姜黄素对口腔舌癌细胞增殖与转移能力影响的实验研究

目的 探讨姜黄素对口腔舌癌细胞增殖与转移能力的作用及其作用机制.方法分别以0、5、10、20、30、60、100μmol·L-1姜黄素处理人舌癌细胞SCC-4 24 h,采用MTT法、侵袭实验、流式细胞仪、免疫荧光显微镜分别检测姜黄素对口腔舌癌细胞增殖与转移能力的影响.然后,采用cDNA微陈列芯片技术筛选和实时定鼍RT...     

减阻牵张正畸牙移动过程中牙周组织超微结构变化的研究

目的：研究减阻牵张正畸牙移动过程中牙周组织超微结构的变化，为此方法矫治正畸牙提供理论依据。方法：将6只杂种犬随机分为2周、3周、6周二组，拔除下颌两侧第二前磨牙，随机选择一侧为实验侧，实施减阻牵张措施加快牙齿移动速度，另一侧为空白对照侧，实验侧加力2周、固定保持1周、4周时，制备移动牙标本，进行透射电镜观察。结果：对照侧无明显成骨及破骨迹象；加力2周组张力侧成骨细胞、成纤维细胞的胞浆内高尔基体、内质网等细胞器非常丰富，分泌功能旺盛；压力侧破骨细胞增多，高尔基体发达，线粒体、溶酶体增多，成纤维细胞细胞器发达，间充质细胞、活跃的巨噬细胞增多；随着固定保持时间的延长，实验侧与对照侧超微结构的差异变小。结论：正畸牙压力侧减阻后，在强、高频率加力方式作片用下，牙周组织细胞功能旺盛、改建活跃，无不可逆的细胞变性坏死变化。     

几种消毒剂对藻酸盐印模材料的消毒效果

作者为了选择对藻酸盐印模材料有效的消毒剂和消毒方法，采用２％碱性戊二醛、１％过氧乙酸、２％甲醛对３０名受试者的藻酸盐印模进行消毒，并在消毒前后进行细菌培养及鉴定。结果发现：消毒前的印模有９种细菌被检出，污染率高达９６％；消毒后的印模细菌检出率为零。作者认为，本研究所用消毒剂、消毒方法效果良好，值得沿用。     

遗传性神经系统离子通道病基因治疗的研究进展

目的遗传性神经系统离子通道病现有的治疗方法往往难以控制病情或者患者耐受度较低,因此探索新的治疗方法势在必行。基因治疗在多项研究中显示可能应用于神经系统疾病,并可能成为未来遗传性神经系统离子通道病的有效治疗手段。同时,光遗传学、化学遗传学方法、基因编辑技术的不断进步也为遗传性神经系统离子通道病的基因治疗提供了良好的发展基础。我们将就遗传性神经系统离子通道病基因治疗的方法机制、转导载体、启动子及给药途径作一综述。     

Microsatellite markers development in the clam,Paphia amabilis (Philippi, 1847)

The clam Paphia amabilis is an important edible shellfish in southern China and Japan. Recently the natural populations of this species has significantly decreased due to the environmental changes and overfishing. To support the conservation and aquaculture of this species, we developed 12 microsatellite markers for the clam and characterized them in a wild population. These markers were polymorphic with the number of alleles per locus ranged from 2 to 9, the observed heterozygosity and expected heterozygosity ranged from 0.22 to 0.79 and from 0.33 to 0.87, respectively. Ten of the 12 markers conformed to Hardy–Weinberg equilibrium. This is the first set of microsatellites developed for P. amabilis and these markers will promote the genetic studies and species protection of this species.     

The early and mid-term results of mitral valve repair for mitral regurgitation in children

Purpose

To review the surgical techniques and mid-term results of mitral valve repair in children with moderate or severe mitral regurgitation (MR).

Methods

One hundred and seven children with moderate or severe MR, aged 19.6 ± 8.5 months, were enrolled in this study. The surgical techniques used for mitral valve repair varied according to the mitral valve morphology, and included annuloplasty, annuloplasty ring, cleft closure, reconstruction of the posterior leaflet, etc. The concomitant cardiac anomalies were treated simultaneously. The results of repair were evaluated by transesophageal echocardiography performed during the operation and by serial transthoracic echocardiography performed during the follow-up.

Results

One hundred and six cases had no more than mild regurgitation intraoperatively, whereas only one case had moderate regurgitation. This patient underwent redo repair immediately, and the subsequent regurgitation was trivial. The in-hospital mortality rate was 0.9 % (1/107). The average follow-up was 46.5 ± 8.2 months. One patient died of heart failure 10 months postoperatively. The freedom from moderate or severe regurgitation after mitral valve repair was 92.3 ± 3.3 %.

Conclusion

Pediatric patients with moderate or severe MR require early surgical treatment. The early and mid-term results of mitral valve repair in pediatric patients were satisfactory.     

Disposable circumcision suture device： clinical effect and patient satisfaction

In our experience patients undergoing circumcision are mostly concerned about pain and penile appearances. We conducted a prospective randomized trial to assess the benefits of a new disposable circumcision suture device （DCSD）. A total of 942 patients were equally divided into three groups （conventional circumcision, Shang ring and disposable suture device group）. Patients in the DCSD group were anesthetized with compound 5% lidocaine cream, the others with a 2% lidocaine penile block. Operation time, intra-operative blood loss, incision healing time, intra-operative and post-operative pain, the penile appearance and overall satisfaction degree were measured. Operation time and intra-operative blood loss were significantly lower in the Shang ring and suture device groups compared to the conventional group （P 〈 0.001）. Intra-operative pain was less in the suture device group compared With the other two groups （P 〈 0.001）; whereas post-operative pain was higher in the conventional group compared to the other two groups （P 〈 0.001）. Patients in the suture device （80.57%） and Shang ring （73,57%） groups were more satisfied with penile appearances compared with the conventional circumcision group （20.06%, P 〈 0.05）. Patients in suture device group also healed markedly faster than the conventional group （P 〈 0.01）. The overall satisfaction rate was better in the suture device group （78.66%） compared with the conventional （47.13%） and Shang ring （50.00%） groups （P 〈 0.05）. The combination of DCSD and lidocaine cream resulted in shorter operation and incision healing times, reduced intra-operative and post-operative pain and improved patient satisfaction with the cosmetic appearances.     

人工肛门袋的改良研究与肠造口护理近况

对肛门袋的种类、不同类型肛门袋的使用特点及肠造口皮肤的护理问题进行了综述。     

中药止痛膏在癌性疼痛控制中的应用研究

[目的]研究中药止痛膏在癌性疼痛控制中的疗效。[方法]住院肿瘤病人71例,随机分为两组。实验组36例,采用中药止痛膏外敷止痛;对照组35例,口服奥施康定止痛。观察两组病人镇痛前后镇痛评分、血压、脉搏变化,以及使用奥施康定情况及使用中药止痛膏病人的主观感受。[结果]两组病人镇痛前后镇痛评分、血压、脉搏变化比较差异无统计学意义,实验组病人使用镇痛药物剂量明显减少,主观感觉较好。[结论]中药止痛膏外敷能有效缓解癌性疼痛,减少镇痛药物的使用。