中药降低血清白介素6水平的临床应用分析

目的探讨用于降低血清白介素6(IL-6)水平的常用中医药物。 方法用计算机以"IL-6"和"中药"为检索主题词,以2007年至2018年为检索时间范围,分别在中国知网、维普、万方期刊服务平台检索期刊文献,从检索出的期刊文献中筛选出符合标准的文献,并从中筛选出符合纳入标准的中医药物进行分析。 结果共检索出1 067篇期刊文献(其中中国知网183篇,维普351篇,万方533篇),485篇符合纳入标准,包括160余味中药,最终纳入18味中医药物。其中清热药7味、补虚药6味、活血化瘀药2味、利水渗湿药2味、解表药2味、泻下药1味、收涩药1味。清热药中5/7归属肝经,补虚药中5/6归属脾经,出现频率最高的药物为黄芪。 结论清热药和补虚药是用于降低血清IL-6水平的常用中医药物,泄肝与补脾是用于降低血清IL-6水平的常用中医治疗方法。     

急性白血病P_(170)和CD_(34)抗原共同表达及其临床意义

目的 :探讨急性白血病 (AL ) P1 70 和 CD34 抗原共同表达与患者化疗疗效的关系。方法 :对 81例 AL患者的单个核细胞分别用抗 P1 70 单克隆抗体 UIC2 和抗 CD34 单克隆抗体标记后 ,经流式细胞仪检测后分成 4组 ,观察其与 AL 患者临床疗效的关系。结果 :P1 70 和 CD34 抗原共同高表达组的 AL 患者的临床疗效最差 ;P1 70 和 CD34 比较 ,P1 70 表达增高预后更差。结论 :P1 70 和 CD34 同时高表达临床缓解率更低 ,更有预后意义     

Symbiotic composite composed of MoS2 and pelagic clay with enhanced disinfection efficiency

Molybdenum disulfide (MoS₂) has attracted increasing attention as a promising photocatalyst. In addition to its application in photocatalytic hydrogen production and pollutant degradation, MoS₂ is also used in water disinfection. However, its poor disinfection performance limits its practical utility. Herein, we prepared a symbiotic composite composed of MoS₂ and pelagic clay (MoS₂/PC) as a photocatalyst for water disinfection. The composite achieved a high disinfection rate of 99.95% to Escherichia coli (E. coli) under visible light illumination, which is significantly higher than that of bulk MoS₂ (61.87%). Characterization shows that abundant hydroxyl groups in illite/montmorillonite (I/M) formed during hydrothermal synthesis of MoS₂, which contributed to the enhanced disinfection activity. Those hydroxyl groups can attract photogenerated holes through electrostatic attraction, and facilitate the separation of photogenerated charge carriers, thereby enhancing the disinfection activity. Moreover, the good hydrophilicity of pelagic clay improves the dispersity of MoS₂ in water, which is beneficial for its utility in aqueous solutions. In addition, the symbiotic structure restricts the growth and aggregation of MoS₂ nanosheets and shortens the diffusion distance of charge carriers to the material surface, further reducing the recombination of electrons and holes. This study provides a way to improve the disinfection activity of MoS₂ and also sheds light on high value-added utilization of pelagic clay.

A symbiotic composite MoS₂/PC was prepared and showed an enhanced disinfection efficiency to E. coli 8739 under visible light.     

HPLC测定中药多成分总量溶度参数的理论及实验研究

目的:创立HPLC指纹图谱总量几何平均保留时间测定中药多成分表观溶度参数的理论与方法。方法:运用指纹图谱总量几何平均保留时间能表达HPLC指纹图谱特征峰的整体趋势特点,建立其与多成分表观溶度参数的内在函数式,用已知溶度参数的芦荟大黄素类成分进行实验证明,并测定左金方的表观溶度参数。HPLC条件:A lltech Apollo C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱;检测波长430 nm;柱温30℃。结果:建立了指纹图谱几何平均保留时间与总量表观溶度参数关联的数学表达式,用此法测得芦荟大黄素等混合物表观溶度参数为36.12 J1/2.cm-3/2,按峰面积比算得值为35.57 J1/2.cm-3/2,按摩尔分数算得值36.07 J1/2.cm-3/2。结论:HPLC指纹图谱总量几何平均保留时间法能同时测定多个成分及总量的表观溶度参数,这对研究中药的配伍溶解规律,单味中药剂改工艺研究奠定理论基础。     

乳癌患者99m Tc-MIBI显像及癌组织中P-糖蛋白、多药耐药相关蛋白测定

目的:评价乳癌患者99mTc-甲氧基异丁基异腈(MIBI)显像结果,并检测癌组织中P-糖蛋白(P-gp)、多药耐药相关蛋白(MRP)的表达.方法:42例乳腺癌患者静脉注射99mTc-MIBI 555～740 MBq(15～20 mCi),10 min及3 h后显像.计算早、晚期肿瘤部位平均放射性计数与对侧相应部位平均放射性计数比值(T/N)、清除率.术后标本采用免疫组织化学法检测P-gp、MRP的表达.结果:根据P-gp、MRP免疫组织化学结果将42例患者分为4组:A组P-gp阳性、MRP阳性,7例;B组P-gp阳性、MRP阴性,15例;C组P-gp阴性、MRP阳性,9例;D组P-gp阴性、MRP阴性,11例.早期T/N值,A组、B组与D组比较,差异均有统计学意义(P均＜0.05),其余各组间差异无统计学意义(P均＞0.05).晚期T/N值,各组间差异无统计学意义(F=0.460,P=0.650).清除率,A组与B组、C组、D组之间,B组、C组与D组之间,差异均有统计学意义(P均＜0.05),而B组与C组之间,差异无统计学意义(P＞0.05).结论:乳癌99mTc-MIBI显像计算清除率可用于评估癌组织中P-gp、MRP的表达.     

