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91.
Garcia TC Fonseca CT Pacifico LG Durães Fdo V Marinho FA Penido ML Caliari MV de Melo AL Pinto HA Barsante MM Cunha-Neto E Oliveira SC 《Acta tropica》2008,106(3):162-167
Sm14 and paramyosin are two major Schistosoma mansoni vaccine candidate antigens. Recently, we have identified Sm14 and paramyosin epitopes that are recognized by T cells of resistant individuals living in endemic areas for schistosomiasis. Herein, mice were immunized with these peptides separately or in association in order to evaluate their vaccine potential. Immunization of mice with Sm14 peptides alone or mixed with paramyosin peptides was able to induce 26%-36.7% or 28%-29.2% of worm burden reduction, 67% or 46% of intestinal eggs reduction and also 54%-61% or 43%-52% of liver pathology reduction, respectively. Protection was associated with a Th1 type of immune response induced by Sm14 peptide immunization. In contrast, paramyosin peptide vaccination did not engender protective immunity or liver pathology reduction and immunization was associated with a Th2 type of immune response. 相似文献
92.
Lucila M Perrotta de Souza Jessica PL Moreira Homero S Fogaça José Marcus Raso Eulálio Ronir R Luiz Heitor SP de Souza 《Hepatobiliary & pancreatic diseases international : HBPD INT》2019,18(1):79-86
Background
Currently, surgical resection represents the only curative treatment for pancreatic cancer (PC), however, the majority of tumors are no longer resectable by the time of diagnosis. The aim of this study was to describe time trends and distribution of pancreaticoduodenectomies (PDs) performed for treating PC in Brazil in recent years.Methods
Data were retrospectively obtained from Brazilian Health Public System (namely DATASUS) regarding hospitalizations for PC and PD in Brazil from January 2008 to December 2015. PC and PD rates and their mortalities were estimated from DATASUS hospitalizations and analyzed for age, gender and demographic characteristics.Results
A total of 2364 PDs were retrieved. Albeit PC incidence more than doubled, the number of PDs increased only 37%. Most PDs were performed in men (52.2%) and patients between 50 and 69 years old (59.5%). Patients not surgically treated and those 70 years or older had the highest in-hospital mortality rates. The most developed regions (Southeast and South) as well as large metropolitan integrated municipalities registered 76.2% and 54.8% of the procedures, respectively. LMIM PD mortality fluctuated, ranging from 13.6% in 2008 to 11.8% in 2015.Conclusions
This study suggests a trend towards regionalization and volume-outcome relationships for PD due to PC, as large metropolitan integrated municipalities registered most of the PDs and more stable mortality rates. The substantial differences between PD and PC increasing rates reveals a limiting step on the health system resoluteness. Reduction in the number of hospital beds and late access to hospitalization, despite improvement in diagnostic methods, could at least in part explain these findings. 相似文献93.
María Jezabel Haye Salinas Soledad Retamozo Ana Cecilia Alvarez Hernán Maldonado Ficco Fernando Dal Pra Gustavo Citera Mariana Benegas Rafael Chaparro del Moral Oscar Rillo Anastasia Secco Lucila Marino Claverie Antonio Catalan Pellet Josefina Marcos Mercedes Argentina García Juan Carlos Marcos Ana Barbaglia Verónica Bellomio Alberto Berman Cristian Quiroz Enrique R Soriano Federico Ceccato Sergio Paira Doralia Vazquez Vicente Ricardo Juarez Edson Javier Velozo Gabriela Salvatierra Francisco Caeiro 《Rheumatology international》2015,35(5):855-859
94.
Alessandra Timponi Goes Cruz Caroline Wichnieski Everdan Carneiro Ulisses Xavier da Silva Neto Gianluca Gambarini Lucila Piasecki 《Journal of endodontics》2017,43(10):1716-1719
Introduction
The purpose of this study was to evaluate in vitro the efficacy of both the electronic apex locator (EAL) and auto apical reverse (AAR) functions of the endodontic motor MM Control (Micro-Mega, Besançon Cedex, France) compared with Root ZX II (J Morita, Tokyo, Japan).Methods
After cervical preflaring, the actual lengths (ALs) of 36 single-rooted teeth were obtained up to the apical foramen. The EAL measurements at the marks “APEX” and “0.5” of both devices were obtained using an alginate model. The teeth were divided randomly into 2 groups (n = 18), and root canal preparation was performed with rotary instruments using the AAR function (0.5 mark) of each motor. The length provided by the AAR was compared with the visual length after preparation (AL2). The differences between the electronic lengths and the respective visual measurements were assigned as negative for lower or positive when higher. The means of the absolute values and the percentages of distribution of the electronic measurements between devices were compared.Results
There was no difference between the devices in terms of the means of the EAL measurements or AAR length (analysis of variance, P > .05). However, the EAL function of MM Control presented a greater percentage of measurements >1.01 mm longer than AL (chi-square, P < .01). The AAR function provided an acceptable apical limit in 83.3% of the cases for Root ZX and 77.8% of the cases for MM Control.Conclusions
The AAR function of both MM Control and Root ZX II provided an adequate apical limit of preparation in vitro. However, the use of only the EAL function of MM Control resulted in significantly more cases of overextended readings. 相似文献95.
