亚低温对重型颅脑损伤患儿Ⅰ型及Ⅱ型辅助性T细胞平衡漂移的影响

大量基础和临床研究证明,亚低温疗法能显著改善颅脑损伤病人的预后,对于在小儿领域的应用以及对机体细胞免疫功能的影响方面,目前研究较少.本研究通过对采用亚低温治疗的脑损伤患儿伤后T辅助淋巴细胞亚群进行测定,旨在探讨亚低温对重型颅脑损伤患儿伤后细胞免疫功能的影响,为临床治疗应用提供理论及实践依据.     

Dynamic axial control over optically levitating particles in air with an electrically-tunable variable-focus lens

Efficient delivery of viruses, proteins and biological macromelecules into a micrometer-sized focal spot of an XFEL beam for coherent diffraction imaging inspired new development in touch-free particle injection methods in gaseous and vacuum environments. This paper lays out our ongoing effort in constructing an all-optical particle delivery approach that uses piconewton photophoretic and femtonewton light-pressure forces to control particle delivery into the XFEL beam. We combine a spatial light modulator (SLM) and an electrically tunable lens (ETL) to construct a variable-divergence vortex beam providing dynamic and stable positioning of levitated micrometer-size particles, under normal atmospheric pressure. A sensorless wavefront correction approach is used to reduce optical aberrations to generate a high quality vortex beam for particle manipulation. As a proof of concept, stable manipulation of optically-controlled axial motion of trapped particles is demonstrated with a response time of 100ms. In addition, modulation of trapping intensity provides a measure of the mass of a single, isolated particle. The driving signal of this oscillatory motion can potentially be phase-locked to an external timing signal enabling synchronization of particle delivery into the x-ray focus with XFEL pulse train.OCIS codes: (140.7010) Laser trapping, (350.4855) Optical tweezers or optical manipulation     

硬皮病的超声诊断—附12例观察

采用高频探头(7.5MHz)对12例限局性硬度病皮损超声观察并与正常皮肤比较,前者皮肤全层平均厚3.6±0.4mm,真皮层2.9±0.4mm;后者全层厚2.0±0.4mm,真皮层1.4±0.4mm。两组有显著性差异(P<0.001)。4例硬皮病皮损还进行了显微镜下测量并与超声测量结果比较。认为超声检查硬度病具有实用价值。     

聚维酮联合阿霉素对E-J膀胱癌细胞的影响

目的通过聚维酮（PVP）联合阿霉素（adriamycin，ADM）对E-J膀胱癌细胞的影响，为开辟膀胱肿瘤术后膀胱灌注的新方法奠定基础。方法E-J膀胱癌细胞体外培养，实验分3组：PVP组（1％PVP）、ADM组、PVP＋ADM组，药物与细胞培养12h后用生理盐水冲洗再分别培养24h，流式细胞仪检测细胞凋亡的百分数。结果3组细胞在与药物培养12h后细胞凋亡的百分数分别为2．3％、37．3％、56．7％。生理盐水冲洗再分别培养24h，3组细胞凋亡的百分数分别为3．0％、39．4％、76．4％。结论PVP在体外显著提高ADM抗E-J膀胱癌细胞效麻．其作用机殚可能在于PVP的粘附性。     

转化生长因子β1诱导骨髓间充质干细胞体外构建纤维蛋白胶软骨模块

目的:观察体外构建骨髓间充质干细胞、改良纤维蛋白胶及生长因子的组织工程软骨模块的可行性。方法:实验于2004-07/12在南方医科大学附属珠江医院中心实验室进行。选取新西兰兔10只,密度梯度法分离培养兔骨髓间充质干细胞,在培养系统中加入生长因子(含10μg/L转化生长因子β1,100mol/L地塞米松,50mg/L维生素C,以及1%ITS-A。培养第2,4,6,8天采用四甲基偶氮噻唑法在酶标仪上检测吸光度值表示细胞增殖。于培养7,14d行细胞爬片甲苯胺蓝染色及Ⅱ胶原免疫组化。诱导后骨髓间充质干细胞种植于改良纤维蛋白胶支架上,加入生长因子诱导培养,于培养7,14,21d行形态组织学及透射电镜检查。结果:实验兔10只均进入实验结果分析。①四甲基偶氮噻唑法测定培养第6和8天后,实验组骨髓间充质干细胞增殖的吸收度值高于对照组(实验组:0.4554±0.0206,0.5348±0.0169;对照组:0.4270±0.0255,0.5057±0.0368,P<0.05)。②细胞爬片甲苯胺蓝染色细胞周围有蓝色基质。Ⅱ型胶原免疫组化染色阳性。③诱导后骨髓间充质干细胞在改良纤维蛋白胶支架中培养,Masson染色可见胞浆及细胞周围有胶原的形成;Ⅱ型胶原免疫组化染色阳性。透射电镜可见细胞代谢旺盛。结论:骨髓间充质干细胞、改良纤维蛋白胶及生长因子的组织工程模块体外培养系统中,改良纤维蛋白胶支架相容性好,转化生长因子β等生长因子促进骨髓间充质干细胞在改良纤维蛋白胶支架增殖,诱导分化成软骨细胞表型。     

