Resistance to fracture of roots obturated with novel canal-filling systems

Introduction

This study was conducted to evaluate and compare the fracture resistance of roots obturated with various contemporary canal-filling systems.

Methods

Fifty single-rooted extracted mandibular premolars were decoronated to obtain 13-mm root segments. Ten roots were left unprepared and unfilled (negative control). Forty canals were instrumented using 0.06 taper EndoSequence files (Brassler, Savannah, GA). Roots were divided according to the obturating system into 4 groups (n = 10): group 1: iRoot SP sealer (Innovative Bioceramix, Vancouver, Canada) + ActiV GP cone (Brasseler USA, Savanah, GA), group 2: iRoot SP sealer + gutta-percha (GP), group 3: ActiV GP sealer + ActiV GP cone, and group 4: ActiV GP sealer + GP. All groups were obturated using single-cone technique. The roots within each group were embedded in acrylic molds and subjected to the fracture resistance test in which a compressive loading (0.5 mm/min) was applied until fracture. Data were statistically analyzed (analysis of variance and Tukey post hoc test).

Results

The significantly highest fracture resistance was recorded for both the negative control group (381.4 ± 53.2 N) and group 1 (iRoot SP sealer/ActiV GP cone [372 ± 62.9 N]) with no significant difference between them, whereas the significantly lowest value was reported in group 4 (ActiV GP sealer/GP cone [288.3 ± 30.5 N]).

Conclusion

Bioceramic-based sealer (ie, iRoot SP) is a promising sealer in terms of increasing in vitro resistance to the fracture of endodontically treated roots particularly when accompanied with ActiV GP cones.     

The mu opioid receptor is involved in buprenorphine-induced locomotor stimulation and conditioned place preference

The analgesic effect of buprenorphine is mediated via the mu opioid receptor (MOP). In the present study, using mice lacking the MOP and their wild-type littermates, we determined the role of the MOP in buprenorphine-induced locomotor stimulation and conditioned place preference (CPP). Buprenorphine (3 mg/kg) increased motor activity in wild-type but not in MOP knockout mice, showing the motor stimulatory action of buprenorphine is mediated via the MOP. When the mice were given the same treatment once daily for 5 consecutive days and challenged with buprenorphine on day 11, the motor stimulatory action of buprenorphine was enhanced in wild-type but not in MOP knockout mice, showing sensitization developed to the motor stimulatory action of buprenorphine and this phenomenon was mediated via the MOP. Likewise, buprenorphine induced CPP in wild-type mice after four alternate-day saline/buprenorphine (3 mg/kg) injections paired with olfactory and visual cues. However, buprenorphine failed to induce CPP in MOP knockout mice. In contrast, amphetamine (1 mg/kg) induced a comparable CPP in wild-type and MOP knockout mice. Together, the present results suggest that the ability of buprenorphine to increase motor activity and induce locomotor sensitization and CPP is mediated via the MOP.