HPLC手性固定相法拆分四氢呋喃-2-甲酸甲酯对映体

目的 采用HPLC手性固定相法拆分四氢呋喃-2-甲酸甲酯对映体。方法 采用Chiralcel OD-H(250 mm×4.6 mm, 5μm)色谱柱,流动相为正己烷-异丙醇(90:10),流速1.0 mL·min^-1,柱温25℃,检测波长220 nm。结果 四氢呋喃-2-甲酸甲酯对映体的分离度大于3.3,分析时间在10 min内。结论 所用方法快速、温和、分离度高,为该对映体的合成及质量控制提供了一种简单高效的检测方法。     

西宁地区冠心病患者血浆铁蛋白血脂水平测定的意义

目的 :探讨血浆铁蛋白及血脂水平在西宁地区冠心病诊治中的价值。方法 :西宁地区经临床确诊的冠心病患者 6 5例 ,其中冠心病合并高血压 30例 ,采清晨空腹静脉血 5ml,应用日立7170s全自动生化仪测定血浆铁蛋白、血脂 (TCh、TG、HDL -c、LDL -c)。 30例健康查体者为对照组。结果 :本地区冠心病患者血浆铁蛋白水平较对照组明显降低 (P <0 .0 1) ,LDL -C水平较对照组增高 (P <0 .0 5 )。结论 :高海拔地区冠心病患者血浆铁蛋白浓度显著低于正常对照组。较低的铁蛋白水平提示存在着较高的血清游离铁水平 ,促进了LDL的脂质氧化 ,加快了动脉粥样硬化斑块的形成     

健康随访管理模式在早期诊断恶性肿瘤中的实践探讨

目的:探讨通过建立健康体检随访管理模式,对早期筛查恶性肿瘤的临床价值。方法:对2007年-2009年106033人健康体检人群中筛查出的肿瘤早期疑似病例,建立健康档案进行随访检查与跟踪。结果:通过健康管理随访,从5796例疑似人群中筛查出早期恶性肿瘤患者84人,检出率为79.22/10万,其中肺癌(19.8/10万)、肾癌(13.2/10万)、肝癌(8.5/10万)占据检出率前3位。结论:健康体检结合疑似病例健康管理随访检查与跟踪是早期发现恶性肿瘤的重要手段与有效途径。     

不典型肝脓肿的低场MRI诊断(附13例报告)

目的:探讨不典型肝脓肿在低场MRI中的表现特征。方法回顾性分析13例经病理、临床证实为不典型肝脓肿的MRI表现。结果①所有病灶均位于肝脏边缘部分;②楔形征像;③在增强扫描的动脉期、静脉期和延迟期分别依次出现楔形强化、分隔状强化和延迟强化、“花瓣”征。结论上述征象为不典型肝脓肿在低场MRI中的特征表现。T2WI脂肪抑制序列和动态增强扫描对鉴别诊断有价值。     

“A” protein of amyloidosis isolation of a cross-reacting component from serum by affinity chromatography

A nonimmunoglobulin protein (A protein) has been isolated from amyloidotic tissue of secondary type. Antisera prepared to this protein identified a cross-reacting substance in the sera of patients with secondary amyloidosis. Sera from 70 persons with amyloidosis, 120 normal adults, 20 aged persons, and 97 patients with chronic diseases were tested for this substance. One hundred percent of secondary amyloid sera had amounts of amyloid serum component detectable by double diffusion in agar, whereas only 19% of primary amyloid sera were positive. Approximately 60% of rheumatoid sera as well as 60% of sera from aged individuals were positive. Only 3% of normal blood donors had detectable amounts of this circulating substance. Isolation of the serum component by affinity chromatography and partial characterization have shown that it is an α-globulin with a molecular weight of 100,000–120,000, that it is not related antigenically to immunoglobulin or amyloid P-component, and that it has an amino acid analysis that is markedly different from tissue A protein. The possible participation of this substance in the genesis of amyloid is discussed.     

Fluoro-Jade C揭示小鼠匹罗卡品模型中杏仁核神经变性

目的应用Fluoro-Jade C(FJC)染色方法在小鼠匹罗卡品癫痫模型中探测杏仁核结构中神经元的变性情况,以了解杏仁核结构在慢性颞叶癫痫发生中的病理变化和癫痫反复发作的神经基础。方法雄性昆明小鼠6只(对照组3只,匹罗卡品处理组3只)。在癫痫持续状态后12 h处死处理组小鼠。在杏仁核水平切制冠状切片,行FJC染色,在荧光显微镜下观察FJC阳性细胞的形态和在杏仁核中的整体分布情况。结果在处理组,FJC染色的脑切片上可以很清楚地看到呈亮黄绿色荧光的FJC阳性细胞,呈神经元形态,胞体和突起均清晰显示。杏仁核出现大量FJC阳性细胞,而对照组未见。结论该研究在小鼠匹罗卡品癫痫模型中运用FJC染色技术显示杏仁核中发生了大量神经元变性,有利于更好地理解颞叶癫痫中中枢神经系统所发生的病理变化和自发反复发作的癫痫机制。     

冷极射频序贯索拉非尼与索拉非尼单药治疗进展期肝细胞癌的对比研究

目的比较冷极射频序贯索拉非尼与索拉非尼单药治疗进展期肝细胞癌的疗效和不良反应。方法将22例晚期肝细胞癌患者随机分为观察组(10例,冷极射频序贯索拉非尼治疗)和对照组(12例,索拉非尼单药治疗),治疗3个月后比较两组患者的治疗有效率、临床获益率及生存期;同时观察比较两组患者的不良反应。结果治疗后观察组的治疗有效率为60.0%,临床获益率为80.0%;对照组的治疗有效率为8.3%,临床获益率为66.7%,观察组的有效率及临床获益率较高(P<0.05);且观察组的6、12个月生存率及中位无进展生存时间均优于于对照组(P<0.05);两组患者的不良反应发生率差别无统计学意义(P>0.05)。结论与索拉非尼单药治疗相比较,冷极射频序贯索拉非尼治疗进展期肝细胞癌疗效更好,安全性相似,值得临床推广应用。     

Role of arachidonoylethanolamine in blood pressure regulation in volume-resistant patients on peritoneal dialysis

In this study we explored the possible role of arachidonoylethanolamine (AEA) in regulating blood pressure in patients on continuous ambulatory peritoneal dialysis (CAPD). One hundred and five patients on CAPD were enrolled. Volume status was evaluated by the overhydration (OH) value obtained by bioimpedance analysis. OH?<?2.0?kg was defined as normal volume (NV) and OH????2.0?kg as high volume (HV). Home mean systolic blood pressure <130?mmHg was defined as controlled hypertension (CHT) and ??130?mmHg as uncontrolled hypertension (UHT). The patients were divided into four subgroups: (1) controlled hypertension with normal volume (CHT-NV), (2) controlled hypertension with high volume (CHT-HV), (3) uncontrolled hypertension with normal volume (UHT-NV), and (4) uncontrolled hypertension with high volume (UHT-HV). AEA was measured by ultra performance liquid chromatography-tandem mass spectrometry. AEA was significantly higher in the HV group as compared with the NV group (P?<?0.05). In addition, AEA was also significantly higher in the CHT-HV group as compared with the UHT-NV group (P?<?0.05). These results may suggest a compensatory function of AEA and TRPV1 pathway to lower blood pressure during volume expansion in CAPD patients.