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排序方式: 共有629条查询结果,搜索用时 140 毫秒
71.
Julien Schaller Hlne Maby-El Hajjami Sylvie Rusakiewicz Kalliopi Ioannidou Nathalie Piazzon Alexandra Miles Dla Golshayan Olivier Gaide Daniel Hohl Daniel E. Speiser Karin Schaeuble 《Oncotarget》2021,12(7):638
Although tumor-associated lymphangiogenesis correlates with metastasis and poor prognosis in several cancers, it also supports T cell infiltration into the tumor and predicts favorable outcome to immunotherapy. The role of lymphatic vessels in skin squamous-cell carcinoma (sSCC), the second most common form of skin cancer, remains mostly unknown. Although anti-PD-1 therapy is beneficial for some patients with advanced sSCC, a greater understanding of disease mechanisms is still needed to develop better therapies.Using quantitative multiplex immunohistochemistry, we analyzed sSCC sections from 36 patients. CD8+ T cell infiltration showed great differences between patients, whereby these cells were mainly excluded from the tumor mass. Similar to our data in melanoma, sSCC with high density of lymphatic endothelial cells showed increased CD8+ T cell density in tumor areas. An entirely new observation is that sSCC with perineural infiltration but without metastasis was characterized by low lymphatic endothelial cell density. Since both, metastasis and perineural infiltration are known to affect tumor progression and patients’ prognosis, it is important to identify the molecular drivers, opening future options for therapeutic targeting. Our data suggest that the mechanisms underlying perineural infiltration may be linked with the biology of lymphatic vessels and thus stroma. 相似文献
72.
Estibaliz Gamboa Moreno Maider Mateo-Abad Lourdes Ochoa de Retana García Kalliopi Vrotsou Emma del Campo Pena Álvaro Sánchez Perez Catalina Martínez Carazo Juan Carlos Arbonies Ortiz M. Ángeles Rúa Portu Koldo Piñera Elorriaga Amaya Zenarutzabeitia Pikatza Miren Nekane Urquiza Bengoa Tomás Méndez Sanpedro Ana Oses Portu Miren Bakarne Aguirre Sorondo Rafael Rotaeche del Campo 《Primary Care Diabetes》2019,13(2):122-133
Aim
The purpose of this study was to assess the efficacy of the Spanish Diabetes Self-Management Program (SDSMP) versus usual care in adults with type 2 diabetes mellitus (T2DM) residing in a Spanish region.Methods
A two-year follow-up randomised controlled trial. The intervention consisted of 6 weekly structured peer-to-peer workshops. The primary outcome was change in HbA1c levels. Secondary outcomes included other clinical measures, quality of life, self-efficacy, life-style changes, medication and use of healthcare services. Mixed effect models were fitted.Results
n=297 patients were recruited in each study arm. Baseline HbA1c levels were comparable in both groups with an overall mean 7.1 (SD=1.2). The intervention did not significantly modify HbA1c, or other cardiovascular variables. Significant improvements were seen in self-efficacy, and in particularly its disease control component. Certain differences were also observed in the use of healthcare resources and medication consumption. High workshop participation and satisfaction rates were achieved.Conclusion
HbA1c reductions are difficult to obtain in adequately controlled patients. On the other hand, raising awareness on one’s disease can increase disease control self-efficacy. This finding, accompanied by reduced medication consumption and healthcare use rates, highlights that usual care would be benefited by incorporating certain SDSMP aspects.Trial registration: ClinicalTrials.gov identifier NCT01642394 相似文献73.
