FS04.1Formaldehyde allergy – clinically relevant threshold reactions

The objective of the study was to establish eliciting threshold concentrations of diazolidinyl urea (Germall II)– derived formaldehyde in formaldehyde and/or in diazolidinyl urea – sensitive patients, using a leave‐on face cream formulation in a repeated open application test (ROAT) applied to different anatomical regions.
150 patients with known formaldehyde allergy were reviewed for inclusion in the study. 108 patients were contacted and in 65 patients the formaldehyde sensitisation was reconfirmed by a patch test. Four groups of 10 formaldehyde allergic subjects were exposed to 0.05%, 0.15%, 0.3% and 0.6% diazolidinyl urea, corresponding to approximately 50, 100, 200 and 400 ppm free formaldehyde, respectively.
Additional 10 individuals allergic to the formaldehyde donor – diazolidinyl urea itself – were exposed to 0.15% diazolidinylurea, corresponding to approximately 100 ppm free formaldehyde and 10 healthy non‐allergic individuals were exposed to 0.6% of diazolidinylurea (approximately 400 ppm free formaldehyde).
A ROAT was performed in a scheduled sequence: upper arm, neck and face.
Contact allergy reactions were elicited in 39 out of 58 formaldehyde‐sensitive and in 5 out of 7 diazolidinyl urea‐sensitive individuals.
Elicitation responses were dose‐ and anatomical region – dependent.
No reactions were observed at the lowest dose, suggesting that an elicitation threshold was attained in the study.     

P34Reproducibility of the positive patch test reactions with the TRUE Test

The patch test results of patients tested at least twice at our clinic during a period of 11 years were analyzed with regard to reproducibility of the positive patch test reactions found in the first patch testing session.
A retrospective analysis of 257 patients who have been tested with the TRUE Test at least twice between 1991 and 2002 and had a minimum of one positive reaction in the first testing session was done. Test reactions were read routinely after 3 and 5–7 days.
In the first testing session a total of 299 positive reactions were found. Of these allergens, 151 (51%) stayed positive in the second test, 31 (10%) produced a doubtful reaction, 67 (22%) a negative response, and 50 (17%) were not retested because of strong positive reactions in the first test. Of the individual allergens, positive reactions from thiuram‐mix, kathon CG and colophony were the most reproducible.
If it is assumed that allergens which were not retested because of strong positive reactions would have shown positive reactions in a retest, a total of 68% of positive reactions were reproduced. A multitude of factors, such as: avoidance of relevant allergens between tests, diminished sensitivity, retesting within a period of more or less active dermatitis, different observers, methodological error, and prior false‐positive responses, influence the reproducibility of patch tests. The results will be put in perspective.     

白及多糖对酪氨酸酶活性及黑色素生成的影响

目的:观察白及多糖对酪氨酸酶(TYR)活性及黑色素形成相关蛋白TYR、小眼畸形相关转录因子(MITF)表达的影响。方法:超声提取白及多糖粗品。用不同浓度的白及多糖(0.05、0.1、0.2、0.4、0.8 g/L)和曲酸(阳性对照,0.1、0.2、0.4 g/L)作用于小鼠B16黑色素瘤细胞,用于检测细胞活性、TYR活性;各组细胞分别加入α-黑色素细胞刺激素(α-MSH)及不同浓度药物共培养后检测黑色素含量;将细胞分为空白组、模型组(α-MSH)、曲酸组及白及多糖低、中、高剂量组,予相应干预48 h后,应用Western blot检测B16细胞TYR和小眼畸形相关转录因子(MITF)的蛋白表达情况。结果:不同浓度曲酸组、白及多糖(0.05~0.8 g/L)组、空白组间的细胞活性差异无统计学意义(P0.05)。与空白组比较,白及多糖在浓度0.1~0.8 g/L对TYR的活性有显著抑制作用(P0.05,P0.01),其作用与阳性对照曲酸相当;白及多糖在浓度0.1~0.8 g/L有较高的抑制黑色素生成作用(P0.05,P0.01);白及多糖浓度0.2~0.4 g/L对TYR、MITF蛋白表达水平有明显抑制作用(P0.05)。结论:白及多糖可能通过抑制TYR的活性和蛋白表达及MITF的蛋白表达,起到抑制黑色素生成的作用。     

