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101.
Mr N. Ishikawa S. Suda T. Sasaki T. Yamanishi H. Hosaka K. Yasuda H. Ito 《Medical & biological engineering & computing》1998,36(6):704-710
A system composed of a functional continuous magnetic stimulator (FCMS) and a saddle-type coil has been developed for non-invasive
treatment of urinary incontinence, especially stress incontinence and urge incontinence. The FCMS conditions were as follows:
2 kW maximum electrical power consumption, 800 V maximum capacitor voltage, 720 μs pulsewidth (180 μs rise time), and 5–30
Hz frequency. A frequency between 5 and 10 Hz is used to treat urge incontinence and a frequency between 25 Hz and 30 Hz is
used to treat urge incontinence. The coil (120 mm long, 90 mm wide and 50 mm thick) fits the most suitable region for this
treatment, the region from the anus to the perineum. The coil is cooled to maintain a coil temperature between 20 and 25°C
so that it can be used efficiently and safely. In experiments with anaesthetised dogs, it was confirmed that the urethral
pressure increased when the circumference of the perineum received continuous magnetic stimulation of 720 μs pulsewidth (180
μs rise time), 10Hz frequency and about 520 V capacitor voltage. This result suggests that magnetic stimulation can be effective
as a urinary incontinence therapy. 相似文献
102.
Kazuo Takayama Kanichi Yagawa Akiko Takahashi Hironori Nishio Morio Sudo Nobuyuki Sasaki Toru Yoshida Masanobu Satoh Hisaaki Abiko Akio Nunokawa Mitsuo Okazaki 《Pathology international》1969,19(4):547-562
A typical case of the D uchenne type of progressive muscular dystrophy with autopsy findings was presented. Changes in the myocardial and smooth muscle of many organs were found, and the skeletal muscles also revealed florid changes.
Histopathological examination of the skeletal muscle was made in detail through light and electron microscopic observation. 相似文献
Histopathological examination of the skeletal muscle was made in detail through light and electron microscopic observation. 相似文献
103.
104.
105.
Transforming growth factor beta is protective in host resistance against Listeria monocytogenes infection in mice. 下载免费PDF全文
A Nakane M Asano S Sasaki S Nishikawa T Miura M Kohanawa T Minagawa 《Infection and immunity》1996,64(9):3901-3904
The role of transforming growth factor beta (TGF-beta) in host resistance against Listeria monocytogenes infection was studied with mice. The constitutive expression of TGF-beta 1 mRNA was observed in the spleens and livers of mice before and after infection. Injecting the mice with anti-TGF-beta 1 peptide serum resulted in diminished antilisterial resistance, whereas the administration of human platelet-derived TGF-beta 1 enhanced the resistance. Moreover, mice were protected against lethal infection when treated with TGF-beta 1. These results suggest the TGF-beta 1 might be involved in antilisterial resistance. On the other hand, injecting the mice with TGF-beta 1 resulted in a decrease in the titers of endogenous gamma interferon, tumor necrosis factor alpha, and interleukin-6, which are crucial in antilisterial resistance, in sera and in extracts of spleen and liver. Thus, a complicated mechanism might be involved in the role of TGF-beta 1 in host resistance against L. monocytogenes infection. 相似文献
106.
Cross-linking of Fc(gamma)-receptor on monocytes inhibits hepatitis C virus-specific cytotoxic T-lymphocyte induction in vitro. 总被引:2,自引:0,他引:2 下载免费PDF全文
T Kanto N Hayashi T Takehara K Katayama A Ito K Mochizuki N Kuzushita T Tatsumi Y Sasaki A Kasahara M Hori 《Immunology》1998,94(4):461-468
In hepatitis C virus (HCV) infection, immune complex (IC)-type virus particles are frequently observed in circulation. The IC leads to cross-linking of Fcgamma receptors (FcgammaR) on monocytes and exerts immunoinhibitory function. To test the roles of IC in HCV-specific cytotoxic T lymphocyte (CTL) induction, we generated HCV CTL from peripheral blood mononuclear cells of chronic hepatitis C patients with or without HCV-IC- or immunoglobulin G (IgG)-coated culture plates and compared their lytic activities. HCV-IC or adherent IgG, which induces FcgammaR cross-linking, significantly reduced CTL activity. Expression of B7-1 on monocytes decreased on adherent IgG. In addition, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1 (TGF-beta1) production increased from cells on adherent IgG and their mRNA expression in monocytes was enhanced. Anti-TNF-alpha antibody during induction on adherent IgG inhibited lysis; however, anti-TGF-beta completely reversed its inhibitory effect. These results demonstrated that HCV-IC or adherent IgG impaired HCV-CTL induction in vitro. The FcgammaR-mediated CTL suppression occurred via decreased expression of monocyte B7-1 and/or enhanced production of TGF-beta1. 相似文献
107.