Nikolaos K. Gatselis Aigli G. Vakrakou Kalliopi Zachou Theodoros Androutsakos Kalliopi Azariadis Gregorios Hatzis 《Autoimmunity》2017,50(2):125-132
Deoxyribonuclease1 (DNase1) is involved in chromatin degradation of apoptotic cells. Its deficiency results in accumulation of self-DNA, which in turn may induce inflammation and autoimmunity. We assessed for the first time serum DNase1-activity in a large consecutive cohort of treatment-naïve patients with autoimmune liver diseases (ALD). DNase1-activity was determined by single radial enzyme-diffusion (SRED) at diagnosis of 224 patients with autoimmune hepatitis (AIH), 249 with primary biliary cirrhosis (PBC) and 36 with primary sclerosing cholangitis (PSC). Sera from 146 patients with chronic hepatitis B or C, 140 with nonalcoholic fatty liver disease/nonalcoholic steatohepatitis (NAFLD/NASH) and 114 healthy individuals served as disease and healthy controls. Available serum samples during remission from 50 AIH and 39 PBC patients were also investigated by paired analyzes. DNase1-activity was significantly lower in AIH, PBC and PSC compared to viral hepatitis (p?0.02, p?0.001, p?=?0.03), NAFLD/NASH (p?0.001) and healthy (p?0.001). No significant difference was found in between each specific ALD. In AIH, DNAse1-activity was positively correlated with aspartate aminotransferase (AST) (p?0.02), bilirubin (p?0.01) and increased IgG (>1400?mg/dl; p?0.05); in PBC, with AST (p?0.01), alanine aminotransferase (ALT) (p?0.03) and anti-mitochondrial antibodies (AMA) (p?=?0.008). In PSC, DNase1-activity was inversely associated with alkaline phosphatase (ALP) (p?0.05). In AIH, complete responders were characterized by increased baseline DNase1-activity compared to partial responders, relapsers and non-responders (p?0.02), whereas it was significantly increased after achievement of remission (p?0.001). Serum DNase1-activity is significantly decreased in ALD patients, indicating its potential implication in their pathogenesis. Furthermore, DNase1-activity could be used as a new surrogate biomarker for predicting response to AIH treatment. 相似文献
74.
75.
Acute combined effects of olive oil and wine on pressure wave reflections: another beneficial influence of the Mediterranean diet antioxidants? 总被引:1,自引:0,他引:1
Papamichael CM Karatzi KN Papaioannou TG Karatzis EN Katsichti P Sideris V Zakopoulos N Zampelas A Lekakis JP 《Journal of hypertension》2008,26(2):223-229
OBJECTIVES: Combined consumption of olive oil and wine is common in the Mediterranean diet, but there are no data concerning their synergistic haemodynamic response. We sought to determine the combined postprandial effects of wine and olive oil on wave reflections and central haemodynamics. METHODS: Fifteen healthy subjects consumed four standard meals on different days, containing 50 g of olive oil and 250 ml of wine, in a randomized cross-over study design. Two types of wine [red (R) and white (W)] and two types of olive oil [green (G) and refined (O) (rich and poor in antioxidants, respectively)] were used in all possible combinations (RO, RG, WO and WG). Applanation tonometry and aortic pulse wave analysis were performed when fasting and 1, 2 and 3 h postprandially. A second group of 15 healthy individuals matched for age, gender and body mass index served as the control group. RESULTS: All meals decreased AIx (RO and RG, P < 0.001; WO, P = 0.007; and WG, P = 0.039). The AIx reduction after RG, RO, WO and WG was significantly different from the respective AIx response of the control group. No difference was observed in the reduction of AIx between sessions, but a significantly earlier peak decrease in AIx, as well as a more prolonged decreasing effect, was observed after RG and RO consumption compared to WO and WG. Central systolic and diastolic pressures were diminished after all four combinations of wine and olive oil (P < 0.05). CONCLUSIONS: Combined consumption of wine and olive oil provided beneficial postprandial effects on haemodynamics. These findings reveal an additional favourable effect of components of the Mediterranean diet on haemodynamics in the postprandial state. 相似文献
76.
Ductal plate malformation and congenital hepatic fibrosis Clinical and histological findings in four patients. 总被引:1,自引:0,他引:1
Olga Giouleme Nikolaos Nikolaidis Konstantinos Tziomalos Kalliopi Patsiaoura Themistoklis Vassiliadis Nikolaos Grammatikos Vassilios Papanikolaou Nikolaos Eugenidis 《Hepatology research》2006,35(2):147-150
Congenital hepatic fibrosis belongs to the fibrocystic diseases of the liver and represents ductal plate malformation of interlobular bile ducts, along with a destructive cholangiopathy associated with fibrosis. Four patients with congenital hepatic fibrosis are described. Their median age at presentation was 25 years; none of them had a family history of liver or renal disease. Variceal bleeding was the initial manifestation in three patients. All of them required frequent endoscopic variceal ligation sessions and distal splenorenal shunting was also performed in two, almost obviating the need for further variceal ligation. Variceal bleeding did not recur during follow-up. One of these three patients rarely exhibited acute cholangitis; administration of ursodeoxycholic acid resulted in complete remission. In contrast, the fourth patient showed frequent severe episodes of acute cholangitis but normal cholangiographic findings. He underwent liver transplantation but died 2 months later. Laboratory findings disclosed pancytopenia in all patients whereas hepatic synthetic capacity was well preserved. Renal function was unaffected despite the presence of polycystic kidneys in two patients. In summary, congenital hepatic fibrosis can also be diagnosed in older ages, might have strikingly different manifestations and is associated with prominent portal hypertension necessitating aggressive management in order to prevent variceal bleeding. 相似文献
77.
