脑卒中患者对负性情绪及心理治疗认知状况的调查

目的：调查甘肃省脑卒中患者对负性情绪是否影响肢体功能以及心理治疗的认知。方法：采用问卷调查方式,对甘肃省10家不同级别的省市县级医院神经内、外科2010年收治的脑卒中住院患者1 100例进行调查。结果：负性情绪对肢体功能恢复的影响占69.80%,脑卒中后心理治疗的需要占76.99%。结论：负性情绪对脑卒中患者肢体功能恢复有影响,并且非常需要心理治疗。     

硼替佐米作用不同时间对K562耐药细胞株核因子-κB途径的影响

 目的　观察硼替佐米（商品名：万珂,PS-341）对柔红霉素（DNR）诱导的K562耐药细胞株（K562／DNR）核因子-κB（NF-κB）、抑制蛋白κB（IκB）及P-糖蛋白（P-gp）表达的影响,探讨PS-341逆转耐药的分子机制。方法　以100 μg／ml DNR单用或联合应用4 μg／L PS-341分别作用于K562／DNR 12、24及36 h,检测不同时间点各组NF-κB、IκB及P-gp表达情况,同时测定NF-κB p65活性,检测各组细胞凋亡率。结果　Western blot结果显示：与阴性对照组相比,DNR可诱导NF-κB表达上调及活性增强、IκB表达下调、P-gp表达上调;加用PS-341可显著抑制 DNR诱导的NF-κB及P-gp表达,使IκB表达增加。加用PS-341后,NF-κB活性12 h为（15.3±1.87）%[DNR组为（23.8±2.27）%],24 h为（10.2±1.69）%[DNR组为（25.4±1.98）%],36 h为（6.08±2.53）%[DNR组为（26.9±2.58）%],与相应单用DNR组相比均有明显下降,差异有统计学意义（P值均＜0.05）。DNR与PS-341联用后,细胞凋亡率12 h为（35.23±5.15）%[DNR组为（15.56±4.12）%],24 h为（40.26±6.89）%[DNR组为（17.25±2.89）%],36 h为（43.58±7.69）%[DNR组为（22.47±4.58）%],与DNR组相比,细胞凋亡率均明显增加,差异具有统计学意义（P值均＜0.05）。上述作用呈时间依赖性。结论　PS-341可减少K562／DNR细胞NF-κB的活化,降低P-gp表达,逆转细胞耐药,促进细胞凋亡。     

经尿道等离子双极电切术治疗良性前列腺增生(附80例报告)

目的 探讨经尿道前列腺等离子双极电切术(PKRP)治疗前列腺增生(BPH)的疗效.方法 采用PKRP治疗BPH80例,随访1～10个月.结果 80例BPH患者均成功手术.术中止血效果满意,无前列腺电切综合征(TURS).国际前列症状评分(I-PSS)由(27.4±3.2)分降至(8.1±4.2)分.最大尿流率(Qmax)由(6.1±1.9)ml/s升至(23.7±3.1)ml/s.结论 PKRP治疗BPH具有创伤小、安全、恢复快、并发症少,是一种治疗BPH的理想方式.     

自拟中药方浴足治疗老年闭塞性下肢动脉硬化症

【目的】探讨中药浴足治疗闭塞性下肢动脉硬化症的临床疗效。【方法】120例患者随机分为治疗组和对照组,对照组给予丁咯地尔10mg,每日一次静滴,治疗组在此基础上加用自拟中药方煎煮浴足,每日一次,于治疗前后,第1、2周末对足疼痛发作频次、持续时间、疼痛程度和踝肱指数（ABI）进行测评。【结果】加用中药浴足组在减少足静息痛发作频次、缩短每次发作持续时间、减轻疼痛程度、改善ABI及降低复发方面,均明显优于单用丁咯地尔组。【结论】中药浴足加丁咯地尔治疗闭塞性下肢动脉硬化症有较好疗效。     

舒平烧伤膏治疗烧烫伤1358例

目的：观察舒平烧伤膏治疗烧烫伤的,临床疗效。方法：应用局部涂摸、暴露或弹力绷带加压包扎的方法,治疗各种烧烫伤患者1358人。结果：伤口愈合率100％,烧伤创面平均愈合时间浅II度（9．30±5．28）天,深II度为（16．87±6．63）天,III度为（33．80±17．46）天。烧伤深度越深、程度越重、住院应用抗生素的患者平均医疗费用和每1％面积医疗费用越高。结论：舒平烧伤膏治疗烧烫伤效果显著。     

Effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 （HSV-1）

To observe the effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 (HSV-1) so as to explore the mechanism of its antiviral activity, fluorescein isothiocyanate (FITC) was used as the fluorescent probe to label viruses and heparin sodium was used as control. Meanwhile , the effect of Gardenia extract ZG on the adsorption quantity on the surface of Hep-2 cells was determined by flow cytometry. It was demonstrated that adsorption of HSV-1 on the surface of Hep-2 cells exhibited the character of saturation and specificity and heparin sodium could prevent attachment of viruses on these cells. These results are in accord with those reported previously. It was also proved that the manner of drug-use prior to adsorption or simultaneous use of drug and adsorption was better than adsorption prior to drug-use, and the inhibition rates of the former and latter manner were 84. 76% and 82.92% respectively. Three manners of drug-use with Gardenia extract ZG were all effective to reduce the adsorption quantity of viruses, especially the manner of simultaneous use of drug and adsorption with an adsorption inhibition rate of 68.46% . From the above observation, it is apparent that the mechanism of anti-viral activity of Gardenia extract ZG may be via several steps involved in the HSV-1 adsorption.     

