全文获取类型
收费全文 | 27073篇 |
免费 | 1440篇 |
国内免费 | 152篇 |
专业分类
耳鼻咽喉 | 279篇 |
儿科学 | 566篇 |
妇产科学 | 623篇 |
基础医学 | 3473篇 |
口腔科学 | 1042篇 |
临床医学 | 1955篇 |
内科学 | 7213篇 |
皮肤病学 | 706篇 |
神经病学 | 2098篇 |
特种医学 | 561篇 |
外科学 | 4448篇 |
综合类 | 176篇 |
一般理论 | 3篇 |
预防医学 | 1944篇 |
眼科学 | 452篇 |
药学 | 1478篇 |
中国医学 | 125篇 |
肿瘤学 | 1523篇 |
出版年
2023年 | 188篇 |
2022年 | 373篇 |
2021年 | 960篇 |
2020年 | 442篇 |
2019年 | 899篇 |
2018年 | 1040篇 |
2017年 | 582篇 |
2016年 | 590篇 |
2015年 | 763篇 |
2014年 | 1118篇 |
2013年 | 1394篇 |
2012年 | 2204篇 |
2011年 | 2279篇 |
2010年 | 1335篇 |
2009年 | 1164篇 |
2008年 | 1837篇 |
2007年 | 1892篇 |
2006年 | 1734篇 |
2005年 | 1548篇 |
2004年 | 1438篇 |
2003年 | 1370篇 |
2002年 | 1261篇 |
2001年 | 175篇 |
2000年 | 157篇 |
1999年 | 200篇 |
1998年 | 195篇 |
1997年 | 151篇 |
1996年 | 150篇 |
1995年 | 130篇 |
1994年 | 104篇 |
1993年 | 86篇 |
1992年 | 81篇 |
1991年 | 60篇 |
1990年 | 56篇 |
1989年 | 45篇 |
1988年 | 47篇 |
1987年 | 34篇 |
1986年 | 24篇 |
1985年 | 42篇 |
1984年 | 37篇 |
1983年 | 42篇 |
1982年 | 50篇 |
1981年 | 48篇 |
1980年 | 46篇 |
1979年 | 23篇 |
1978年 | 27篇 |
1977年 | 31篇 |
1976年 | 27篇 |
1975年 | 28篇 |
1974年 | 26篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
51.
Axotomized neonatal motoneurons overexpressing the bcl2 proto-oncogene retain functional electrophysiological properties. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S Alberi M Raggenbass F de Bilbao M Dubois-Dauphin 《Proceedings of the National Academy of Sciences of the United States of America》1996,93(9):3978-3983
Bcl2 overexpression prevents axotomy-induced neuronal death of neonatal facial motoneurons, as defined by morphological criteria. However, the functional properties of these surviving lesioned transgenic neurons are unknown. Using transgenic mice overexpressing the protein Bcl2, we have investigated the bioelectrical properties of transgenic facial motoneurons from 7 to 20 days after neonatal unilateral axotomy using brain-stem slices and whole cell patch-clamp recording. Nonaxotomized facial motoneurons from wild-type and transgenic mice had similar properties; they had an input resistance of 38 +/- 6 M omega and fired repetitively after injection of positive current pulses. When cells were voltage-clamped at or near their resting membrane potential, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), N-methyl-D-aspartic acid (NMDA), or vasopressin generated sustained inward currents. In transgenic axotomized mice, facial motoneurons could be found located ipsilaterally to the lesion; they had an input resistance of 150 +/- 30 M omega, indicating that they were smaller in size, fired repetitively, and were also responsive to AMPA, NMDA, and vasopressin. Morphological measurements achieved 1 week after the lesion have shown that application of brain-derived neurotrophic factor prevented the reduction in size of axotomized transgenic motoneurons. These data indicate that Bcl2 not only prevents morphological apoptotic death of axotomized neonatal transgenic motoneurons but also permits motoneurons to conserve functional electrophysiological properties. 相似文献
52.
Luis Chávez de Paz Gunnel Svens?ter Gunnar Dahlén Gunnar Bergenholtz 《Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics》2005,100(2):232-241
OBJECTIVES: The object of this study was to investigate the diversity among streptococcal species isolated from root canals in conjunction with endodontic therapy and to characterize their production of extracellular proteins. STUDY DESIGN: Consecutive root canal samples (RCS) taken as bacteriological controls during root canal treatment of teeth with apical periodontitis were analyzed in a total of 100 clinical cases. Bacteria were isolated and classified by selective media and gas liquid chromatography. Streptococcal strains were identified by carbohydrate fermentation, hydrolysis of aesculin/arginine, and production of enzymes. Releases of extracellular proteins by streptococci and Enterococcus spp in fluid culture media were examined with SDS-PAGE and 2-dimension gel electrophoresis (2 DE). Extracellular proteins produced were quantified and qualitatively analyzed. Specific proteins were targeted with Western immunoblot assays. Comparisons were made with type strains. RESULTS: Of a total of 241 bacterial strains recovered in the first samples submitted, Streptococcus gordonii, S anginosus, and S oralis were the most frequently isolated streptococci. In 49 of 89 resubmitted samples showing bacterial growth, S gordonii and S oralis still predominated among streptococci. Other common bacterial isolates were Enterococcus spp, Lactobacillus paracasei, and Olsenella uli. Quantitative and qualitative differences in extracellular protein production were observed among clinical isolates and laboratory streptococcal strains. In similar conditions for growth, S intermedius, S anginosus, S oralis, and S gordonii were strong producers of extracellular proteins (>3.0 microg/mL), while Enterococcus spp and S mutans were weak. Whole cell protein extracts showed a different profile from that of extracellular proteins. The chaperone protein DnaK was recognized to be produced extracellularly by S gordonii, S oralis, S anginosus, and S parasanguis. CONCLUSIONS: Being strong producers of extracellular proteins and by virtue of common presence in teeth undergoing endodontic therapy, S gordonii, S anginosus, and S oralis may be of pathogenic significance in posttreatment apical periodontitis. 相似文献
53.
