江西省528例非何杰金氏恶性淋巴瘤回顾性病理分析

本文收集了我省十年(1974—1983)来诊断为恶性淋巴瘤的病例,按免疫功能分类复查了全部切片,最后确诊为非何杰金氏恶性淋巴瘤(NHL)528例,进行了分析。本组NHL在首发部位、类型分布等方面与国内外有所不同。本组NHL首发于淋巴结外的占64.02％,明显高于国内其它省、市,而滤泡型淋巴瘤则低于国内多数地区。T细胞淋巴瘤占14.84％,较国内、外均低。并提出提高制片质量和广泛开展及应用免疫学技术的重要性。     

Preparation of [125I-Tyr27,Leu5]beta h-endorphin and its use for crosslinking of opioid binding sites in human striatum and NG108-15 neuroblastoma-glioma cells.

A radioligand suitable for crosslinking studies to opioid receptors has been obtained by radioiodination and purification of the monoiodotyrosine-27 derivative of the synthetic human beta-endorphin (beta h-endorphin) analogue [5-leucine]beta h-endorphin. The derivative, [27-[125I]monoiodotyrosine,5-leucine]beta h-endorphin, was crosslinked to human striatal (caudate and putamen) and NG108-15 neuroblastoma-glioma cell membranes by using disuccinimidyl suberate. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis under reducing conditions revealed four specifically labeled bands at 68, 40, 30, and 25 kDa for both human caudate and putamen, whereas NG108-15 cell membranes gave specifically labeled bands at 92, 56, 38, and 23 kDa.     

原发性甲状腺机能亢进症的手术探查技巧

目的探讨原发性甲状腺机能亢进症的手术探查技巧和经验教训。方法对50例原发性甲状腺机能亢进症患者临床资料进行回顾性总结分析。结果48例患者手术治疗，术后38例诊断为甲状旁腺瘤，6例为甲状旁腺增生，4例为甲状旁腺癌。48例中44例第一次手术探查成功（占88％），2例行2次手术，2例行3次手术。46例术后痊愈，2例并发多处肋骨骨折发生呼吸衰竭而死亡。结论确诊为原发性甲状腺机能亢进症伴有临床征象者均应手术治疗。术前病灶定位明确者手术较容易，定位不明确者应在“热区内”重点探查。     

对比因素对儿童分辨物体细节能力的影响

目的观察对比因素对儿童分辨物体细节能力的影响,了解儿童对比视力的状况。方法使用对比视力表,测量53名裸眼视力≥5.0的正常儿童92%,52%,30%对比水平处的对比视力。结果儿童的对比视力随对比度的降低而显著下降,对比度与视力之间存在显著相关;左、右眼的对比视力差异无统计学意义;男性和女性的对比视力差异无统计学意义。结论低对比的学习环境会增加儿童视觉负荷,在防治学生近视过程中应引起重视。     

核黄素等抗甲基苄基亚硝胺的致细胞突变作用

采用ＣＨＯ细胞ＨＧＰＲＴ位点突变测定系统观察了核黄素、尼克酰胺和锌等微量营养素对甲基苄基亚硝胺（ＭＢＮＡ）致细胞突变作用的影响。结果表明，在ＲＰＭＩ１６４０培养基中添加核黄素（２．６～１３．０μｍｏｌ／Ｌ）或尼克酰胺（４０～１２０μｍｏｌ／Ｌ）可显著抑制ＭＢＮＡ的致细胞突变作用。联合应用核黄素、尼克酰胺和锌效果更佳。提示：核黄素等微量营养素可能具有阻止细胞癌变的作用，为应用微量营养素干预治疗癌变过程提供了一定的理论依据。     

等容血液稀释治疗高血压病75例疗效观察

本文报告等容血液稀释（简称ＤＨ）治疗高血压病７５例，总有效率为９０．２％，４８例随访２年血压下降稳定持久，血液流变学改善，总有效率为８７．５％。结果表明：ＨＤ治疗高血压病可以改善血流高粘滞状态，降低血液粘度，增强血液流动性，减少血流阻力，改善微循环灌流，从而达到降低血压和改善器官血流，达到持久降压及减少器官合并症。一次ＨＤ与降压药适量配伍，疗效更显著而持久，可维持数月至２年以上。     

