米口袋化学成分的研究

从米口袋（Gueldenstae dtia multiflora Bge.）中分离得到11个化合物,应用光谱学和化学方法鉴定了其中7个化合物的结构,分别为黑麦草内酯、烈香杜鹃素Ⅰ、木栓醇、木栓酮、4-烯-3-酮豆甾烷、A’-Neogammacer-22（29）-en-3β-ol,acetate和β-谷甾醇。7个化合物均从该植物中首次分离得到,其中化合物Ⅰ是该植物中的水溶性成分。     

三孔法微型腹腔镜在胆囊切除术中的应用

目的　探讨三孔法微型腹腔镜在胆囊切除术中的临床应用价值。　方法　 90例慢性结石性胆囊炎和胆囊息肉样病变随机分成两组 ,分别进行三孔法微型腹腔镜下胆囊切除术 (Mini-laparoscop iccholecystectomy ,MLC)和常规孔法腹腔镜胆囊切除术 (Laparoscopiccholecystectomly,LC)。比较两组的中转率、手术时间、术后 6小时疼痛程度和并发症。　结果　两组均无并发症发生。MLC组中 2例因操作困难 ,中转LC。MLC组与LC组手术时间分别为 (5 4 8± 11 3)min和 (5 1 0± 11 0 )min ,(t =1 6 1,P >0 0 5 ) ;术后疼痛程度分别为 (2 0± 0 7)分和 (2 5± 0 8)分 ,(t=- 3 0 2 ,P <0 0 1)。　结论　在熟练掌握LC的基础上 ,开展MLC是安全、有效的。     

第三方调解机制在处理医疗纠纷中的运用

国内医疗纠纷案件的大量增加,占据了大量的司法资源,也严重影响了医患双方的利益,本文通过对第三方调解模式的探讨,希望建立医患之间的第三者,在医疗纠纷案件的处理中发挥积极作用,妥善有效地处理医疗纠纷案件、促进社会的和谐发展。     

新生儿难治性先天性乳糜胸的临床分析

目的 探讨难治性先天性乳糜胸(CC)新生儿的临床特点、治疗方法.方法 选择2015年6月至2019年12月,在首都儿科研究所附属儿童医院住院治疗的11例难治性CC新生儿为研究对象,回顾性分析其临床表现、辅助检查结果、治疗、转归和随访情况.本研究遵循的程序符合2013年新修订的《世界医学协会赫尔辛基宣言》,并与患儿监护人...     

PTEN基因表达对EC9706细胞增殖、侵袭、凋亡能力的影响

目的:观察PTEN基因表达水平的变化对食管癌EC9706细胞增殖、侵袭、凋亡能力的影响。方法:构建2个靶向PTEN的siRNA载体及1个PTEN表达载体,分别转染EC9706细胞。实验分沉默1组、沉默2组、siRNA载体对照组、PTEN表达组、表达载体对照组和未转染组。RT-PCR、Westernblot法检测转染后各组细胞PTEN基因mRNA和蛋白表达水平。采用CCK-8试验、软琼脂克隆形成实验、流式细胞术和Transwell试验分别检测各组细胞增殖、克隆形成能力、细胞凋亡率及侵袭能力。结果:与未转染组比较,沉默1组、沉默2组PTENmRNA和蛋白表达量均降低,PTEN表达组PTENmRNA相对表达量升高(F=25.762,P<0.001);与未转染组比较,PTEN表达组细胞软琼脂克隆形成数和穿透细胞数均降低,细胞凋亡率增加(F=50.752、29.981、32.193,P均<0.001)。结论:上调PTEN的表达水平可以有效抑制EC9706细胞的增殖、克隆形成和侵袭转移能力,同时促进细胞凋亡。     

香砂六君子汤治疗功能性消化不良的Meta分析

目的：评价香砂六君子汤治疗功能性消化不良临床疗效。方法：通过计算机检索中文数据库,收集香砂六君子汤治疗功能性消化不良临床研究。按纳入及排除标准筛选文献,采用Jadad质量评分表对文献进行评价,提取有效数据,用RevMan5.0软件包进行统计分析。结果：临床治疗有效率：异质性检验P〉0.1,表明研究数据具有同质性,故采用固定效应模式。合并效应量RR=1.21,95%CI（1.14,1.29）;合并效应量的检验Z=11,P〈0.00001。表明香砂六君子汤治疗功能性消化不良总效率相对西药有明显优势。漏斗图显示不对称,提示纳入评价的文献存在发表性偏倚。结论：香砂六君子汤治疗功能性消化不良疗效明显优于西药治疗。     

Interaction of Hepatitis B Virus X Protein with the Pregnane X Receptor Enhances the Synergistic Effects of Aflatoxin B1 and Hepatitis B Virus on Promoting Hepatocarcinogenesis

