端粒酶反义DNA影响肺癌细胞体外生长的初步研究

目的 研究端粒酶反义核苷酸对肺癌细胞体外生长的影响。探讨针对端粒酶进行肺癌基因治疗的可行性。方法 人工合成硫化修饰六核苷酸端粒酶ＤＮＡ序列「５′－ｄ（ＴＴＡＧＧＧ）－３′」,在体外与端粒酶阳性肺癌细胞系８０１－Ｄ共同培养,观察其对肺癌细胞端粒酶活性、细胞形态和生长特性的影响。结果 端粒酶反义ＤＮＡ对端粒酶阳性肺癌细胞系８０１－Ｄ的生长和集落形成有明显的抑制作用。细胞形态发生明显改变,端粒酶反义ＤＮ     

低能超声对人肺癌细胞的体外杀伤作用

目的 探讨低能超声对人肺癌细胞的影响。方法 用低能量连续波超声处理小细胞肺癌细胞系ＳＭ、腺癌细胞系Ａ２及人肺纤维母细胞ＦＢ,用胎盘蓝染料排斥试验及克隆存活试验判定效果,结果 低能量（０．８Ｗ／ｃｍ＾２）超声３分钟分各种人肺癌细胞及人肺纤维母细胞有杀伤作用（Ｐ〈０．０５）,１０分钟可杀死全部细胞（Ｐ〈０．００１）,细胞浓度越高,杀伤作用越低,超声１０分钟后含细胞悬液的试管内温度未见显著上升,声强０．８Ｗ／ｃｍ＾２的超声使阿霉对肺癌细胞ＳＭ的杀伤作用增加１００倍（Ｐ〈０．００１）,结论 低能连续波超声可杀伤肺癌细胞,并能显著增加阿霉素对肺癌细胞的细胞毒性。     

高频超声诊断流行性腮腺炎的临床研究

目的 探讨流行性腮腺炎的声像图及彩色多普勒血流表现特征。方法 应用高频超声对临床诊断的32例流行性腮腺炎患者的腺体进行探进，观察其形态、大小、回声及周边淋巴结、组织的改变情况，并与30例同期正常同龄人进行对照。结果 受侵腺体均有不同程度的弥漫性肿大，内部回声呈非均匀性减低，可见低回声结节，腺体实质内血流显示丰富，呈“火海征”样改变；对照组未见上述声像图及血流改变；同时对单纯颈淋巴结肿大及仅颌下腺肿大者，提出了诊断和鉴别要点。结论 流行性腮腺炎的声像图表现具有特征性，高频超声对本病的诊断、鉴别诊断和病情的动态观察均具有重要的临床价值。     

大肠癌组织中CD44v6和PCNA表达及其临床生物学意义

目的：探讨CD44v6和细胞核增生抗原(PCNA)在结直肠癌组织中的表达情况与临床病理关系。方法：采用ABC和SP免疫组化方法检测84例结直肠癌组织中的CD44v6和PCNA的表达情况，用统计学x^2检验分析CD44v6和PCNA的表达意义。结果：CD44v6和PCNA在大肠癌组织中的表达具有一致性，与癌肿的浸润深度及淋巴转移、Dukes‘‘‘‘分期呈正相关，与年龄、性别及组织学类型无关。结论：结直肠癌组织中CD44v6和PCNA呈高表达是预后差的生物学指标，而高增生状态的癌细胞对化疗敏感，故对CD44v6和PCNA呈高表达患者予严格的术后化疗，可望提高疗效。     

L形颧骨截骨降低联合颞颊部除皱术临床分析

目的：探索同时解决颧骨过高及手术引起的医源性面部皮肤松垂的方法。方法：回顾分析48例颧骨过高患者的治疗过程，总结L形颧骨截骨降低联合颞颊部除皱术的设计方法和治疗效果。结果：L形颧骨截骨降低联合颞颊部除皱术较好地同时解决了颧骨过高及面中下部皮肤松弛的问题，47例患者对术后效果较满意，1例患者有轻微的两侧骨骼不对称，经Ⅱ期手术后也达到了治疗目的。结论：L形颧骨截骨降低联合颞颊部除皱术是一种较好的面部整形方法。     