Comparison of iodine-123 low-density lipoprotein (LDL) and indium-111 LDL binding to mononuclear cells of healthy normolipaemic controls and patients with heterozygous familial hypercholesterolaemia

The binding of radiolabelled lipoproteins, iodine-123-labelled low-density. lipoprotein (LDL) and indium-111-labelled LDL, to peripheral blood mononuclear cells (MNCs) was compared in normolipaemic subjects and in patients with heterozygous familial hypercholesterolaemia (FH). ¹²³I-LDL and ¹¹¹In-LDL binding to MNCs exhibited high-affinity, highly specific, time- and temperature-dependent binding reaching saturation at concentrations above 50 nM. The number of LDL binding sites (B_max) was significantly (P<0.01) lower in FH patients (P<0.001; ¹²³I-LDL: B_max 279±44 ng protein/10⁸MNCs; ¹¹¹In-LDL: B_max 309±43 ng protein/10⁸MNCs) as compared with controls (¹²³I-LDL: B_max 2874±246 ng protein/10⁸ MNCs; ¹¹¹In-LDL: B_max 3145±339 ng protein/10⁸ MNCs). The corresponding dissociation constants (K _d) were 16±8 nM for ¹²³I-LDL and 12±6 nM for ¹²³In-LDL in healthy volunteers (¹²³In-LDL vs ¹¹¹In-LDL, P<0.05). In FH patients, the K _d values were 20±8 nM for ¹²³I-LDL and 16±6 nM for ¹²³In-LDL (P<0.05 vs controls for both ¹²³I-LDL and ¹¹¹In-LDL). ¹¹¹In-LDL binding to MNCs was inhibited (IC₅₀) by 30±8 nM in healthy controls and 38±12 nM in FH patients (P<0.05). ¹²³In-LDL binding to MNCs was inhibited (IC₅₀) by 34±8 nM in healthy controls and 46±10 nM in FH patients (P<0.05). Taken together, these results suggest a reduced number of LDL receptors expressed on MNCs from FH patients. We conclude that ¹¹¹In-LDL and ¹²³I-LDL are equally well suited as a probe of receptor-mediated binding and uptake of LDL.     

心脏机械瓣膜置换术后分娩的临床研究

目的 探讨心脏机械瓣膜置换术后分娩的临床处理措施。方法 对19例心脏机械瓣膜置换手术后妊娠及分娩的资料进行回顾性分忻。结果 19例心脏机械瓣膜置换术后产妇均安全度过孕产期。分娩前后心功能Ⅰ～Ⅲ级，分娩时出血200～260m1，胎儿体重2950--3146g。无母婴死亡者，无胎儿出血以及畸形者。结论 心脏机械瓣膜置换术后心功能Ⅰ～Ⅲ级可安全度过孕产期。分娩采用剖宫产术为宜。妊娠期间口服抗凝剂(华法林)不增加分娩时母婴出血和新生儿畸形。     

160例女性不孕症的四种病原体检测及意义

本文报告了对１６０例女性不孕症患者宫颈分泌物中解脲脲原体（Ｕｕ）、人型支原体（Ｍｈ）、沙眼衣原体（Ｃｔ）和淋病奈氏球菌（Ｎｇ）的测定，结果总体检出率７０％（１２０／１６０），明显高于对照组（同期早孕妇女），Ｐ＜０．０１。其中输卵管阻塞组阳性率８４．７８％（３９／４６），明显高于输卵管通畅组６３．４６％（３３／５２）及欠通畅组６４．５２％（４０／６２），Ｐ＜０．０５。且针对性治疗收到一定效果，提示妇女下生殖道Ｕｕ、Ｃｔ、Ｎｇ感染是输卵管阻塞性不孕的主要原因之一。对该类患者行三项检测，对不孕症的病因诊断及治疗有好处。