Background and AimsHepatitis B virus (HBV) infection has been found to increase hepatocellular sensitivity to carcinogenic xenobiotics, by unknown mechanisms, in the generation of hepatocellular carcinoma. The pregnane X receptor (PXR) is a key regulator of the body’s defense against xenobiotics, including xenobiotic carcinogens and clinical drugs. In this study, we aimed to investigate the molecular mechanisms of HBV X protein (HBx)-PXR signaling in the synergistic effects of chemical carcinogens in HBV-associated hepatocarcinogenesis.MethodsThe expression profile of PXR-cytochrome p450 3A4 (CYP3A4) signaling was determined by PCR, western blotting, and tissue microarray. Cell viability and aflatoxin B1 (AFB1) cytotoxicity were measured using the cell counting kit-8 assay. Target gene expression was evaluated using transient transfection and real time-PCR. The genotoxicity of AFB1 was assessed in newborn mice with a single dose of AFB1.ResultsHBx enhanced the hepatotoxicity of AFB1 by activating CYP3A4 and reducing glutathione S-transferase Mu 1 (GSTM1) in cell lines. Activation of PXR by pregnenolone 16α-carbonitrile increased AFB1-induced liver tumor incidence by up-regulating oncogenic KRAS to enhance interleukin (IL)-11:IL-11 receptor subunit alpha-1 (IL11RA-1)-mediated inflammation in an HBx transgenic model.ConclusionsOur finding regarding AFB1 toxicity enhancement by an HBx-PXR-CYP3A4/ GSTM1-KRAS-IL11:IL11RA signaling axis provides a rational explanation for the synergistic effects of chemical carcinogens in HBV infection-associated hepatocarcinogenesis.     

Application of collagen triple helix repeat containing‐1 and mitotic spindle apparatus antibody in small cell lung cancer diagnosis

BackgroundThe clinical significance of serum collagen triple helix repeat protein‐1 (CTHRC1) and mitotic spindle apparatus antibody (MSA) in the diagnosis of small cell lung cancer (SCLC).MethodsOf the 229 lung tumor patients selected, 62 patients were divided into SCLC, 94 patients with non‐small cell lung cancer (NSCLC), and 73 patients with benign lung disease (BLD). The health controls (HC) had a span of 66 cases with normal physical condition. The serum extracted from each participator and enzyme‐linked immunosorbent assay was adopted for measuring the serum CTHRC1 and MSA; in the meantime, automatic electrochemiluminescence immunoassay was used for the quantitative determination of serum NSA and CEA. And then, the differences in serum CTHRC1, MSA, NSE, and CEA were compared among involved groups.Results① Compared with other groups, the concentrations of CTHRC1, MSA, and NSE showed a marked increase in the group of SCLC (all p < 0.01). Especially for SCLC patients with lymph node metastasis, CTHRC1 provided a notably higher level than those without metastasis. ② CTHRC1 and MSA established a diagnostic criterion with the specificity of 90.99% and 86.27% for SCLC, respectively. ③ In series, the specificity of CTHRC1 and NSE was the highest (99.30%), while MSA and NSE had the highest sensitivity (96.72%) in parallel. ④ Both CTHRC1 and MSA were hazardous factors interconnected with SCLC.ConclusionSerum CTHRC1 and MSA had a more exciting prospect of application. When used in conjunction with NSE and CEA, they could optimize the clinical diagnosis value of SCLC.     

河南农村成年人高血压患病率及影响因素

目的:了解河南省农村地区成年人高血压的患病率及其影响因素.方法:2007年7月至2008年7月,采用随机整群抽样的方法调查河南省新安县35～74岁常住居民高血压的患病率,并采用logistic回归分析其影响因素.结果:共调查16 953人,高血压的粗患病率为33.2%.用2000年全国人口数据标化后,高血压患病率为29.8%,女性高于男性(χ2=81.979,P=0.001).高血压患病串随年龄增加而升高(χ2=1 321.850,P<0.001).在男性中,肥胖(OR=2.952,95%CI=2.443～3.567)、高血压家族史(OR=2.281,95%CI:1.994～2.610)和糖尿病(OR=1.500,95%CI:1.202～1.870)是高血压的独立危险因素,体力活动(OR=0.825,95%CI=0.763～0.891)是其独立保护因素;在女性中,肥胖(OR=2.732,95%CI=2.428～3.075)、高血压家族史(OR=2.573,95%CI:2.320～2.853)和糖尿病(OR=1.334,95%CI:1.131～1.573)是高血压的独立危险因素,体力活动(OR=0.890,95%CI:0.839～0.944)是其独立保护因素.结论:河南农村成年人具有很高的高血压患病率,应控制肥胖、糖尿病和血脂异常,从而防治高血压.     

HSVⅡ-DNA在生殖器疱疹检测中的应用

目的探讨HSVⅡ-DNA检测对生殖器疱疹中Ⅱ型单纯疱疹病毒（HSVⅡ）阳性检出率的应用价值。方法选取本院2010年7月～2012年7月性病门诊收治的生殖器疱疹患者135例为实验标本,分别予以荧光定量聚合酶链反应法（FQ-PCR）检测HSVⅡ-DNA和血清酶联免疫吸附法（ELISA）检测HSVⅡ抗体,再以病毒培养法评价以上两种方法的检出率。结果FO-PCR法HSVⅡ-DNA检测的HSVⅡ阳性检出率为74．1％,ELISA法HSVⅡ抗体检测的HSVⅡ阳性检出率为65．2％,作病毒培养标准检测的HSVⅡ阳性率为80．7％;FQ-PCR法检测HSVⅡ的灵敏度、阳性及阴性预测值、特异性显著高于ELISA法。结论FQ-PCR法HSVU-DNA检测的HSVⅡ阳性检出率更贴近于金标准值．并且检测上具有特异性强、灵敏度高、快速等优越性,对于临床诊断生殖器疱疹具有重要的应用价值,值得推广应用。