Adham A. Azim Howard H. Wang Mohamed Tarrosh Katharina A. Azim Lucila Piasecki 《Journal of endodontics》2018,44(11):1720-1724
Introduction
The aim of this study was to evaluate in vitro the performance of 3 single-file systems: WaveOne Gold Primary (WOG; Dentsply Sirona, Ballaigues, Switzerland), Hyflex EDM “one file” (EDM; Coltene/Whaledent, Alstatten, Switzerland), and XP Shaper (XP; FKG, La Chaux de Fonds, Switzerland), for nonsurgical endodontic retreatment on mandibular anterior teeth in regard to the amount of remaining filling material inside the canal, debris extrusion, and operation time.Methods
Sixty extracted standardized mandibular incisors presenting with a single oval canal were prepared to size 30.04 and obturated using warm vertical condensation. After 30 days (37°C, 100% humidity), teeth were randomly divided into 3 groups (n = 20) according to the file used for retreatment (WOG, EDM, or XP operated at 3000 rpm). During the retreatment procedures, preweighed vials were used to collect apically extruded debris, and the time required to remove the filling material was recorded in seconds. Cone-beam computed tomographic scans were taken before and after the retreatment to determine the amount of remaining filling material inside the root canal space. Statistical analysis was performed using the Kruskal-Wallis test, 1-way analysis of variance, and the Pearson test at a 5% significance level.Results
There was a statistically significant difference among the groups regarding the instrumentation time (P < .05). XP was the fastest to remove gutta-percha from the canals (40.54 ± 28.03 seconds) followed by EDM (55.77 ± 19.76 seconds) and WOG (105.92 ± 53.92 seconds). The percentage of remaining filling was significantly greater for WOG (26.65% ± 14.63%) (P < .05). No difference was found between EDM (13.51% ± 9.53%) and XP (8.60% ± 7.28%). No difference was found regarding the amount of extruded debris among the 3 groups (P > .05).Conclusions
XP was the most efficient in gutta-percha removal from the canals when operated at a higher speed (3000 rpm) followed by EDM and WOG. 相似文献96.
Andrea Beatriz ACQUIER Alejandra Karina De Couto PITA Lucila BUSCH Gabriel Antonio SáNCHEZ 《Journal of applied oral science : revista FOB》2015,23(3):288-294
Objective
Salivary mucin and amylase levels are increased in patients with chronic periodontitis (CP). Due to the fact that aggressive periodontitis (AgP) not only differs from chronic periodontitis in terms of its clinical manifestation, the aim of this study was to compare salivary mucin and amylase levels and their relation to the clinical parameters of patients with aggressive periodontitis with that of patients with chronic periodontitis.Material and Methods
Eighty subjects were divided into two groups: 20 patients with AgP and their 20 matched controls and 20 patients with CP and their 20 matched controls, based on clinical attachment loss (CAL), probing pocket depth (PPD) and bleeding on probing (BOP). Whole unstimulated saliva was obtained and mucin, amylase and protein were determined by colorimetric methods. Pearson’s correlation analysis was used to determine the relationship between salivary mucin, amylase and protein levels and the clinical parameters.Results
Salivary mucin, amylase and protein levels were increased in patients with AgP and CP but there were no differences between them or between control groups. Pearson’s correlation analysis, determined in the entire subjects studied, showed a positive and significant correlation of mucin, amylase and proteins with CAL and PPD and a negative correlation with the flow rate. When Pearson’s correlation analysis was carried out in each group separately, Fisher’s z transformation showed no significant difference between both groups.Conclusion
Comparison of the salivary levels of mucin, amylase and protein and their relationship with clinical parameters of AgP patients with that of CP patients revealed no differences between both groups. 相似文献97.
Influence of ketone bodies on oxidative stress parameters in brain of developing rats in vitro 总被引:1,自引:0,他引:1
Beskow AP Fernandes CG Leipnitz G da Silva Lde B Seminotti B Amaral AU Wyse AT Wannmacher CM Vargas CR Dutra-Filho CS Wajner M 《Metabolic brain disease》2008,23(4):411-425
Pro-oxidant and antioxidant properties have been found for acetoacetate (AcAc) and β-hydroxybutyrate (BHB) in peripheral tissues.