放疗计划平面剂量分布验证中不同分辨率对通过率的影响

目的:探讨放疗计划平面剂量分布验证中不同输出分辨率对通过率的影响。方法:选取我院2017年03月至2018年03月收治的调强放疗患者40例,针对患者进行放疗计划平面剂量分布验证,选择不同评价标准（3 mm/3%、2 mm/2%和1 mm/1%）,分析在不同评价标准下Gamma通过率的变化。根据评价标准,分析在不同输出分辨率（0.6 mm、1 mm、2 mm、3 mm、4 mm、5 mm、6 mm）下Gamma通过率的变化情况。选取不同射野（30 cm×30 cm与40 cm×40 cm）,分析Gamma通过率的变化。结果:以3 mm/3%为标准的Gamma通过率高于2 mm/2%、1 mm/1%,且2 mm/2%的Gamma通过率高于1 mm/1%,差异有统计学意义（P＜0.05）。当输出分辨率为3 mm时,无论采用何种评价方式,通过率均有明显下降,其中输出分辨率为4 mm、5 mm、6 mm时的Gamma通过率低于0.6 mm、1 mm、2 mm,差异有统计学意义（P＜0.05）。无论选择何种输出分辨率,3 mm/3%的Gamma通过率始终高于2 mm/2%、1 mm/1%,且2 mm/2%的通过率始终高于1 mm/1%,差异有统计学意义（P＜0.05）。30 cm×30 cm射野下的Gamma通过率高于40 cm×40 cm,差异有统计学意义（P＜0.05）。结论:随着输出分辨率越高,Gamma通过率越低,以3 mm/3%作为评价标准时通过率最高,与40 cm×40 cm射野相比,30 cm×30 cm射野下的通过率更高。     

AN EPIDEMIC OF PLEURISY AMONGST MILITARY RECRUITS

An epidemic amongst recruits who presented with acute viral exudative pleural effusion with lymphocytic pleocytosis is analysed. Histologic and bacteriologic proof of tuberculosis was lacking in majority. Most of them recovered without pleural thickening. Overcrowding, inadequate clothing protection, stress and strain of vigorous recruit training could be important precipitating factors. None reported with parenchymal tuberculosis in two year follow up.KEY WORDS: Pleural effussion, Viral     

纳米级与微米级珍珠粉对大鼠深Ⅱ度烫伤的药效学评价

目的：比较纳米级与微米级珍珠粉对大鼠烫伤皮肤的愈合效果。方法：建立大鼠深Ⅱ度烫伤模型;分别以纳米级珍珠粉高低剂量（30 m g/d,10 m g/d）、微米级珍珠粉高低剂量（30 m g/d,10 m g/d）以及阳性和阴性对照共6组进行对比治疗,测量第3天,第7天和第14天伤痕面积以及各组的痊愈时间;并采用配对t检验法对数据进行统计分析。结果：纳米级珍珠粉对烫伤皮肤的治疗效果要显著优于相应的微米级珍珠粉;纳米级高剂量组（30 m g/d）效果最好,与阳性药物相当;纳米级低剂量组（10 m g/d）的疗效与微米级高剂量组（30 m g/d）相当。结论：珍珠粉纳米化后能显著提高其对烫伤的治疗效果。     

内科胸腔镜在合并胸腔积液肺癌患者分期诊断中的价值

目的 探讨可弯曲内科胸腔镜在合并胸腔积液的肺癌患者临床分期中的应用价值.方法 采用Olympus LTF-240型可弯曲内科电子胸腔镜,对病理已确诊的合并中小量胸腔积液的24例肺癌患者,在分期诊断中行内科胸腔镜检查.24例包括腺癌15例、鳞癌7例、小细胞肺癌2例.结果 内科胸腔镜下可见胸膜病变17例,包括结节13例、扁平隆起2例、胸膜弥漫性充血2例,经内科胸腔镜检查证实为胸膜转移12例,排除胸膜转移12例.15例腺癌有11例确诊为胸膜转移,7例鳞癌1例确诊胸膜转移,2例小细胞肺癌未发现胸膜转移.24例胸腔镜检查均未见严重并发症.结论 内科胸腔镜检查能提高肺癌分期诊断的准确性且安全微创,建议在合并胸腔积液的肺癌患者分期诊断中积极行内科胸腔镜检查.     

神经炎症在阿尔茨海默病发病机制中的作用

阿尔茨海默病(AD)是目前最为常见的一种神经退行性疾病,其病因及发病机制至今还不是很清楚.近几年,越来越多的研究发现,神经炎症在AD的发生发展过程中发挥着重要作用.其中,小胶质细胞的激活及其产生的炎症因子是AD神经炎症的主要表现.此外,在AD疾病过程中,外周侵入脑内的淋巴细胞及炎症因子也参与了AD的神经炎症.因此,研发副作用小的非甾体类抗炎药有望成为防治AD的新靶点.     

冠状动脉临界病变斑块特征与基质金属蛋白酶-9及高敏C-反应蛋白的关系

目的探讨基质金属蛋白酶-9(MMP-9)及高敏C-反应蛋白(hs-CRP)与冠状动脉临界病变斑块特征的关系。方法选择142例临床拟诊为冠心病的患者,进行冠状动脉造影检查,对冠状动脉狭窄在50%～70%的病变行血管内超声检查,根据冠状动脉狭窄及斑块特征,分为正常对照组、稳定斑块组及不稳定斑块组。测定各组血清中MMP-9、hs-CRP浓度,分析斑块特与血清MMP-9、hs-CRP浓度的关系。结果冠状动脉临界病变中有不稳定斑块存在,不稳定斑块组血清中MMP-9、hs-CRP浓度高于稳定斑块组及正常对照组,差异有统计学意义(P<0.05),而稳定斑块组与正常对照组之间MMP-9、hs-CRP浓度差异无统计学意义。结论冠状动脉临界病变中有不稳定斑块存在,血清中MMP-9、hs-CRP浓度增高与斑块的不稳定性有关。