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排序方式: 共有597条查询结果,搜索用时 15 毫秒
61.
Lee BH Shin TJ Hwang SH Choi SH Kang J Kim HJ Park CW Lee SH Nah SY 《The Korean journal of physiology & pharmacology》2011,15(4):195-201
The flavonoid quercetin is a low molecular weight compound generally found in apple, gingko, tomato, onion and other red-colored fruits and vegetables. Like other flavonoids, quercetin has diverse pharmacological actions. However, relatively little is known about the influence of quercetin effects in the regulation of ligand-gated ion channels. Previously, we reported that quercetin regulates subsets of nicotinic acetylcholine receptors such as α3β4, α7 and α9α10. Presently, we investigated the effects of quercetin on muscle-type of nicotinic acetylcholine receptor channel activity expressed in Xenopus oocytes after injection of cRNA encoding human fetal or adult muscle-type of nicotinic acetylcholine receptor subunits. Acetylcholine treatment elicited an inward peak current (I(ACh)) in oocytes expressing both muscle-type of nicotinic acetylcholine receptors and co-treatment of quercetin with acetylcholine inhibited I(ACh). Pre-treatment of quercetin further inhibited I(ACh) in oocytes expressing adult and fetal muscle-type nicotinic acetylcholine receptors. The inhibition of I(ACh) by quercetin was reversible and concentration-dependent. The IC(50) of quercetin was 18.9±1.2 μM in oocytes expressing adult muscle-type nicotinic acetylcholine receptor. The inhibition of I(ACh) by quercetin was voltage-independent and non-competitive. These results indicate that quercetin might regulate human muscle-type nicotinic acetylcholine receptor channel activity and that quercetin-mediated regulation of muscle-type nicotinic acetylcholine receptor might be coupled to regulation of neuromuscular junction activity. 相似文献
62.
Kim H Park JH Shin SJ Kim MJ Bang SJ Park NH Nah YW Nam CW Joo KR Min YJ 《Chemotherapy》2008,54(1):54-62
The standard beneficial chemotherapy proven for patients with advanced pancreatic cancer is a regimen containing gemcitabine. In the pregemcitabine era, 5-fluorouracil (5-FU) was the standard agent. Oral 5-FU can be added to gemcitabine to improve the efficacy of chemotherapy and to provide better patient convenience. The possibility to improve efficacy of gemcitabine by fixed dose rate infusion (FDRI) was proposed in addition to combining it with 5-FU. We tried a new chemotherapy combining FDRI of gemcitabine with doxifluridine and leucovorin. Eligibility criteria were pathologically proven, chemotherapy-na?ve, and metastatic or nonoperable advanced pancreatic cancer. Gemcitabine 1,000 mg/m(2) was infused over 100 min (days 1, 8 and 15). Doxifluridine 200 mg/m(2) t.i.d. and leucovorin 15 mg b.i.d. were given orally (days 1-21). Chemotherapy was repeated every 28 days until a patient had received 6 cycles or progression was found. Twenty-nine patients were enrolled from October 2002 to December 2004. A total of 78 cycles were given at a mean of 2.7 cycles per patient. Response could be evaluated in 26 patients. Responses were partial remission in 4/26 patients (15.4%), stable disease in 8/26 (30.8%) and progression in 14/26 (53.8%). All patients progressed except for 2 in partial remission and 2 in stable disease. Toxicities could be assessed in 23 patients. Maximal hematological toxicities greater than grade 2 were leucopenia in 3 patients (11.5%), neutropenia in 2 (7.7%), anemia in 2 (7.7%), thrombocytopenia in 1 (3.8%) and febrile neutropenia in 3 (11.5%). Maximal nonhematological grade 3 or 4 toxicities were asthenia in 1 patient (3.8%), anorexia in 1 (3.8%), vomiting in 1 (3.8%), diarrhea in 2 (7.7%), allergic reaction in 1 (3.8%), hand-foot syndrome in 1 (3.8%) and hyperbilirubinemia in 1 (3.8%). All 29 patients were dead on last follow-up. Median progression-free survival was 3.91 months in 26 evaluable patients and median overall survival was 5.59 months in all patients. Combination chemotherapy including FDRI of gemcitabine seems minimally active for patients with advanced, nonoperable pancreatic cancer. Further research to improve effectiveness of chemotherapy for advanced pancreatic cancer is mandatory. 相似文献
63.
