全文获取类型
收费全文 | 174篇 |
免费 | 9篇 |
国内免费 | 46篇 |
专业分类
儿科学 | 7篇 |
妇产科学 | 6篇 |
基础医学 | 26篇 |
口腔科学 | 1篇 |
临床医学 | 44篇 |
内科学 | 29篇 |
皮肤病学 | 4篇 |
神经病学 | 2篇 |
特种医学 | 43篇 |
外科学 | 16篇 |
综合类 | 15篇 |
预防医学 | 5篇 |
眼科学 | 5篇 |
药学 | 19篇 |
肿瘤学 | 7篇 |
出版年
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 2篇 |
2014年 | 2篇 |
2013年 | 8篇 |
2012年 | 7篇 |
2010年 | 8篇 |
2009年 | 11篇 |
2008年 | 5篇 |
2007年 | 28篇 |
2006年 | 9篇 |
2005年 | 11篇 |
2004年 | 4篇 |
2003年 | 3篇 |
2002年 | 1篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 14篇 |
1997年 | 18篇 |
1996年 | 5篇 |
1995年 | 10篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 3篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1976年 | 6篇 |
1975年 | 3篇 |
排序方式: 共有229条查询结果,搜索用时 93 毫秒
61.
Baker HW 《国外医学(计划生育.生殖健康分册)》2010,29(3):225-226,238
陆金春等^[1]所著的“中国118家实验室精液分析状况的调查”一文主要回顾了当前在中国采用的精液分析方法。调查使用的是作者自己设计的包括36个问题的“男科实验室精液分析调查表”。给145家精液实验室发放了调查表,收回118份答卷。调查表均由实验室专业技术人员填写。 相似文献
62.
Milani Junior R; Jorge MT; de Campos FP; Martins FP; Bousso A; Cardoso JL; Ribeiro LA; Fan HW; Franca FO; Sano-Martins IS; Cardoso D; Ide Fernandez C; Fernandes JC; Aldred VL; Sandoval MP; Puorto G; Theakston RD; Warrell DA 《QJM : monthly journal of the Association of Physicians》1997,90(5):323-334
The jararacucu, one of the most dreaded snakes of Brazil, southern Bolivia,
Paraguay and northeastern Argentina, is a heavily-built pit viper which may
grow to a length of 2.2 m. Up to 1000 mg (dry weight) of highly-lethal
venom may be milked from its venom glands on a single occasion. It has
accounted for 0.8% to 10% of series of snake bites in Sao Paulo State,
Brazil. We examined 29 cases of proven jararacucu bites recruited over a
20-year period in two Sao Paulo hospitals. Severe signs of local and
systemic envenoming, (local necrosis, shock, spontaneous systemic bleeding,
renal failure) were seen only in patients bitten by snakes longer than 50
cm; bites by shorter specimens were more likely to cause incoagulable
blood. Fourteen patients developed coagulopathy, six local necrosis
(requiring amputation in one) and five local abscesses. Two became shocked
and four developed renal failure. Three patients, aged 3, 11 and 65 years,
died 18.75, 27.75 and 83 h after being bitten, with respiratory and
circulatory failure despite large doses of specific antivenom and
intensive-care- unit management. In two patients, autopsies revealed acute
renal tubular necrosis, cerebral oedema, haemorrhagic rhabdomyolysis at the
site of the bite and disseminated intravascular coagulation. In one
survivor with chronic renal failure, renal biopsy showed bilateral cortical
necrosis; the patient remains dependent on haemodialysis. Effects of
polyspecific Bothrops antivenom were not impressive, and it has been
suggested that anti-Bothrops and anti-Crotalus antivenoms should be given
in combination.
相似文献
63.
在发明和常规应用卵胞浆内单精子注射(ICSI)之前,十多年的临床体外受精(IVF)治疗实践中,受精率低下很常见,大约有20%~35%的IVF患者受精率很低(〈35%的卵子受精)和受精完全失败(所有卵子都不受精)。虽然受精失败与精子或卵子的质量有关,但相当一部分患者受精失败与精液质量或精子功能低下有很密切相关性。最常见的是严重的少精,弱精和畸形精子症患者。 相似文献
64.
RCA Schellekens GG Olsder SMCH Langenberg T Boer HJ Woerdenbag HW Frijlink JGW Kosterink F Stellaard 《British journal of pharmacology》2009,158(2):532-540
Background and purpose:
13C-urea may be a suitable marker to assess the in vivo fate of colon-targeted dosage forms given by mouth. We postulated that release in the colon (urease-rich segment) of 13C-urea from colon-targeted capsules would lead to fermentation of 13C-urea by bacterial ureases into 13CO2. Subsequent absorption into the blood and circulation would lead to detectable 13C (as 13CO2) in breath. If, however, release of 13C-urea occurred in the small intestine (urease-poor segment), we expected detectable 13C (as 13C-urea) in blood but no breath 13C (as 13CO2). The differential kinetics of 13C-urea could thus potentially describe both release kinetics and indicate the gastrointestinal segment of release.Experimental approach:
The in vivo study consisted of three experiments, during which the same group of four volunteers participated.Key results:
The kinetic model was internally valid. The appearance of 13C-in breath CO2 (Ffermented) and the appearance of 13C in blood as 13C-urea (Fnot fermented) show a high inverse correlation (Pearson''s r=−0.981, P= 0.06). The total recovery of 13C (Ffermented+Fnot fermented) averaged 99%, indicating complete recovery of the administered 13C via breath and blood. 13CO2 exhalation was observed in all subjects. This indicates that 13C-urea was available in urease-rich segments, such as the caecum or colon.Conclusions and implications:
In this proof-of-concept study, 13C-urea was able to provide information on both the release kinetics of a colon-targeted oral dosage form and the gastrointestinal segment where it was released. 相似文献65.
