人卵巢组织中钙结合蛋白Calbindin-d28k蛋白和基因的表达

目的:分析钙结合蛋白Calbindin-d28k(CaBP-d28k)在人卵巢组织中的表达。方法:收集人卵巢组织标本23例,采用免疫组织化学方法和逆转录聚合酶链反应法检测CaBP-d28k在人卵巢中的蛋白及mRNA表达。结果:人卵巢中存在CaBP-d28k蛋白及其mRNA表达,CaBP-d28k蛋白定位于卵泡中的卵母细胞、颗粒细胞,以及少量的卵泡膜细胞和黄体中的粒黄体细胞和膜黄体细胞的胞浆中。卵泡各时期CaBP-d28k蛋白阳性表达强度由弱渐强依次为原始卵泡、初级卵泡、次级卵泡。结论:人卵巢中多种细胞表达钙结合蛋白CaBP-d28k,其表达随卵母细胞成熟而增强。     

Vascular endothelial growth factor expressing mesenchymal stem cells improves cardiac function in chronic myocardial infarction in pigs

Transplantation of mesenchymal stem cells （MSCs） for myocardial reconstruction has shown promise in both animal models and human phase 1 clinical studies. Vascular endothelial growth factor （VEGF） is a strong therapeutic agent for treating ischaemia by inducing angiogenesis. The feasibility of ex vivo MSCs mediated gene transfer is documented. Matsumoto and colleagues have recently reported genetically engineered MSCs carrying VEGF165 delivery for revascularization in a model of acute myocardial infarction （MI）. The promising data from our laboratory in both angiogenesis and MSCs transplantation in cunicular heart model of acute MI have prompted us to attempt the combined and simultaneous application of the two strategies.     

用于存储传输和分析的12导联同步心电图标准化系统的设计

不同厂商生产的心电图机输出的数据格式不同,这给数据的传输、交换和分析造成了很大的障碍。作者旨在建立基于web的用于心电图数据存储、传输和分析的各医院门诊之间统一的心电图标准化系统。把心电图机输出的文件(标准心电图协议格式SCP)中记录头文件采集到设计的标准数据库,实现了对不同品牌心电图机心电图存储和分析功能。     

血清碱性蛋白联合紫色反应对肿瘤的筛检价值

目的:用比色及电泳法探索肿瘤生化标志物对常见肿瘤的筛检价值。方法:用改良的血清碱性蛋白电泳(SBPE)及紫色反应检测对照组、炎症组及肿瘤组的血清碱性蛋白(SBP)及肿瘤指数。结果:两项指标对肿瘤组的阳性率为86.7%,特异性77.2%,总有效率83.5%;对常见肿瘤的阳性率分别为:子宫及卵巢癌96.6%,子宫及卵巢肿瘤95.1%,胰腺癌91.2%,肺癌91.2%。肝癌91.1%,直/结肠癌79.4%,胃癌62.5%;子宫及卵巢癌的SBP阳性率90.0%、肿瘤指数83.3%,良性肿瘤时分别为90.2%及31.7%。结论:SBP与肿瘤指数串联是常见肿瘤简单、经济、快速而灵敏的筛查方法,并在子宫及卵巢肿瘤的鉴别诊断中有意义。     

中医药治疗肩关节周围炎100例观察

2001年至2004年初,笔者采用祛风除湿,活血化瘀法治疗肩关节周围炎100例,并进行临床观察.用药后肩关节疼痛、活动受限均得到控制,肩周围恶寒、阴天下雨疼痛加重得到明显改善,总有效率93%,取得满意效果.     

GPDA 、CA72-4 、CA50 联检在胃癌诊断中的价值

 目的 探讨GPDA、CA72-4、CA50三项联检对胃癌的临床诊断价值。方法 采用电化学发光免疫分析、放射免疫法及全自动生化分析仪等技术，对125例胃癌、77例胃良性疾病、50例正常健康人血清进行GPDA、CA72-4、CAS0三项检测。结果 胃癌组GPDA、CA72-4、CA50敏感性分别为62．4％、68．0％，54．4％；特异性分别为97．6％、97．6％、98．4％。三项联检敏感性提高到89．6％，与单检比较有统计学意义（P〈0．05）；同时特异性并未明显降低，仍有93．7％。结论 GPDA、CA72-4、CA50可作为临床诊断胃癌的肿瘤标志物，三项联检可明显提高阳性检出率。     

2015—2019年江西省缺血性脑卒中患者特征及住院费用分析

目的 了解江西省2015—2019年缺血性脑卒中住院患者特征及住院费用情况，为缺血性脑卒中防控提供数据支持。方法 收集来自江西省卫生健康委员会DRGs管理系统中2015—2019年全省228家医院（69家三级综合医院，159家二级综合医院），总计381 416例出院主诊断疾病编码为I63（采用国际疾病分类第十版ICD - 10）的缺血性脑卒中患者住院信息。对住院患者特征及费用进行描述性分析。结果 2015—2019年江西省缺血性脑卒中患者,男性占比大于女性，近5年住院率由119.09/10万升高至206.61/10万。缺血性脑卒中患者最常见的共病及并发症为高血压、糖尿病、动脉粥样硬化。缺血性脑卒中次均住院费用为7 067.2(6 846.1)元，5年间，次均住院费用由7 384.0（7 353.6）元降低至6 816.9（6 769.1）元，差异具有统计学意义（H = 12.155，P＜0.002），总费用中药费占比最高，但未见明显变化趋势（H = 7.425，P = 0.063）。结论 2015—2019年江西省缺血性脑卒中住院患者住院率增长较快，高血压、糖尿病、动脉粥样硬化为3个最常见的共病,次均住院费用呈下降趋势，但住院费用负担仍较重。     