Lee BH Lee JH Lee SM Jeong SM Yoon IS Lee JH Choi SH Pyo MK Rhim H Kim HC Jang CG Lee BC Park CS Nah SY 《Neuropharmacology》2007,52(4):1139-1150
We previously demonstrated that 20(S)-ginsenoside Rg(3) (Rg(3)), one of the active components of Panax ginseng, non-competitively inhibits 5-HT(3A) receptor channel activity on extracellular side of the cell. Here, we sought to elucidate the molecular mechanisms underlying Rg(3)-induced 5-HT(3A) receptor regulation. We used the two-microelectrode voltage-clamp technique to investigate the effect of Rg(3) on 5-HT-mediated ion currents (I(5-HT)) in Xenopus oocytes expressing wild-type or 5-HT(3A) receptors harboring mutations in the gating pore region of transmembrane domain 2 (TM2). In oocytes expressing wild-type 5-HT(3A) receptors, Rg(3) dose-dependently inhibited peak I(5-HT) with an IC(50) of 27.6+/-4.3microM. Mutations V291A, F292A, and I295A in TM2 greatly attenuated or abolished the Rg(3)-induced inhibition of peak I(5-HT). Mutation V291A but not F292A and I295A induced constitutively active ion currents with decrease of current decay rate. Rg(3) accelerated the rate of current decay with dose-dependent manner in the presence of 5-HT. Rg(3) and TMB-8, an open channel blocker, dose-dependently inhibited constitutively active ion currents. The IC(50) values of constitutively active ion currents in V291A mutant receptor were 72.4+/-23.1 and 6.5+/-0.7microM for Rg(3) and TMB-8, respectively. Diltiazem did not prevent Rg(3)-induced inhibition of constitutively active ion currents in occlusion experiments. These results indicate that Rg(3) inhibits 5-HT(3A) receptor channel activity through interactions with residues V291, F292, and I295 in the channel gating region of TM2 and further demonstrate that Rg(3) regulates 5-HT(3A) receptor channel activity in the open state at different site(s) from those of TMB-8 and diltiazem. 相似文献
64.
Cho Han Joo Kim Jaemin Nah Seung Kwan Lee Jihyun Kim Chul Gu Kim Jong Woo 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》2022,260(3):839-848
Graefe's Archive for Clinical and Experimental Ophthalmology - To evaluate morphologic changes of choroidal neovascularization (CNV) on optical coherence tomography angiography (OCTA) during... 相似文献
65.
66.
Yun-Hoa Jung Bong-Hae Cho Kyung-Soo Nah Hyung-Il Kim Yong Hoon Kwon 《Lasers in medical science》2010,25(3):339-343
A diode-pumped solid state (DPSS) laser emitting at 473 nm was used to test its influence on the degree of polymerization
of composite resins. Eight composite resins were chosen and light cured with three different light-curing systems [a quartz–tungsten–halogen
(QTH) lamp-based unit, a light-emitting diode (LED) unit, and a DPSS laser]. Polymerization shrinkage and color change in
specimens were measured. According to the statistical analysis, each light-curing system produced a significantly different
value of maximum polymerization shrinkage. In most specimens, the DPSS laser induced the least polymerization shrinkage. After
being immersed in distilled water for 10 days, specimens light-cured by the DPSS laser had undergone less color change than
those cured by the other units. In conclusion, the DPSS laser induced better or similar polymerization in terms of polymerization
shrinkage and color change in composite resins compared with those of the QTH lamp-based and LED units. 相似文献
67.
Sung-Jae Kim Hong-Kyo Moon Sul-Gee Kim Yong-Min Chun Kyung-Soo Oh 《Clinical orthopaedics and related research》2010,468(4):1136-1141
It generally is believed generalized joint laxity is one of the risk factors for failure of anterior cruciate ligament (ACL)
reconstruction. However, no consensus exists regarding whether adverse effects on ACL reconstruction are attributable to joint-specific
laxity or are related to the severity of generalized joint laxity. We therefore asked whether knee stability and functional
outcomes would be related to joint-specific laxity and would differ according to the severity of generalized joint laxity.