Manson MM; Ball HW; Barrett MC; Clark HL; Judah DJ; Williamson G; Neal GE 《Carcinogenesis》1997,18(9):1729-1738
A range of potential chemoprotective agents, most of them natural dietary
constituents, has been examined for ability to modulate both phase I
(cytochrome P450 1A1, 1A2, 2B1/2, 2C11, 2E1, 3A, 4A) and phase II drug
metabolizing enzymes (glutathione S-transferases, in particular subunits
Yc2 and P, aflatoxin B1-aldehyde reductase and quinone reductase) in rat
liver. In addition to assays of total enzyme activity and Western blots for
individual isozymes, the ability of microsomes to metabolize aflatoxin B1,
and of cytosols to conjugate aflatoxin B1 (AFB1)-epoxide to GSH and to
produce AFB1-dialcohol, were measured. Induction of gamma-glutamyl
transpeptidase activity was examined by histochemistry. Differing patterns
of induction were observed, reflecting differences in the control of
expression of the individual enzymes studied. Of the compounds examined,
butylated hydroxytoluene, ethoxyquin, indole-3-carbinol and phenethyl
isothiocyanate were the most potent bifunctional agents (inducing both
phase I and II activities). Oltipraz, while only weakly inducing CYP1A2 and
2B1/2, was a potent inducer of phase II enzymes. Caffeic acid, garlic oil,
sinigrin and propyl gallate all showed some ability to induce phase II
enzymes. 4-Methyl catechol, alpha-tocopherol and red wine decreased certain
phase I enzyme activities, while inducing total GST activity. Butylated
hydroxytoluene, ethoxyquin, garlic oil and indole-3-carbinol induced gamma
glutamyltranspeptidase in periportal hepatocytes. Particularly because of
their ability to induce the detoxifying activities of glutathione
S-transferase Yc2 and aldehyde reductase, butylated hydroxytoluene,
ethoxyquin, indole-3-carbinol, oltipraz, phenethyl isothiocyanate and
sinigrin will be effective blocking agents in rodents, if administered
prior to AFB1. While these studies indicate the relative contributions of
phase I and II metabolism in the overall protective effect in rat, care
should be taken that a similar balance is achieved in man, and that
relevant enzymes or iso forms are induced.
相似文献
66.
Insulin-like growth factor (IGF)-I and IGF binding protein-3 concentrations in fluid from human stimulated follicles 总被引:2,自引:5,他引:2
Oosterhuis GJ; Vermes I; Lambalk CB; Michgelsen HW; Schoemaker J 《Human reproduction (Oxford, England)》1998,13(2):285-289
Insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP- 3)
play an important role in regulating follicle growth and maturation. We
have evaluated whether responsiveness to gonadotrophins during an in- vitro
fertilization (IVF) treatment is related to follicular fluid IGF- I and
IGFBP-3 concentrations. We also investigated if a difference is present in
IGF-I and IGFBP-3 concentrations between patients treated with human
menopausal gonadotrophin (HMG) and patients treated with highly purified
follicle stimulating hormone (FSH). We have measured IGF-I and IGFBP-3 in
follicular fluid from pre-ovulatory follicles in an IVF programme. All 70
patients were stimulated after being down- regulated with a
gonadotrophin-releasing hormone (GnRH) analogue. IGF-I concentrations in
follicular fluid were significantly inversely correlated with the number of
ampoules FSH administered and number of days of FSH administration, and
significantly correlated with the number of follicles aspirated. IGFBP-3
concentrations were not correlated with any other parameter measured nor
were IGF-I and IGFBP-3 concentrations correlated. IGFBP-3 concentrations
were significantly higher in patients receiving highly purified FSH
compared with patients receiving HMG (P < 0.005). These results are new
evidence that IGF-I concentration in follicular fluid is higher in women
who respond better to follicular stimulation, i.e. women who grow many
follicles, women who need a shorter duration of stimulation and women who
need fewer ampoules FSH before oocyte retrieval.
相似文献
67.