Kanellos D Pramateftakis MG Demetriades H Zacharakis E Angelopoulos S Mantzoros I Kanellos I Despoudi K Zaraboukas T Koliakos G Galovatsea K Lazaridis H 《International journal of colorectal disease》2008,23(12):1185-1191
Aim The aim of this experimental study was to investigate the effect of intraperitoneal administration of oxaliplatin on the healing
of colonic anastomoses when injected immediately after colon resection.
Materials and methods Thirty male Wistar rats were used. During the operation, the rats were randomized to two groups of 15 rats each. Immediately
after colonic anastomoses were performed, the rats were injected intraperitoneally with either 3 ml of 0.9% NaCl solution
or oxaliplatin (2.4 mg/kg body weight) depending on their group. All rats were killed on the eighth postoperative day. The
anastomoses were examined macroscopically. The anastomotic bursting pressures were recorded, the anastomoses graded histologically,
and the hydroxyproline tissue contents determined.
Results Anastomotic leakage was noted in four rats (26.7%) of the oxaliplatin group, whereas no anastomotic dehiscence was detected
among rats of the control group (p = 0.016). The adhesion formation at the anastomotic sites and the inflammatory cell infiltration were significantly higher
in the oxaliplatin group than in the control group (p = 0.001). The bursting pressures (p = 0.001), the hydroxyproline tissue content (p = 0.001), the neoangiogenesis (p = 0.033), the fibroblast activity (p = 0.001), and the collagen deposition (p = 0.001) were significantly lower in the oxaliplatin group in comparison to the control group.
Conclusion The immediate postoperative intraperitoneal administration of oxaliplatin seems to impair healing of colonic anastomoses in
rats. 相似文献
78.
Evangelos J Giamarellos-Bourboulis Efterpi Apostolidou Malvina Lada Ioannis Perdios Nikolaos K Gatselis Iraklis Tsangaris Marianna Georgitsi Magdalini Bristianou Theodora Kanni Kalliopi Sereti Miltiades A Kyprianou Anastasia Kotanidou Apostolos Armaganidis 《Critical care (London, England)》2013,17(5):R247
Introduction
The aim of this study was to investigate the kinetics of immunoglobulin M (IgM) during the different stages of sepsis.Methods
In this prospective multicenter study, blood sampling for IgM measurement was done within the first 24 hours from diagnosis in 332 critically ill patients; in 83 patients this was repeated upon progression to more severe stages. Among these 83 patients, 30 patients with severe sepsis progressed into shock and IgM was monitored daily for seven consecutive days. Peripheral blood mononuclear cells (PBMCs) were isolated from 55 patients and stimulated for IgM production.Results
Serum IgM was decreased in septic shock compared to patients with systemic inflammatory response syndrome (SIRS) and patients with severe sepsis. Paired comparisons at distinct time points of the sepsis course showed that IgM was decreased only when patients deteriorated from severe sepsis to septic shock. Serial measurements in these patients, beginning from the early start of vasopressors, showed that the distribution of IgM over time was significantly greater for survivors than for non-survivors. Production of IgM by PBMCs was significantly lower at all stages of sepsis compared with healthy controls.Conclusions
Specific changes of circulating IgM occur when patients with severe sepsis progress into septic shock. The distribution of IgM is lower among non-survivors. 相似文献79.
80.
Kontakis GM Papadokostakis GM Alpantaki K Chlouverakis G Hadjipavlou AG Giannoudis PV 《Injury》2006,37(10):953-960
PubMed and Ovid Medline were searched to investigate the role of intramedullary nailing in the treatment of diaphyseal humeral non-union. Of 474 abstracts, 9 retrieved articles fulfilled our inclusion criteria and described management in 166 cases of humeral shaft non-union using several types of intramedullary nails. Union ranged from 40 to 100%; part of the variation could be explained by the quality index of the selected articles. Exchange nailing alone was not effective. Exposure of the fracture site and use of bone grafting were key to successful treatment. The total incidence of severe complications, including radial nerve damage, was very low. Thus, intramedullary nailing in the treatment of humeral shaft non-unions can lead to successful outcomes when associated with autologous bone grafting. 相似文献