耳大神经及腮腺筋膜解剖的再认识与腮腺切除手术的改良

目的：对耳大神经及腮腺筋膜解剖进行再认识，由此改良腮腺切除手术方法。方法：解剖成人尸体10侧，术中活体解剖20侧，对耳大神经和腮腺筋膜的解剖要素进行观察。根据观察结果进行改良腮腺切除术14例，即在腮腺筋膜表面翻瓣后，由前向后另翻腮腺筋膜瓣，切除腮腺后将筋膜瓣复位缝合，完整保留耳大神经和腮腺筋膜。结果：耳大神经在下颌角水平之上0-2cm依次分耳后、耳垂、耳前支，神经主干末段和分支起始段均分布于腮腺筋膜浅层表面，后者致密，其致密纤维包裹在神经周围。改良手术后2例（14．3％）发生轻度Frey’s综合征，无1例发生术区皮肤长期麻木、长期面瘫、涎瘘及肿瘤复发。结论：耳大神经各分支和腮腺筋膜具有不可代替的解剖生理功能，改良术式能将两者完好保留，显著降低术后并发症。     

30 and 32 kDa outer membrane proteins of Bordetella pertussis as a modulator on promoting degranulation of mast cells

Asthma is a common allergic disease of respiratorytract in human,especiallyin children [1-3] ,andcan be classified into 3 clinical types ,immediateasthmatic reaction (IAR) ,late asthmatic reaction(LAR) and biphasic asthmatic reaction(BAR)[4] ,in which approximately 60 %of cases intheworld belong to IgE-mediated IAR[5-7] . By theprevious epidemiological studies ,it was reportedthat respiratory infections by some pathogenic mi-croorganisms, such asBordetella pertussis, in-fluenza virus A o…     

Acceptance and utilization of HIV testing among the youth: a cross-sectional study in Techiman,Ghana

BackgroundIn Ghana, efforts including ‘Know Your Status’ campaign have been made to increase awareness and improve the uptake of HIV screening.ObjectiveThis study examined the acceptance and utilization of the HIV/AIDS ‘Know Your Status’ campaign and determine dthe differences in HIV testing by demographic characteristics among the youth in Techiman, Ghana.MethodThis study was a cross-sectional study conducted among the youth aged 15–24 years. A structured questionnaire was used to collect data from 200 purposively selected respondents.ResultsThe mean age of the respondents was 19.6±2.72 years. There was a universal awareness (100%) of HIV/AIDs, and were knowledgeable about the mode of transmission, symptoms and the prevention of HIV. A high proportion of the respondents (n=161, 80.5%) had heard about the ‘Know Your Status’ (KYS) campaign. Less than half of respondents (n=91, 45.5%) had tested for HIV, and only 16.5% (n=15/91) of respondents tested through the KYS campaign. Testing for HIV was associated with age (p<0.001) and marital status (p<0.001).ConclusionThe youth should therefore be targeted in the awareness and the ‘Know Your Status campaigns’, and in an effortsto promote screening for HIV.     

蓖麻蚕蛹生物反应器生产重组人表皮生长因子的研究

重组人表皮生长因子(rhEGF)已经广泛应用于医疗和化妆品。以杆状病毒AnpeNPV为基因表达载体,昆虫为生物反应器而建立的蛋白表达系统已获得成功。本文以人表皮生长因子(hEGF)基因替代柞蚕AnpeNPV中的核多角体基因而获得的AnpehEGF病毒为载体、蓖麻蚕蛹为生物反应器表达的rhEGF蛋白质,采用PCR、West-ern blot和ELISA等实验方法对其进行检测,硫酸铵沉淀和Ni-NTA Agrose亲和层析法对其进行分离纯化。实验结果显示,无论是在基因或是蛋白水平上都可检测到rhEGF的表达。AnpehEGF感染蚕蛹后第6d开始检测到rhEGF的表达量快速上升,第12d达到高峰,第3、6、9、12d的表达量分别为19.77、24.90、618.59、1 952.46ng/g,而到了病毒感染后期(第15d)出现了蛋白降解现象。表达的rhEGF通过分离纯化获得了较纯的产品。结果表明蓖麻蚕蛹作为生物反应器生产外源蛋白rhEGF是可行的,说明利用AnpeNPV和蓖麻蚕蛹可开发更加低成本而又高效的rhEGF生产新途径。