Spinal pelvic reconstruction after total sacrectomy for en bloc resection of a giant sacral chordoma. Technical note

Although radical resection prolongs the disease-free survival period, surgical management of primary sacral tumors is challenging because of their location and often large size. Moreover, in cases of lesions for which a radical resection necessitates total sacrectomy, reconstruction is required. The authors have previously described a modified Galveston technique in which a liaison between the spine and pelvis is achieved using lumbar pedicle screws and Galveston rods embedded into the ilia; additionally, a transiliac bar reestablishes the pelvic ring. Although this reconstruction technique achieves stabilization, several biomechanical limitations exist. In the present report the authors present the case of a patient who underwent spinal pelvic reconstruction after a total sacrectomy was performed to remove a giant sacral chordoma. They describe a novel spinal pelvic reconstruction technique that addresses some of the biomechanical limitations.     

5/6肾切除大鼠早期模型中p38MAPK的动态变化

目的：研究5/6肾切除大鼠模型中p38MAPK磷酸化的动态表达情况。方法：82只雄性SD大鼠分为两组：5/6肾切除模型组72只,假手术对照组10只。模型组造模完成后,分别于肾切除早期（术后1/2h、1h、3h、6h、12h、1d、2d、4d）相应时间点处死,每组9只,假手术组于术后12h处死,称量各组体重及残余肾重,计算肥大指数;下腔静脉取血留取血清测定血肌酐（Scr）、尿素氮（BUN）;以Western blotting免疫印迹法检测肾皮质磷酸化p38MAPK活性的表达情况;以病理光镜和电镜观察肾小球、肾小管及肾间质组织形态学及超微结构的变化。结果：模型组与对照组相比,肾脏呈代偿性肥大,肥大指数增高（P〈0.05）,Scr和BUN升高（P均〈0.01）。免疫印迹法显示5/6肾切除术后1/2h、1h、3h、6h、12h磷酸化p38信号呈递增趋势,尤以12h信号最强,之后信号减弱,2d时几乎不可见,4d有较弱的信号,假手术组未见明显信号。光镜下,发现肾小球有轻度系膜细胞增生,基质增宽不明显,肾小管偶有轻度变性,间质偶有炎性细胞浸润。电镜下,偶见足突局灶融合。结论：5/6肾切除大鼠在造模完成后12h时磷酸化p38MAPK表达最强。     

A simple and economical method for the manual construction of frozen tissue arrays

Tsao S‐C, Wu C‐C, Wen C‐H, Huang Y‐C, Chai C‐Y. A simple and economical method for the manual construction of frozen tissue arrays. APMIS 2010; 118: 739–43. Tissue microarray has been developed to enable multiple cores of tissue in one or more new paraffin blocks. Currently, almost all tissue microarrays are made by coring cylindrical tissues from formalin‐fixed and paraffin‐embedded tissues. The disadvantages of formalin‐fixed and paraffin‐embedded tissues include the poor preservation of antigenicity of certain proteins and mRNA degradation induced by the fixation and embedding process. However, frozen tissue array construction presents technical difficulties, and tissue array devices are expensive, particularly for small‐ and medium‐sized laboratories. We describe a simple manual method for producing well‐aligned tissue arrays by a capsule freeze method that allows us to successfully perform hematoxylin–eosin and immunohistochemical stain. All 120 tissue samples were collected and constructed into blocks by this capsule freeze method. The capsules were not affected during the sectioning process, and the capsule material always disappeared during the aqueous steps of the stain processing. The frozen tissue arrays were smoothly sectioned without the use of a tape transfer system and immunohistochemical study was performed with satisfactory results. This alternative method can be applied in any laboratory, and is both simple and economical.     

完善高级专业技术资格评定工作的若干探讨

本文针对当前职称评审工作中出现的新形势、新情况和新要求，介绍了我校近年来高级专业技术资格评定工作的改革与实践，对于完善高级专业技术资格评定工作具有较好的参考价值。     

良好保存血管在体形态取材的一种方法

０　引言　我们观察了动脉血管的固定方法（浸泡固定或灌注固定）、固定时血管的生理状态（处于收缩或舒张）等因素对保存血管在体形态的影响．１　材料和方法１．１　材料　雄性ＳＤ大鼠（体质量２５０ｇ～３００ｇ）９只．动物被随机分为３组（每组３只），分别用于浸泡固定法（Ｉ组）、生理压力下４０ｍＬ／Ｌ多聚甲醛灌注固定（Ｓ组）、低压下Ｋｒｅｂｓ液预灌注４０ｍＬ／Ｌ多聚甲醛灌注固定（Ｄ组）对血管在体形态的影响的观察．动物饲养与实验均遵照第四军医大学实验动物饲养与使用规定进行．１．２　方法　３组动物均采用腹腔内注射…     

类胰岛素样生长因子-1和抑制素与多囊卵巢综合征相关性分析

目的 为探讨类胰岛素生长因子（IGF-1）和抑制素（INH）在多囊卵巢综合征（PCOS）发病中的作用。方法 检测30例PCOS患者血清类胰岛素生长因子-1（IGF-1）、空腹胰岛素（IN）。抑制素（INH），瘦素（Leptin），黄体生成素（Ln）、卵泡刺激素（FSH）、人体催乳素（PRL）、雌二醇（E2）和睾酮（T）。并取20例排卵正常、无怀孕妇女进行对照。结果 PCOS组IGF-1、IN、INH、LEP、T、LH均高于正常对照组，并有显著性意义（P〈0．05）。且IGF-1与INH呈正相关（r=0．7194，P〈0．01），INH与T也呈正相关（r=0．4343,P〈0．05）。FSH、PRL、E2随均低于正常对照组。结论 IGF-1和INH在多囊卵巢综合征（PCOS）中异常升高。     

贝氏柯克斯体的分子生物学进展

Q热是由贝氏柯克斯体引起的呈世界性分布的一种人兽共患疾病。目前，世界反恐组织已将该病病原体列为生物战剂之一。早期应用抗生素对Q热有疗效,而慢性Q热治疗周期长、易复发,死亡率高。贝氏柯克斯体能以气溶胶的形式传播,一旦流行将难以控制，因此对人类危害极大。为进一步研究该病的致病机制、及时有效的诊断手段及其疫苗的开发提供参考依据，本文就贝氏柯克斯体的分子生物学研究进展作简要概述。