Immunosuppressive activities in the seminal plasma of infertile men: relationship to sperm antibodies and autoimmunity 总被引:3,自引:1,他引:3
Imade GE; Baker HW; de Kretser DM; Hedger MP 《Human reproduction (Oxford, England)》1997,12(2):256-262
Semen samples from infertile men were assessed for sperm autoimmunity by
direct immunobead assay for immunoglobulin (Ig)A and IgG sperm antibodies
and mucus penetration test. Immunosuppressive activity in seminal plasma
was measured by an in-vitro bioassay employing dose- dependent inhibition
of phytohaemagglutinin-induced activation of rat thymocytes, in the
presence or absence of hydroxylamine (0.1 mM), an inhibitor of polyamine
oxidation. All seminal plasma samples, regardless of autoimmune status,
caused inhibition of T-lymphocyte activation, and hydroxylamine reduced
this bioactivity by appproximately 50%. Dialysis (<3500 molecular
weight) also significantly reduced seminal plasma bioactivity, both in the
presence and absence of hydroxylamine. In the presence of hydroxylamine,
there was a negative correlation between IgA, but not IgG, antibody
concentrations and lymphosuppressive activity in seminal plasma.
Antibody-positive samples displaying impaired sperm function, as indicated
by the mucus penetration test, had reduced activity compared with other
samples. In contrast, there was no relationship between sperm autoimmunity
and lymphosuppressive activity assayed in the absence of hydroxylamine. The
data indicate that T-lymphocyte inhibition by human seminal plasma is due
to multiple factors, and reduced amounts of these factors may contribute to
the development and/or persistence of sperm autoimmunity in infertile men;
however, differences in polyamine substrates available for oxidation in
semen do not appear to be a major contributing factor.
相似文献
68.
0 引言 肝炎后高胆红素血症 ,极易与肝炎伴发高胆红素血症相混淆 .该病是肝炎后遗症之一 ,临床上并不少见 .患者常以间歇性黄疸或隐性黄疸前来就诊 ,但并无先天性黄疸、肝炎、肝硬化及肝内外胆管结石的临床证据 ,多数既往有肝炎病史 ,肝炎已达治愈标准 ,虽增加活动量亦无复发 ,临床预后良好 .现将我院 1995 - 10 / 1999- 0 9肝炎后高胆红素血症 18例作一小结 .1 临床资料 男 12例 ,女 6例 ,年龄 2 2~ 5 8(平均 40岁 ) .门诊 11例 ,住院 7例 .其中 11例曾患急性病毒性乙型肝炎 ,1例曾患中毒性肝炎 ,1例曾患药物性肝炎 ,5例无明确肝炎病… 相似文献
69.
血红素氧合酶-1在器官移植中的保护作用 总被引:4,自引:2,他引:2
血红素氧合酶(heme oxygenase,HO)是体内唯一一种催化血红素分解代谢的限速酶,他可以氧化降解血红素,将其分解为一氧化碳、自由铁和胆绿素.血红素氧合酶-1(HO-1)是唯一可以被诱导的血红素氧合酶,近年来大量的研究发现HO-1具有抗炎、抗凋亡、抗增生反应等多种保护作用.HO-1不仅可以在机体生理状态下发挥作用,更为重要的是他可以在机体非正常状态包括应激状态下被诱导,被认为是在细胞受损时维持其氧化和抗氧化动态平衡的关键因素.本文就HO-1在器官移植研究领域的细胞保护作用作如下综述. 相似文献
70.
Alina Hilger Charlotte Schramm Tracie Pennimpede Lars Wittler Gabriel C Dworschak Enrika Bartels Hartmut Engels Alexander M Zink Franziska Degenhardt Annette M Müller Eberhard Schmiedeke Sabine Grasshoff-Derr Stefanie M?rzheuser Stuart Hosie Stefan Holland-Cunz Charlotte HW Wijers Carlo LM Marcelis Iris ALM van Rooij Friedhelm Hildebrandt Bernhard G Herrmann Markus M N?then Michael Ludwig Heiko Reutter Markus Draaken 《European journal of human genetics : EJHG》2013,21(12):1377-1382
The acronym VATER/VACTERL association describes the combination of at least three of the following congenital anomalies: vertebral defects (V), anorectal malformations (A), cardiac defects (C), tracheoesophageal fistula with or without esophageal atresia (TE), renal malformations (R), and limb defects (L). We aimed to identify highly penetrant de novo copy number variations (CNVs) that contribute to VATER/VACTERL association. Array-based molecular karyotyping was performed in a cohort of 41 patients with VATER/VACTERL association and 6 patients with VATER/VACTERL-like phenotype including all of the patients'' parents. Three de novo CNVs were identified involving chromosomal regions 1q41, 2q37.3, and 8q24.3 comprising one (SPATA17), two (CAPN10, GPR35), and three (EPPK1, PLEC, PARP10) genes, respectively. Pre-existing data from the literature prompted us to choose GPR35 and EPPK1 for mouse expression studies. Based on these studies, we prioritized GPR35 for sequencing analysis in an extended cohort of 192 patients with VATER/VACTERL association and VATER/VACTERL-like phenotype. Although no disease-causing mutation was identified, our mouse expression studies suggest GPR35 to be involved in the development of the VATER/VACTERL phenotype. Follow-up of GPR35 and the other genes comprising the identified duplications is warranted. 相似文献