In the present study we investigated the role of AcAc and BHB at concentrations found in diabetic patients during ketoacidotic
crises and in individuals affected by succinyl CoA: 3-oxoacid CoA transferase and acetoacetyl-CoA thiolase deficiencies, disorders
clinically characterized by neurological symptoms, on a large number of oxidative stress parameters in fresh cerebral cortex
of developing rats. Lipid peroxidation (chemiluminescence and thiobarbituric acid–reactive substances levels), protein oxidative
damage (carbonyl formation and sulfhydryl oxidation), 2′,7′-dichlorofluorescin diacetate oxidation and the non-enzymatic (total
antioxidant reactivity and glutathione levels) and enzymatic (glutathione peroxidase, superoxide dismutase and catalase activities)
antioxidant defenses were not changed by doses of BHB and AcAc as high as 25 mM in cortical supernatants under basal conditions.
Furthermore, BHB did not affect the increased thiobarbituric acid–reactive substances levels provoked by 3-hydroxy-3-methylglutaric
and 3-methylglutaconic acids and by a hydroxyl-induced generation system. Finally, BHB and AcAc were not able to oxidize sulfhydryl
groups from a commercial GSH solution. Therefore, under basal conditions or under situations with high production of free
radicals, AcAc and BHB were not able to reduce or increase the oxidative stress parameters in the brain. Taken together, our
present results do not support the hypothesis that BHB and AcAc act as potent direct or indirect pro-oxidants or antioxidants
in the CNS. 相似文献
98.
99.
Lucila Marino Claverie Elizabeth Knobel Lorena Takashima Lorena Techera Marina Oliver Paula Gonzalez Félix E. Romanini María L. Fonseca Marta N. Mamani 《Clinical rheumatology》2013,32(6):839-843
Changes in nailfold capillaroscopy in systemic sclerosis patients could be related to the disease severity. The aim of this study was to investigate whether patients with “late” scleroderma (SD) pattern have more organ involvement than patients with “early/active” SD pattern. Forty-six Argentinian patients (44 women and 2 men), with a diagnosis of systemic sclerosis, were distributed in two groups based on the presence of late and early/active patterns. Organ involvement was assessed as follows: pulmonary function by chest radiography, high-resolution chest tomography (HRCT), lung volume tests, and diffusing capacity for carbon monoxide (DLCO); esophageal involvement by manometry; and pulmonary arterial hypertension (PAH) by Doppler echocardiography and six-minute walk test. Honeycombing of the lungs evaluated by HRCT was more frequently present in patients with late pattern compared with early/active patients (p?=?0.01). We also found statistically significant differences in lung volume tests (p?=?0.03) and DLCO (p?=?0.02) between the two SD pattern groups. Esophageal manometry showed a significantly higher frequency of motility disorders in the group with late pattern (p?=?0.0024). In this study, patients with late pattern had higher frequency of pulmonary and esophageal involvement compared with patients with early/active pattern. 相似文献
100.
Lu D Garcia-Contreras L Xu D Kurtz SL Liu J Braunstein M McMurray DN Hickey AJ 《Pharmaceutical research》2007,24(10):1834-1843
PURPOSE: To investigate the use of poly (lactide-co-glycolide) (PLGA) microparticles in respirable sizes as carriers for Antigen 85B (Ag85B), a secreted protein of Mycobacterium tuberculosis, with the ultimate goal of employing them in pulmonary delivery of tuberculosis vaccine. MATERIALS AND METHODS: Recombinant Ag85B was expressed from two Escherichia coli strains and encapsulated by spray-drying in PLGA microspheres with/without adjuvants. These microspheres containing rAg85B were assessed for their ability to deliver antigen to macrophages for subsequent processing and presentation to the specific CD4 T-hybridoma cells DB-1. DB-1 cells recognize the Ag85B(97-112) epitope presented in the context of MHC class II and secrete IL-2 as the cytokine marker. RESULTS: Microspheres suitable for aerosol delivery to the lungs (3.4-4.3 microm median diameter) and targeting alveolar macrophages were manufactured. THP-1 macrophage-like cells exposed with PLGA-rAg85B microspheres induced the DB-1 cells to produce IL-2 at a level that was two orders of magnitude larger than the response elicited by soluble rAg85B. This formulation demonstrated extended epitope presentation. CONCLUSIONS: PLGA microspheres in respirable sizes were effective in delivering rAg85B in an immunologically relevant manner to macrophages. These results are a foundation for further investigation into the potential use of PLGA particles for delivery of vaccines to prevent M. tuberculosis infection. 相似文献