54.
Luis Hernandez-Garcia Gregory R Lee Alberto L Vazquez Chun-Yu Yip Douglas C Noll 《Magnetic resonance in medicine》2005,54(4):955-964
A new approach to modeling the signal observed in arterial spin labeling (ASL) experiments during changing perfusion conditions is presented in this article. The new model uses numerical methods to extend first-order kinetic principles to include the changes in arrival time of the arterial tag that occur during neuronal activation. Estimation of the perfusion function from the ASL signal using this model is also demonstrated. The estimation algorithm uses a roughness penalty as well as prior information. The approach is demonstrated in numerical simulations and human experiments. The approach presented here is particularly suitable for fast ASL acquisition schemes, such as turbo continuous ASL (Turbo-CASL), which allows subtraction pairs to be acquired in less than 3 s but is sensitive to arrival time changes. This modeling approach can also be extended to other acquisition schemes. 相似文献
55.
José Ramón Cansino Alcaide Luis Martínez-Piñeiro 《Clinical & translational oncology》2006,8(3):148-152
Summary Genes involved in cancer generation are usually tumor suppressors and oncogenes. Progressive genetic alterations in these
genes are involved in the mechanisms of tumorigenesis. In prostate cancer, additionally several chromosomal loci that should
harbor mutated genes have been proposed. Some genes have been found altered in prostate cancer, such as PTEN, TP53, AR, RNASEL
(HPC1), ELAC2 (HPC2), CDKN2A and MSR1 and those can be natural targets for new strategies of treatment. Besides, gene therapy
has been suggested to be suitable for prostate cancer treatment. This approach includesex vivo corrective therapy, suicide, and antisense therapy. 相似文献
56.
Carlos Bada Nilton Yhuri Carreazo Juan Pablo Chalco Luis Huicho 《Revista paulista de medicina》2007,125(3):150-154
CONTEXT AND OBJECTIVE: Many children with acute lower respiratory tract infections (ALRI) present to the emergency ward with concurrent wheezing. A chest x-ray is often requested to rule out pneumonia. We assessed inter-observer agreement in interpreting x-rays on such children. DESIGNS AND SETTING: Prospective consecutive case study at Instituto de Salud del Ni?o, Lima, Peru. METHODS: Chest x-rays were obtained from eligible children younger than two years old with ALRI and concurrent wheezing who were seen in the emergency ward of a nationwide pediatric referral hospital. The x-rays were read independently by three different pediatric residents who were aware only that the children had a respiratory infection. All the children had received inhaled beta-adrenergic agonists before undergoing chest x-rays. Lobar and complicated pneumonia cases were excluded from the study. RESULTS: Two hundred x-rays were read. The overall kappa index was 0.2. The highest individual kappa values for specific x-ray findings ranged from 0.26 to 0.34 for rib horizontalization and from 0.14 to 0.31 for alveolar infiltrate. Inter-observer variation was intermediate for alveolar infiltrate (kappa 0.14 to 0.21) and for air bronchogram (kappa 0.13 to 0.23). Reinforcement of the bronchovascular network (kappa 0.10 to 0.16) and air trapping (kappa 0.05 to 0.20) had the lowest agreement. CONCLUSIONS: There was poor inter-observer agreement for chest x-ray interpretation on children with ALRI and concurrent wheezing seen at the emergency ward. This may preclude reliable diagnosing of pneumonia in settings where residents make management decisions regarding sick children. The effects of training on inter-observer variation need further studies. 相似文献
57.
M H Miller W S Tucker J M Bilbao 《Journal l'Association canadienne des radiologistes》1986,37(1):35-37
Multiple vascular lesions in the brain were identified by angiography in a 45-year-old woman with Von Hippel Lindau disease. One of these lesions was a histologically-proven hemangioblastoma. The occurrence of such lesions in a cerebral hemisphere is exceedingly rare and is usually related to Von Hippel Lindau disease. 相似文献
58.
59.
J Estev?o-Costa J Correia-Pinto A Fragoso M Soares-Oliveira J Luis Carvalho 《Zeitschrift für Kinderchirurgie》2003,13(5):344-346
Splenic necrosis is extremely rare in neonates, the cases recorded so far being secondary to torsion of wandering organs. A newborn with an abdominal mass who underwent exchange transfusions through an umbilical catheter is presented here. Comprehensive investigation led to the suspicion of enteric duplication, but a splenic necrosis with no features of wandering spleen was found at laparotomy. The pathogenesis and preoperative diagnostic work-up of splenic necrosis are emphasised. 相似文献
60.
Lidice Bernardo Adriana Yndart Susana Vzquez Luis Morier María G. Guzmn 《Clinical and Vaccine Immunology : CVI》2005,12(2):361-362
The possibility of a correlation between dengue virus genotype groups and disease severity is currently under discussion. The objective of this investigation was to identify any immunogenic difference between the American and Asian dengue 2 virus genotypes through the study of antibody development (virus-binding immunoglobulin G and neutralizing antibodies) in mice. Differences in the neutralization pattern between the strains studied were observed, suggesting the presence of slight antigenic variations among them. The lack of recognition of one of the Asian genotype strains was remarkable. 相似文献