The Beighton and Horan criteria were used to assess joint laxity in 272 subjects. All elements are added to give an overall
joint laxity score ranging from 0 to 5. Knee translation did not increase in proportion to the severity of the generalized
joint laxity. Patients with scores less than 4 showed similar knee stability. When all variables, including the severity of
generalized joint laxity, were considered, only hyperextension of the knee independently predicted knee stability and function.
In patients with knee hyperextension, a bone-patellar tendon-bone autograft provided superior stability and function compared
with a hamstring tendon autograft. Our data suggest knee hyperextension predicts postoperative stability and function regardless
whether patients have severe generalized joint laxity. 相似文献
68.
Chitosan-graft-polyethylenimine as a gene carrier. 总被引:16,自引:0,他引:16
Hu-Lin Jiang You-Kyoung Kim Rohidas Arote Jae-Woon Nah Myung-Haing Cho Yun-Jaie Choi Toshihiro Akaike Chong-Su Cho 《Journal of controlled release》2007,117(2):273-280
Chitosans have been proposed as biocompatible alternative cationic polymers that are suitable for non-viral delivery. However, the transfection efficiency of chitosan-DNA nanoparticles is still very low. To improve transfection efficiency, we prepared chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer by an imine reaction between periodate-oxidized chitosan and polyethylenimine (PEI). The molecular weight and composition of the CHI-g-PEI copolymer were characterized, using multi-angle laser scattering (GPC-MALS) and (1)H nuclear magnetic resonance ((1)H NMR), respectively. The copolymer was complexed with plasmid DNA (pDNA) in various copolymer/DNA (N/P) charge ratios, and the complex was characterized. CHI-g-PEI showed good DNA binding ability and high protection of DNA from nuclease attack. Also, with an increase in charge ratio, the sizes of the CHI-g-PEI/DNA complex showed a tendency to decrease, whereas the zeta potential of the complex showed an increase. The CHI-g-PEI copolymer had low cytotoxicity, compared to PEI 25K from cytotoxicity assays. At high N/P ratios, the CHI-g-PEI/DNA complex showed higher transfection efficiency than PEI 25K in HeLa, 293T and HepG2 cell lines. Our results indicate that the CHI-g-PEI copolymer has potential as a gene carrier in vitro. 相似文献
69.
Galactosylated chitosan-graft-polyethylenimine as a gene carrier for hepatocyte targeting 总被引:2,自引:0,他引:2
Jiang HL Kwon JT Kim YK Kim EM Arote R Jeong HJ Nah JW Choi YJ Akaike T Cho MH Cho CS 《Gene therapy》2007,14(19):1389-1398
Chitosans have been proposed as alternative, biocompatible cationic polymers for nonviral gene delivery. However, the low transfection efficiency and low specificity of chitosan need to be addressed before clinical application. We prepared galactosylated chitosan-graft-polyethylenimine (GC-g-PEI) copolymer by an imine reaction between periodate-oxidized GC and low-molecular-weight PEI. The molecular weight and composition were characterized using gel permeation chromatography column with multi-angle laser scattering and (1)H nuclear magnetic resonance, respectively. The copolymer was complexed with plasmid DNA in various copolymer/DNA (N/P) charge ratios, and the complexes were characterized. GC-g-PEI showed good DNA-binding ability and superior protection of DNA from nuclease attack and had low cytotoxicity compared to PEI 25K. GC-g-PEI/DNA complexes showed higher transfection efficiency than PEI 25K in both HepG2 and HeLa cell lines. Transfection efficiency into HepG2, which has asialoglycoprotein receptors, was higher than that into HeLa, which does not. GC-g-PEI/DNA complexes also transfected liver cells in vivo after intraperitoneal (i.p.) administration more effectively than PEI 25K. These results suggest that GC-g-PEI can be used in gene therapy to improve transfection efficiency and hepatocyte specificity in vitro and in vivo. 相似文献
70.