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Eileen M. Burd Mohammad Tauqeer Alam Karla D. Passalacqua Ameeta S. Kalokhe Molly E. Eaton Sarah W. Satola Colleen S. Kraft Timothy D. Read 《Journal of clinical microbiology》2014,52(8):3114-3117
Whole-genome sequencing was used to compare longitudinal isolates of Staphylococcus aureus that developed resistance to oxacillin (MIC up to 16 μg/ml). The mecA gene was absent. A novel 5-bp TATCC frameshift insertion in a gene encoding an ABC transporter similar to that of the teichoic acid translocation ATP-binding protein TagH and a 3-bp GCT nonframeshift insertion in the pdhA pyruvate dehydrogenase gene were detected in the oxacillin-resistant isolates. 相似文献
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Austin W. Chan Sarah Kabbani Gerald Staton Colleen S. Kraft 《Journal of clinical microbiology》2014,52(4):1281-1283
Mycobacterium paraffinicum has been newly recognized as a species. A case of symptomatic pulmonary infection caused by
M. paraffinicum is described, and as far as we know, this is the first case of the organism as a human pathogen. 相似文献
76.
Adam J. Oates Jonathan Sutcliffe Jens Stahlschmidt Jeannette K. Kraft 《Pediatric radiology》2014,44(1):112-114
A 15-year-old boy with caecal haematoma required a right hemicolectomy due to development of small bowel obstruction and near caecal perforation having presented several days after an episode of minor trauma. The position of the caecum between intraperitoneal and extraperitoneal bowel requires special treatment considerations. This is a unique case in an adolescent because caecal haematoma usually presents acutely with abdominal pain. 相似文献
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Donatella Spina Lorenzo Leoncini Tiziana Megha Maria T. Del Vecchio Chiara Minacci Simonetta Poggi Stefano Pileri Piero Tosi Rainer Kraft Jean A. Laissue Hans Cottier 《International journal of cancer. Journal international du cancer》1996,68(4):436-440
The present study examined whether growth characteristics of diffusely growing non-Hodgkin's lymphomas (NHL) may differ as a function of stage. Among 105 NHL of various types and sub-types (REAL [Revised European-American Lymphoma] classification), localized (Ann Arbor pathologic stages I + II) lymphomas exhibited clearly higher indices for mitotic activity, apoptosis and cell turnover, as well as a significantly lower percentage of cells containing immunohistochemically detectable bcl-2 protein, than disseminated (stages III + IV) NHL. A similar pattern emerged when high-grade (Kiel classification) lymphomas only were evaluated. Low-grade NHL showed analogous, but less marked, stage-dependent characteristics, with the exception of median percentages of bcl-2+ cells, which remained comparable in all stages. Our findings are consistent with the notion that dissemination of diffusely growing NHL is usually associated with reduced cell turnover and, in high-grade lymphomas, with the generation of longer-lived cells. © 1996 Wiley-Liss, Inc. 相似文献
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Expression of the VEP13 antigen (CD16) on native human alveolar macrophages and cultured blood monocytes 总被引:1,自引:0,他引:1
I Baumgartner O Scheiner C Holzinger G Boltz-Nitulescu H Klech H Lassmann H Rumpold O F?rster D Kraft 《Immunobiology》1988,177(3):317-326
Human alveolar macrophages (AM phi) from thirteen patients, who were suffering from various lung diseases were harvested by bronchoalveolar lavage. Peripheral blood monocytes from eight healthy donors were isolated by Ficoll-Hypaque gradient centrifugation and adherence to plastic surface. To detect the VEP13 antigen (CD16) on these cells, a rosette assay employing ox erythrocytes coated by the CrCl3 method with purified VEP13 monoclonal antibody (Eo-VEP13) was used. A mean of 31.3% of freshly isolated AM phi and 3.9% of blood monocytes formed Eo-VEP13 rosettes. Monocytes cultured for 3 or 6 days in the presence of a supernatant from mouse L929 cells, which had been shown previously to improve long-term viability of human monocytes in culture, showed 12.5% and 25.3% Eo-VEP13 rosettes, respectively. No significant increase in VEP13 antigen expression was noted by culturing monocytes without L929 cell supernatant. The factor in L929 supernatant that induces VEP13 antigen expression has not been identified. Tunicamycin at 10 micrograms/ml inhibited significantly VEP13 antigen expression on monocytes. In contrast, IgG rosette formation was not reduced by tunicamycin. Our data show that subpopulations of native human AM phi and peripheral blood monocytes cultured in presence of a supernatant of L929 fibroblasts containing mainly murine CSF may express the CD16 antigen, which is normally found on large granular lymphocytes (LGL). Suppression by tunicamycin indicates that Fc receptor glycosylation takes place during a later differentiation step of mononuclear phagocytes. 相似文献
80.
Wolfram Dietz Regina Montag Ulrike Kraft Mario Walther Bernd W. Sigusch Peter Gaengler 《Journal of dentistry》2014