检测胞浆抗原用于急性白血病免疫分型

目的:探讨胞浆抗原在急性白血病免疫分型中的临床意义。方法:用流式细胞仪检测42例初治急性白血病患者胞浆抗原CyCD_3,CyCD_(22),CyCD_(79a)和MPO。结果:T—ALL阳性胞浆抗原主要表达在CyCD_3,阳性率占88.9％。B—ALL阳性胞浆抗原主要表达在CyCD_(79a),AML主要表达CyMPO。结论:CyCD_3和CyCD_(79a),及MPO可分别作为T—ALL,B—ALL和AML系列特异性标志,对正确诊断白血病提供非常有价值的依据。     

PCNA在乳腺病变组织中的表达与病理学特性关系

本文运用增殖细胞核抗原(PCNA)单克隆抗体对88例乳腺病变组织进行免疫组化研究,并与乳腺癌组织学分级和淋巴结转移作了对比分析,结果显示:乳腺良性病变阳性率为16%,乳腺癌为73.02%,两者比较有显著性差异P<0.01),pCNA指数与乳腺癌组织学分级淋巴结转移密切相关.作者认为PCNA的表达对区分良恶性病变及组织学分级,淋巴(?)转移(?)为客观可靠,是(?)癌预后判断的良好指标.     

抑制剂对酪氨酸酶影响的初步研究

采用酶动力学及聚丙烯酰胺凝胶电泳方法研究了ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ、ＤＤＣ对酪氨酸酶的抑制作用。ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ为非竞争性抑制作用，随着抑制剂浓度增加，酶活性逐渐减弱。当ＮａＨＳＯ３、ＮａＮ３、ＫＳＣＮ的浓度分别达到１．２ｍｍｏｌ／Ｌ１２。５ｍｍｏｌ／Ｌ１２．５ｍｍｏｌ／Ｌ时，酶活性的抑制率为１００％、５０％、２４％。ＤＤＣ对酪氨酸酶的抑制作用为竞争性抑制作用，其浓度达４ｍｍｏｌ／Ｌ时，酶活性１００％受到抑制。     

Comparison of two-dimensional vs three-dimensional camera systems in laparoscopic surgery

Background: The lack of depth perception and spatial orientation in video vision are the drawbacks of laparoscopic surgery. The advent of a three-dimensional camera system enables surgeons to regain binocular vision and may be advantageous in complex laparoscopic procedures. Methods: We prospectively studied two groups of surgeons (with and without experiences in laparoscopic surgery) who performed a designated standardized laparoscopic task using a two-dimensional camera system (Olympus OTV-S4) vs a three-dimensional camera system (Baxter-V. Mueller VS7700) and compared their time performances. Results: The results suggested that only experience in laparoscopic surgery had significant effect on individual's performance. We could not demonstrate any superiority of the 3D system over the 2D system. However, two-thirds of the surgeons commented that the depth perception did improve. Conclusions: With further refinement of the technology, the 3D system may improve its potential in laparoscopic surgery.     

Injuries to bicyclists in Wuhan, People's Republic of China.

OBJECTIVES: This study examined the morbidity and mortality from bicycling injuries in Wuhan, China. METHODS: Police department data for the year 1993 complemented by data from emergency room interviews were analyzed. RESULTS: The death rate from bicycling injuries was estimated as 2.2 per 100000 population, more than seven times the rate for the United States. At least 79% of the fatalities and 17% of the emergency room cases sustained head injuries, the majority (71%) of which resulted from contact of the head with the concrete or asphalt road. None of the patients was wearing a helmet at the time of injury, and helmet use among the general bicyclist population was nonexistent. CONCLUSIONS: Bicycle-related head injury is an important public health issue in China. The effectiveness of safety helmets in developing countries needs to be evaluated.     

