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乙型流行性感冒病毒的基因快速检测 总被引:1,自引:2,他引:1
〔目的〕建立乙型流行性感冒病毒的核酸快速诊断技术。〔方法〕采用RT -PCR方法对乙型流感病毒的HA基因进行检测 ,我们对乙型流感病毒的HA基因保守区域设计相关引物 ,进行扩增 ,并作了特异性与灵敏度比较。〔结果〕该引物只能扩增乙型流感病毒的特异性片段 ,而对其它型别的流感病毒以及麻疹、风疹、腮腺炎病毒无交叉反应。检测的灵敏度一次PCR可达 1TCID50 ,二次PCR反应可达 0 .1TCID50 。〔结论〕采用该方法从临床患者含漱液标本中的检出率 ,比采用MDCK细胞分离的阳性率更高 ,可达到迅速、正确地诊断乙型流感的实用目的。 相似文献
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本文着重讨论了病案管理如何促进医疗、教学、科研的发展。病案管理人员在为医、教、研服务的过程中,要不断更新自己的知识水平,提高自身素质。运用高新技术,采用科学的病案管理模式。变被动为主动,及时、有效、准确地为医、教、研提供最有价值的资料、数据。同时实现自身价值和病案价值。 相似文献
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食用合成色素的检测方法 总被引:1,自引:0,他引:1
我国批准使用的食用合成色素有21种,其中最常用的有胭脂红、苋菜红、柠檬黄、日落黄和亮蓝等,食用合成色素多为含有R-N=N-R'键、苯环或氧杂蒽结构化合物,它们对人体存在一定的不安全性或者产生有害作用[1]。因此,使用范围限于饮料及配制酒、糖果、糕点和青梅等,禁止用于肉类、鱼类、水果及其制品(红肠肠衣除外)等[2]。然而,市场上滥用食用合成色素的违法行为有扩展趋势,如在市售花生米、葡萄酒、肉禽制品上均可检出食用合成色素[3-5]。造成滥用食用合成色素的原因,一方面是利益驱使,另一方面是检测方法不够简便、快速,使基层单位未能很好… 相似文献
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目的探讨丝裂霉素C(MMC)预防浅表性膀胱癌等离子电切术(PKRBT)后复发的有效性。方法73例浅表性膀胱癌病人行PKBRT后即MMC 40 mg膀胱内灌注,常规MMC膀胱灌注12月。结果术后6月复发3例(4.1%),12月复发11例(15.1%),20月复发15例(20.5%)。结论MMC膀胱内灌注在PKRBT术后预防复发疗效良好。 相似文献
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In vitro studies of enantioselective metabolism of R-(+)- and S-(-)verapamil (VER) were conducted using human cDNA-expressed CYP3A isoforms, CYP3A4, CYP3A5, and CYP3A7. N-dealkylated products nor-VER [2,8-bis-(3,4-dimethoxyphenyl)-2-isopropyl-6-azaoctanitrile] and D617 [2-(3,4-dimethoxyphenyl)-5-methylamino-2-isopropylvaleronitrile] were the major metabolites for all CYP3A isoforms regardless of enantiomer. Enantioselectivity of CYP3A4 and CYP3A7 was most similar among the three isoforms. This coincides with the degree of homology of amino acids at the active sites and in the total amino acid sequences of the enzymes. Biphasic substrate inhibition was observed for the formation of nor-VER and D617, whereas simple biphasic kinetics were observed for the formation of O-demethylated products for both enantiomers with CYP3A4. The biphasic substrate inhibition was observed only for nor-VER, and simple biphasic kinetics were observed for D617 and O-demethylated products for both enantiomers with CYP3A5. However, with CYP3A7, D617 and O-demethylated products showed typical Michaelis-Menten kinetics, and only nor-VER displayed substrate (monophasic) inhibition. When metabolic rates of VER were determined in the presence of three different effectors, midazolam, testosterone, and nifedipine, activation, inhibition, or activation and inhibition of VER metabolism was observed depending on the enantiomers, metabolites, effectors, and cytochrome P450 isoforms. Addition of anti-CYP3A4 antibody inhibited formation of all metabolites for both CYP3A4 and CYP3A5. The atypical phenomena (biphasic substrate inhibition, activation, and inhibition depending on product formation) of VER kinetics could be adequately explained by introducing the concept of steric interaction into a two binding-site model. 相似文献
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Fang Zhao Puhong Zhang Lu Zhang Wenyi Niu Jianmei Gao Lixin Lu Caixia liu Xian Gao 《Nutrients》2015,7(4):2719-2730
In China, few people are aware of the amount and source of their salt intake. We conducted a survey to investigate the consumption and sources of dietary salt using the “one-week salt estimation method” by weighing cooking salt and major salt-containing food, and estimating salt intake during dining out based on established evidence. Nine hundred and three families (1981 adults and 971 children) with students in eight primary or junior high schools in urban and suburban Beijing were recruited. On average, the daily dietary salt intake of family members in Beijing was 11.0 (standard deviation: 6.2) g for children and adolescents (under 18 years old), 15.2 (9.1) g for adults (18 to 59 years old), and 10.2 (4.8) g for senior citizens (60 years old and over), respectively. Overall, 60.5% of dietary salt was consumed at home, and 39.5% consumed outside the home. Approximately 90% of the salt intake came from cooking (household cooking and cafeteria or restaurant cooking), while less than 10% came from processed food. In conclusion, the dietary salt intake in Beijing families far surpassed the recommended amounts by World Health Organization, with both household cooking and dining-out as main sources of salt consumption. More targeted interventions, especially education about major sources of salt and corresponding methods for salt reduction should be taken to reduce the risks associated with a high salt diet. 相似文献