Evaluation of Adiponectin, Resistin, IL-6, TNF-a in Obese and Non-obese Women with Polycystic Ovary Syndrome

Objective To evaluate possible clinical effects of adiponectin, resistin, IL-6, and TNF-α in obese and non-obese patients with polycystic ovary syndrome (PCOS). Methods Seventy selected PCOS patients were recruited for this study, and were divided into two groups based on their body mass index (BMI): 35 obese (BMI ≥ 25kg/m2 , group A), 35 non-obese (BMI<25kg/m2 , group B). In addition, 35 healthy non-obese women (BMI<25kg/m2 ) were enrolled as the control (group C). Serum levels of FSH, LH, T, glucose, insulin, adiponectin, resistin, IL-6, TNF-α were detected, and compared the differences of them among three groups. Results Blood glucose levels among three groups had no difference (P>0.05). Blood insulin level was significantly greater in group A than in group B or group C (P<0.05), and a significant difference existed between group B and group C (P<0.05). The ratio of glucose/insulin was significantly higher in group A than in group B or group C (P<0.05), and the ratio in group B was significantly higher than that in group C (P<0.05). Adiponectin level was significantly lower and resistin level was significantly higher in group A than in group B or group C (P<0.05). IL-6 level in group C was significantly lower than that in group A or group B (P<0.05), and significant difference was found between group A and group B (P<0.05). TNF-α level was a slight high in group B, whereas there was no statistical difference among three groups (P>0.05). Conclusion Disturbances of some metabolic and inflammatory adipokines could involve the pathogenesis of PCOS in both obese and non-obese women. Low-grade chronic inflammation might have negative effects on the development of PCOS in non-obese women.     

早期慢性肾脏病患者肾组织甲状旁腺激素的表达及其作用

目的 探讨早期慢性肾脏病(CKD)患者肾组织甲状旁腺激素(PTH)表达和分布,以及其在CKD进展中的可能作用.方法 选取2009年至2012年间本科收治并经肾活检确诊的CKD 1期及2期的肾小球肾炎患者82例为研究对象.另取8例肾移植配型不符或肾肿瘤患者的正常肾组织作对照.受试者均检测Scr、BUN、血钙、磷、PTH及25(OH) VitD3等.以Cockcroft-Gault (CG)公式计算肌酐清除率(Ccr),双血浆99mTc-DTPA清除率法检测GFR.根据肾间质炎性细胞浸润程度,将患者分为轻、中、重组,用免疫组化方法观察肾组织PTH表达和分布；用Image-Pro Plus图像分析软件计算各例肾组织PTH阳性染色吸光度(A)值,并比较PTH表达强度差异.结果CKD1期及2期的肾小球肾炎患者的外周血钙、磷、25(OH) VitD3及PTH水平均处于正常范围,PTH与上述其他指标间无相关.不同病理类型肾小球肾炎患者肾组织均可见PTH表达,主要位于肾小管,而肾小球及肾间质也有少量分布,其表达强度均显著高于对照组(P＜0.01),且随肾间质炎性细胞浸润增多,PTH表达增强.各病理类型间肾组织PTH表达强度差异无统计学意义.结论 早期CKD(1期及2期)患者肾组织PTH表达增强,且早于外周血PTH的改变及矿物质和骨代谢紊乱,可能与局部炎性反应程度相关.     

Evaluation on Antisperm Antibody in Infertile Women with Chronic Salpingitis

Objective To evaluate the level of antisperm antibody （ASA） in infertile women with chronic salpingtis. Methods Forty-eight infertile women with chronic salpingtis were enrolled into this study. Serum samples were screened by the direct immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. About 50% or more of the motile sperm attaching to one or more immunobeads were regarded as clinical positivity according to the WHO criteria. About 20% 50% motile sperm that had adherent particles were deemed to be sub-positive. Results Of the 48 patients with chronic salpingtis assessed for ASA-IgG, 4 had immunobead bindings ranged from 50% to 60%, and the positive rate was 8.3%. In addition, 5 cases had 20%-40% of immunobead bindings, and the sub-positive rate was 10.4%. For ASA-IgA detecting, 2 cases with ASA-IgG positivity also had ASA-IgA immunobead bindings, and the positive rates were 22%, and 28%, respectively. Conclusion The incidence of ASA could be found in infertile women with chronic salpingtis, which suggests that an increased risk for the production of ASA would exist in the inflammatory status o f fallopian tube.