Rheological Properties and Early-Age Microstructure of Cement Pastes with Limestone Powder,Redispersible Polymer Powder and Cellulose Ether

In order to study the synergistic effects of organic and inorganic thickening agents on the rheological properties of cement paste, the rheological parameters, thixotropy cement-paste containing limestone powder (LP), re-dispersible polymer powder (RPP), and hydroxypropyl methylcellulose ether (HPMC) were investigated using the Anton Paar MCR 102 rheometer at different resting times. The early-age hydration process, hydration products, and microstructure were also analyzed with scanning electron microscopy (SEM) and thermogravimetry analyses (TGA). The results showed that the addition of LP, RPP, and HPMC affected the rheological properties of cement paste, but the thickening mechanism between organic and inorganic thickening agents was different. The small amount of LP increased the plastic viscosity but decreased the yield stress of cement paste due to its dense filling effect. Adding 1% of RPP improved the thixotropic property of cement paste by 50%; prolonging the standing time could improve the thixotropic performance by as much as two times. Only 0.035% HPMC added to the cement paste increased the plastic viscosity by 20%, while the yield stress increased nearly twice. The more HPMC added, the more significant effect it showed. Cement paste compounds with LP, RPP, and HPMC balanced the yield stress and plastic viscosity and improved the thixotropy. The C-L6-R1.0-H0.035 paste presented as a pseudoplastic, its rheological indexes were close to one, and it was hardly affected by the resting time. The composite superposition effect of organic and inorganic thickening agents reduced the impact of resting time for all pastes. As the organic thickening component inhibited the hydration more than the LP promoted the hydration of the cement paste, indicating that the C-L6-R1.0-H0.035 paste remained in the particle fusion stage after curing for three days, as shown by the SEM images.     

芦荟大黄素诱导肿瘤细胞凋亡的研究进展

芦荟大黄素是蓼科植物掌叶大黄的根和块茎中含有的一种活性成分。研究发现其对多种肿瘤细胞株具有抑制作用,文章综述了近年来有关芦荟大黄素诱导肿瘤细胞株凋亡的作用及其机理的研究进展。     

王真教授治疗肺癌术后咳嗽的经验

[目的]总结王真教授治疗肺癌术后咳嗽的经验。[方法]跟随王真教授临诊,收集肺癌术后咳嗽的病案,并查阅肺癌术后咳嗽的相关文献,结合当前西医学对于肺癌术后咳嗽机制的研究,总结王真教授关于肺癌术后咳嗽中医病因病机的认识,以及临床常见的证型和对应的治疗方药,并举验案一则加以佐证。[结果]现代医学认为,肺癌术后咳嗽可能与纵隔淋巴结清扫、肺C纤维受损、解剖结构改变、胃食管反流、瞬时受体电位香草酸亚型1(transient receptor potential vanilloid 1,TRPV1)表达增加、气管插管等有关。王真教授认为肺癌术后咳嗽患者总的病机特点属本虚标实,本为气血不足、肺阴亏虚,标为风邪、痰邪、气逆、气滞等。常见证型有风咳型、胃气上逆型、痰热郁肺型、咽喉不利型、肝郁气滞型等,临床上常自拟经验方或化裁经典验方如旋覆代赭汤、半夏厚朴汤、柴胡疏肝散等,并常于药方中酌加补益气血、滋阴润肺之品,止咳不忘补虚。所举验案属胃气上逆型咳嗽,治以旋覆代赭汤加减,和胃降逆,清肺化痰止咳,取得较好疗效。[结论]王真教授在治疗肺癌术后咳嗽上注重病证统筹,分型施治,临床疗效显著,值得传承推广。     

基于信息效能模型定性访谈的样本量确定

在全科医学领域应用定性研究,可以更好地体现以患者为中心的医疗模式,促进基层医疗卫生服务发展,具有重要的实践意义。目前国外在全科医学领域应用定性研究已经比较成熟,而国内尚处于早期发展阶段。因此,在开展定性研究的过程中会碰到很多实际的操作问题,其中一个常见问题就是样本量的问题。但是,对于定性研究的样本量,没有人能给出一个标准答案。总体来说,一项研究的深度和广度是相互制约的。由于定性研究注重对研究现象进行比较深入、细致的理解,通常认为定性研究中资料的丰富程度远比样本的数量重要,但是在撰写定性研究计划时,样本量仍然需要提前考虑。定性研究的样本量需要考量研究所涉及的与样本量有关的维度。本文介绍基于信息效能模型考虑定性访谈的样本量,为定性研究者初步拟定访谈对象的数量提供参考依据。     

某医院新冠肺炎抗疫应急药房运行特点分析

目的：某医院逐渐总结出了针对突发疫情的应急药房运行模式。旨在分析该模式相比于传统模式的优势,为突发性公共卫生事件中建立应急药房体系提供参考。方法：收集某新冠肺炎专科医院应急药房工作运行数据,并进行分析,总结出应急药房的特点和建议。结果：通过对该应急药房的运行模式进行考察,证明该药房是可以胜任新冠肺炎救治药品保障工作的,能为抗击新冠肺炎疫情起到积极的作用。结论：该药房的运行对于新冠肺炎疫情是一种较优良的应急药房运行模式。