郭志强教授运用崇阳理论治疗反复胚胎移植着床失败的经验

本文介绍郭志强教授运用崇阳理论治疗反复胚胎移植着床失败的经验.郭老认为妇人体质"阴常不足,阳亦常虚",形成"阴阳相济,阴基阳主"的崇阳理论.认为反复胚胎移植着床失败的主要病机为肾阳不足,胞宫寒冷,不能摄精成孕,提出治疗应从阳气着手,并秉持着求子先调经的原则,在胚胎移植前采用中药序贯疗法调经促孕,胚胎移植后予两固汤补肾温阳,顾护阳气,促进着床.     

广东粤西地区汉族人群红细胞酸性磷酸酶-腺苷脱氨酶-腺苷酸激酶的遗传学特性

目的:应用红细胞酸性磷酸酶-腺苷脱氨酶-腺苷酸激酶同步电泳分型,从血型遗传学角度对粤西汉族血液中3种酶型的表型分布、基目频率进行调查。方法:实验于2005-03/11在湛江师范学院完成。①血样取自广东湛江地区无血缘关系的汉族健康成人120例,3代内无与外族通婚史。②120例汉族健康成人均取耳血一两滴,渗于无菌纱布上,血痕直径1cm,低温干燥保存,7～14d内进行血痕表型检测。③基因频率计算采用Hardy-Weinbery平衡计算法。种群中某基因频率(%)=种群中该基因总数/种群中该对等位基因总数×100%;种群中某基因型频率(%)=种群中该基因型个体数/该种群的个体数×100%。结果:实验血样取自广东湛江地区无血缘关系的汉族健康成人120例,全部进入结果分析。①粤西汉族酸性磷酸酶、腺苷脱氨酶、腺苷酸激酶3种酶型表现型的观察值与期望值基本吻合(P>0.05),检测的酶型中未发现稀有型、变异型,亦未发现罕见基因。②在粤西汉族群体中,酸性磷酸酶具有中高鉴别能力(积累能力值0.5043),腺苷脱氨酶为低鉴别能力(积累能力值0.2922),而腺苷酸激酶无鉴别能力(积累能力值0.0023)。结论:粤西汉族人群3种检测酶型中未发现稀有型、变异型,亦未发现罕见基因。符合Hardy-Weinbery遗传平衡定律。     

ATP binding cassette G8 T400K polymorphism may affect the risk of gallstone disease among Chinese males

BACKGROUND: Supersaturation of bile with cholesterol is a primary step in the formation of cholesterol gallstones. ATP binding cassette (ABC) G5 and G8 play an important role in regulating sterol absorption and secretion. To investigate a possible association between transporter gene polymorphism and gallstone formation, we examined five common polymorphisms in the ABCG5 (Q604E) and ABCG8 (D19H, Y54C, T400K, A632V) genes in patients with gallstone disease (GS). METHODS: Study subjects included 287 patients with GS and 219 gallstone free controls (GSF). Polymorphisms were determined using PCR-RFLP analysis or the Taqman MGB assay. Plasma and biliary lipid levels were measured. RESULTS: 2 SNPs of ABCG8 gene (Y54C and T400K) showed strong linkage disequilibrium (D'=0.824, r2=0.579). Male carriers of the less frequent K allele of ABCG8 T400K had a 2.31-fold elevated risk [95% confidence interval (CI) 1.12 approximately 4.76, P=0.023] for gallstone disease compared to male with the common genotype after the adjustment for age, body mass index. Males with the K allele had lower plasma triglyceride (P=0.044) and biliary phospholipid (P=0.035) levels than TT homozygotes. No such association was found in female or other 4 SNPs. CONCLUSIONS: These findings indicate that the T400K polymorphism in ABCG8 may be associated with the incidence of gallstone disease in males.     

Computer-Delivered Social Norm Message Increases Pain Tolerance

Background

Few experimental studies have been conducted on social determinants of pain tolerance.

Purpose

This study tests a brief, computer-delivered social norm message for increasing pain tolerance.

Methods

Healthy young adults (N?=?260; 44 % Caucasian; 27 % Hispanic) were randomly assigned into a 2 (social norm)?×?2 (challenge) cold pressor study, stratified by gender. They received standard instructions or standard instructions plus a message that contained artifically elevated information about typical performance of others.

Results

Those receiving a social norm message displayed significantly higher pain tolerance, F(1, 255)?=?26.95, p?<?.001, η _p ² ?=?.10 and pain threshold F(1, 244)?=?9.81, p?=?.002, η _p ² ?=?.04, but comparable pain intensity, p?>?.05. There were no interactions between condition and gender on any outcome variables, p?>?.05.

Conclusions

Social norms can significantly increase pain tolerance, even with a brief verbal message delivered by a video.