PCR检测角膜内单疱病毒DNA的实验及临床研究

为了探索单疱病毒性角膜炎的发病机制和快速诊断ＨＳＫ。方法应用多聚酶链反应对感染的ＨＳＫ的２０只纯新西兰白兔和６０例ＨＳＫ患者的角膜进行了单疱病毒－１－ＤＮＡ检测。结果急性感染期的５只兔角膜和１２只人角膜均阳性；用稳定期４５ｄ的兔角供体行部分穿透性角膜移植术，术后５０ｄ１０只兔角膜中７只阳性，稳定期６个月－６年的１８只人角膜片，１２只阳性；３０例临床可疑ＨＳＫ角膜刮取物，２６例阳性；５只未感染ＨＳＫ     

低剂量混配农药对家兔血浆谷胱甘肽过氧化物酶和一氧化氮的影响

目的 探讨低剂量混配农药对家兔脂质过氧化及一氧化氮（NO）浓度的影响及意义。方法 将家兔随机分为6个混配农药染毒组、1个丙溴磷染毒组和1个对照组，于不同的时间测定各组血清胆碱酯酶（ChE)活力、谷胱甘肽过氧化物酶（GSH-Px)活力及一氧化氮（NO）浓度。结果 除较高剂量混配农药组外，其余各组染毒后的ChE活力均大于对照组实验前平均值的70%。随着染毒时间的延长农药混配组血浆GSH-Px活力高于或显著高于同时间的单剂量组和对照组，而血浆NO的浓度则呈降低趋势。结论 低剂量含有机磷的混配农药在导致ChE活力降低之前即可造成脂质过氧化增强和NO浓度的降低。     

通脉灵对心肌缺血再灌注犬心功能的保护作用

本实验复制２４只杂种犬心肌缺血再灌注损伤病理模型，应用与生理盐水、丹参对比的方法观察通脉灵对血液动力学及心功能的影响。结果表明，通脉灵小剂量组（ＴＭ１Ｇ）、通脉灵大剂量组（ＴＭ２Ｇ）和丹参对照组（ＤＳＧ）的心输出量（ＣＯ），心脏指数（ＣＩ），心搏指数（ＳＩ）、左室内压峰值（ＬＶＳＰ）及左室内压最大变化速率（±ｄｐ／ｄｔｍｓｘ）均明显高于生理盐水对照组（ＮＳＧ）。本实验证明，通脉灵主要是通过保护心肌舒缩功能改善血液动力学指标进而防止缺血再灌注时心功能不全的发生。     

P53基因突变与宫颈癌关系的研究

目的：通过对宫颈癌Ｐ５３基因７－８外显子突变的研究，探讨该基因的突变与宫颈癌的发生、发展之间的关系。方法：本文运用聚合酶链式反应（ＰＣＲ）方法，对宫颈癌组织Ｐ５３的７－８外显子是突变进行了试验，并与非肿瘤的慢性宫颈炎（宫颈糜烂、宫颈息肉）组织作了对照。结果：１０例宫颈癌标本中有７例出现了Ｐ５３７－８外显子的突变，阳性率高；而８例作为对照的宫颈炎标本仅有１例出现可疑的阳性结果。结果：试验结果说明，宫     

二维彩色多普勒超声心动图诊断扩张型心肌病（附100例分析）

应用ＡＴＬ－ｕｌｔｒａｍａｒｋ超６型彩色多普勒超声诊断仪诊断扩张型心肌病１００例，并与性别、年龄相应的正常人对比分析。结果：除扩张型心肌病各组室间隔及左室后壁舒张期厚度与相应正常对照组相比无显著性差异（Ｐ＞０．０５）外，其左室舒张内径及收缩内径、左房内径明显大于正常对照组（Ｐ＜０．０１），左室射血分数及左室内径缩短分数较正常对照组明显降低（Ｐ＜０．０１），并见心腔内血流速度缓慢，颜色变淡。另在７７例病人中探及４０例瓣膜返流。提示该诊断方法对扩张型心肌病具有直观、简便、准确、迅速的优点，为本病的诊断及鉴别诊断提供了良好的手段。