Testing the chemosensitivity of renal carcinoma in vitro

Tumour cells from 23 renal carcinomas were exposed in vitro to increasing concentrations of medroxyprogesterone acetate (MPA) or one of several cytotoxic agents. Thereafter the uptake of 75Selenomethionine (75SeM) was compared to that of tumour cells not exposed to the drug. Using drug concentrations similar to those seen following in vivo administration in patients, MPA produced greater than or equal to 50% inhibition of 75SeM uptake in 5 of 23 cases. The comparable proportion for doxorubicin was 4 of 5 and for mitozantrone 7 of 12. Methotrexate and Vinblastine were much less effective. In all cases where greater than or equal to 50% inhibition was obtained with doxorubicin and mitozantrone the carcinoma had spread beyond the kidney. Thus drug sensitivity may be associated with a high mitotic rate.     

Enhancement of acetylcholine release during REM sleep in the caudomedial medulla as measured by in vivo microdialysis

Previous studies in our laboratory have found that muscle atonia could be triggered by two distinct areas of the medial medulla, a caudal region, corresponding to the nucleus paramedianus (NPM) and a rostral region, corresponding to the nucleus magnocellularis (NMC). The former region is responsive to acetylcholine (ACh) and the latter region is responsive to glutamate. In this study we have measured the endogenous ACh release across the sleep-wake cycle in these two areas with the microdialysis technique in unanesthetized, freely moving cats. We found that ACh release in NPM was state-dependent and was about 30% higher ( P0.001) during rapid eye movement (REM) sleep than during slow-wave sleep and wakefulness. However, ACh release in NMC was not selectively elevated in REM sleep. The enhancement of ACh release in NPM during REM sleep supports our hypothesis that ACh release onto cholinoceptive neurons in this area mediates the muscle atonia of REM sleep.     

Microskin autograft with pigskin xenograft overlay: a preliminary report of studies on patients.

Split-thickness pigskin graft (STPSG) was used to replace allograft skin for microskin grafting in 16 patients, nine of whom were burn patients, five suffered from traumatic defects and two from diabetic ulcers. The expansion ratios used in these patients ranged from 8:1 to 12:1. The STPSG preparation described was found to be safe for clinical application. The autogenous donor skin was excised from the inguinal area, and the donor site was primarily closed. There were no instances of donor site morbidity. The majority of the STPSG overlays adhered to the wound firmly. Histological examination showed that the microskin grafts proliferated actively immediately beneath the STPSG overlay. The time for the wound to be fully resurfaced varied from 13 to 21 days depending on the expansion ratio employed. There were only two episodes of pseudomonas infection and no further grafting was required in any of the patients. In this study the pigskin xenograft was found to provide a suitable environment for the epithelialization of microskin autografts. When allograft is not available, this is an alternative way of ensuring successful microskin grafting.     

带血运的骨移植治疗骨不连骨缺损,骨坏死77例

应用带血运的骨移植治疗骨不连、骨缺损、骨坏死骨病７７例，收到了满意的疗效。介绍了带骨膜蒂骨栓移位治疗腕舟状骨不愈合及骨缺血坏死，腰背筋膜骶棘肌髂骨瓣移位治疗腰椎不稳定，自构带血运的松质骨移植治疗长管骨、良性骨肿瘤所致的骨缺损和骨不连等手术方法。带血运的骨移植确实是治疗以上三种骨病的有效方法，并可加速治愈上述骨病减少卧床制动时间，后减少合并症的出现和功能恢复有特殊的意义。     

光照疗法所致新生儿溶血的机理及其预防

观察了接受短时间（≤４８小时）光照疗法（光疗）治疗的高胆红素血症患儿红细胞谷胱甘肽还原酶（ＧＲ）活性的改变，光疗可使红细胞谷胱甘肽还原酶活性下降，使高胆红素血症患儿溶血加剧。红细胞谷胱甘肽还原酶活性下降是由于维生素Ｂ２的光降解，致体内维生素Ｂ２不足所致。补充维生素Ｂ２可以纠正红细胞谷胱甘肽还原酶活性，从而防止光疗造成的溶血副作用。因此高胆红素血症新生儿在光疗期间常规补充维生素Ｂ２是必要的。