Direct tubular effects of arginine vasopressin (AVP) on water and NaCl transport across the medullary thick ascending limb of Henle (MAL) were examined by the in vitro perfusion of isolated nephron fragments of mice, rats, and rabbits. Osmotic water permeability of the MAL of mice and rats was low and remained unchanged with 2 mU/ml AVP added to the bath. A dose-dependent increase in transepithelial electrical potential difference (PD) with AVP was observed in the mouse MAL when the ambient medium was isotonic. A similar result was also obtained when 2×10–4 mol/l dibutyryl adenosine 3,5-cyclic-monophosphate was added to the bath. In this preparation, AVP also caused an increase in the unidirectional Cl efflux from 323±45 to 398±61 pmoles·mm–1 ·min–1 (n=6,P<0.05). In contrast, under similar condition, we could not demonstrate any effect of AVP on PD, Cl efflux, or net Na flux in the rat MAL and on PD and Cl efflux in the rabbit MAL. Both PD and Cl efflux in the rat MAL were unaffected by AVP when the perfusate was made hypotonic. However, when the ambient medium was made hypertonic by adding NaCl and urea, a significant increase in PD was observed. In addition, we confirmed that AVP stimulated adenylate cyclase activity in the MAL as well as in the collecting tubule of mice and rats. We conclude that AVP stimulates Cl transport across the MAL of mice and rats by activating adenylate cyclase-cyclic AMP system. However, this effect of AVP may quantitatively vary among species. 相似文献
108.
Proliferative activity of intratumoral fibroblasts is closely correlated with lymph node and distant organ metastases of invasive ductal carcinoma of the breast 总被引:3,自引:0,他引:3 下载免费PDF全文
Mitotic figures of fibroblasts are seen within invasive ductal carcinoma (IDC) of the breast. This suggests that the proliferative activity of fibroblasts may play an important role in IDC tumor progression. The purpose of this study was to examine whether the proliferative activity of fibroblasts can predict lymph node metastasis (LNM) or distant-organ metastasis (DOM) of IDCs. Two hundred four consecutive patients with IDC of the breast surgically treated at the National Cancer Center Hospital East constituted the basis of this study. Proliferative activity of fibroblasts was immunohistochemically evaluated by the mouse MIB-1 monoclonal antibody against Ki-67 antigen. The MIB-1 labeling index was the percentage of fibroblasts with positively stained nuclei, and fields for cell counting were selected in inner and outer areas within IDCs. In both areas, 300 fibroblasts were counted in each high-power field. The significance of proliferative activity of fibroblasts on LNM or DOM was compared with well-known prognostic parameters. Multivariate analyses demonstrated that a MIB-1 labeling index of more than 10% of fibroblasts in the inner area of IDCs significantly increased the relative risk of LNM and hazard rate of DOM (P < 0.001 and P = 0.007, respectively). The present study indicated that the metastatic ability of IDCs is closely dependent on proliferative activity of fibroblasts in the inner area. 相似文献
109.
110.
Biological properties and gene expression associated with metastatic potential of human osteosarcoma 总被引:4,自引:0,他引:4
Nakano T Tani M Ishibashi Y Kimura K Park YB Imaizumi N Tsuda H Aoyagi K Sasaki H Ohwada S Yokota J 《Clinical & experimental metastasis》2003,20(7):665-674
Lung metastasis has a great influence on the prognosis of patients with osteosarcoma. We previously established two high-metastatic
sublines, M112 and M132, from the HuO9 human osteosarcoma cell line by in vivo selection. In this study, we newly isolated a high-metastatic subline, H3, and three low-metastatic sublines, L6, L12 and
L13, from HuO9 by the dilution plating method. Three high-metastatic sublines produced more than 200 metastatic nodules in
the lung, while three low-metastatic sublines produced no or few nodules after injection of 2 × 106 cells into the tail vein of nude mice. There were significant differences in the motility and invasiveness between high-
and low-metastatic sublines, whereas the growth rates in vitro and the tumorigenicity in vivo showed no correlation with their metastatic abilities. Early adherence to culture plates was significantly lower in two of
three low-metastatic sublines, which occupied smaller surface areas on the culture plates than other sublines did. Comparison
of the expression of 637 cancer-related genes by cDNA microarray revealed that seven genes were differentially expressed between
high- and low-metastatic sublines. Among them, five genes (AXL, TGFA, COLL7A1, WNT5A, and MKK6) were associated with adherence, motility, and/or invasiveness. These results suggest that the differences in motility/invasiveness
and adhesive abilities are key determinants of lung metastasis in osteosarcoma.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献