴行至内上髁的距离分别为(14.38±1.82)cm、(4.51±1.16)cm、(5.91±0.67)cm。肘部附近尺侧下副动脉和尺侧返动脉后支与尺侧上副动脉在尺神经外膜相吻合。结论:(1)肘内侧入路浅层必须寻找和保护臂内侧皮神经和前臂内侧皮神经;(2)肘内侧入路保护尺神经血供及其肌支是临床手术成败的关键。     

桥粒斑蛋白II在肿瘤组织中的表达

桥粒斑蛋白ＩＩ（ＤＰＩＩ）是一种新的上皮和上皮性肿瘤标记物。我们应用针对石蜡切片的抗ＤＰＩＩ单抗和抗细胞角蛋白（ＣＫ）单抗对２ｌ７例肿瘤作了免疫组织化学ＡＢＣ法染色的研究，结果：在１６３例上皮性肿瘤和双向分化的肿瘤中，１４７例表达ＤＰＩＩ，占９０．７％，９２例表达ＣＫ，占５６．４％。ＣＫ阳性染色全部位于细胞浆内，ＤＰＩＩ阳性染色则同时分布在细胞交界面上和细胞浆中。其他５４例不含桥粒的肿瘤均无ＤＰＩＩ和ＣＫ表达。结果表明，ＤＰＩＩ单抗是有用的含桥粒肿瘤的免疫组化探针。因ＤＰＩＩ不存在于一切含桥粒肿瘤，故在鉴别上皮性肿瘤时，ＤＰＩＩ单抗尚需与其他上皮标记物的抗体合并使用。     

湖南侗族指纹白线正常值分析

目的 :分析侗族指纹白线的出现率 ,并探讨其相关因素。材料与方法 :用油墨拓印法印取指纹图 ,按Schouman方法确定指纹白线 ,并对获取的资料进行统计分析。结果 :(1 )湖南侗族指纹白线出现率为 3 7 2 7% ,女性明显高于男性 (P <0 .0 0 1 ) ;(2 )侗族左手指纹白线出现率明显高于右手 (P <0 .0 0 1 ) ;(3 )侗族AB血型人指纹白线出现率低于其他血型人群 (P <0 .0 5 ) ;(4 )指纹白线在各手指的出现率按中指 >环指 >示指 >拇指 >小指的顺序排列。结论 :湖南侗族指纹白线出现率高于我国其他几个民族 ,其出现率与性别、手别、指别、血型有关     

术苓健脾胶囊对TNBS诱导的大鼠实验性结肠炎的作用及机制

探讨术苓健脾胶囊（术苓）对2,4,6-三硝基苯磺酸（TNBS）诱导的大鼠实验性结肠炎的作用及其机制。采用2.5%TNBS灌肠制备大鼠实验性结肠炎模型。将大鼠随机分为正常组、模型组、肠炎宁（180 mg/kg）组以及术苓低剂量（40 mg/kg）、高剂量（120 mg/kg）组。造模后药物治疗7 d后处死大鼠。其间每日记录大鼠体重,观察大鼠疾病活动状态。实验结束后,取结肠组织进行HE病理学分析、检测结肠组织中髓过氧化物酶（MPO）酶活、诱导型一氧化氮合酶（iNOS）、炎性细胞因子（IL-6、IL-1β、IFN-γ、IL-10）及紧密连接蛋白（occludin、ZO-1）的表达水平,并检测血清中炎性细胞因子（IL-6、IL-1β）的含量。结果表明,与模型组相比,术苓给药显著缓解了TNBS造模引起的体重下降、疾病活动指数（DAI）升高,缓解了结肠组织的缩短、水肿以及病理学损伤,减少了炎性细胞浸润、隐窝的破坏与杯状细胞的丢失,降低了结肠组织MPO酶活性、iNOS与炎性细胞因子的水平,增加了结肠紧密连接蛋白的表达,同时降低了血清中的炎性因子的含量。结果表明,术苓通过降低炎性因子水平,增加肠道紧密...     

Post-translational modification of proteins by arginine and lysine following crush injury and during regeneration of rat sciatic nerves

Following crush injury to rat sciatic nerves, a crude fraction of the 150,000 g supernatant can post-translationally incorporate [3H]Arg and [3H]Lys into endogenous proteins in amounts approximately 10 times uninjured control nerves. These increases occur in the proximal nerve stump within 2 h of injury and 2 weeks later in a distal segment of nerve containing the tips of the regenerating axons. In the present experiments, the endogenous nerve proteins modified by Arg or Lys in these nerve segments have been identified using two-dimensional polyacrylamide gel electrophoresis. The fraction used to assay for protein modification, the void volume of a Sephacryl S-300 column, was found to contain only a few proteins visible by Coomassie blue staining, one of which is likely to be albumin (68 kDa, pI 6.4). While this protein was modified by both Arg and Lys, the majority of label was found in areas not showing Coomassie blue staining. This indicates that of the many potential targets of post-translational arginylation and lysylation, most are proteins of relatively low abundance. A variety of proteins were modified by Arg or Lys alone while others were modified by both Arg and Lys. A high molecular weight protein (175 kDa, pI 9.0) was modified only by Lys and only at 2 h post crush. Of a variety of modified proteins of approximately 17 kDa one (pI 6.3) was modified by both Arg and Lys and at both time points, while another (pI 9.0) was modified at both time points, but only by Lys. The results show that Arg and Lys can be added post-translationally to a large number of low abundance, soluble sciatic nerve proteins, and that some of those proteins are modified only by Arg or Lys while others are modified by both Arg and Lys. Also, the modification of certain proteins appears to be associated specifically with the immediate response of a nerve to injury (e.g. 88 kDa, pI 7.1) while others are associated with the regenerative period (e.g. 56 kDa, pI 7.4).