Overexpression of human carcinoma-associated antigen in esophageal adenocarcinoma and its precursor lesions

The expression of human carcinoma-associated antigen (HCA), a mucin-type glycoprotein, was assessed in 50 esophagogastrectomy specimens. Areas, each from different cases, of Barrett esophagus (n = 36), low-grade dysplasia (n = 38), high-grade dysplasia/carcinoma in situ (n = 26), and esophageal adenocarcinoma (EAC; n = 34) were examined by immunohistochemical stains to 2 anti-HCA monoclonal antibodies, G1 and HAE3. These two antibodies showed similar staining patterns. HCA was overexpressed significantly in EAC and high-grade dysplasia/carcinoma in situ compared with benign esophageal mucosa (P < .001 for both), Barrett esophagus (P < .001 for both), and low-grade dysplasia (P < .025 for both). HCA overexpression did not correlate with the grade of EAC (P > .1). The results suggest that overexpression of HCA might help in diagnosing esophageal dysplasia and cancer. The correlation of HCA with the grade of esophageal dysplasia suggests its possible involvement in the pathogenesis of EAC. HCA also might provide a target for immunotherapy.     

角膜移植排斥反应的实验研究（Ⅲ）：环孢霉素A的抗排斥反应

目的探讨环孢霉素A对角膜移植的抗免疫排斥作用。方法以Wistar大鼠异种角膜异位移植为模型，将36只鼠随机分为治疗组及对照组。在术后d2、d5、d7、d10、d14分别取出植片做病理切片，并收集外周血进行T淋巴细胞亚群及T淋巴细胞集落形成数（T-CFU）分析。结果环孢霉素A能推迟排斥反应的发生时间；有明显降低外周血中辅助T淋巴细胞百分率的作用；其对大鼠外周血T淋巴细胞集落形成能力没有明显抑制作用。结论环孢霉素A是一种有效的免疫抑制剂。     

三峡库区青蒿的青蒿素含量的相关和通径分析

目的:通过对影响叶片青蒿素含量主要因素之间的相关性分析及对叶片青蒿素含量的增益大小,为采集高青蒿素含量的野生青蒿提供理论依据。方法:在自然生长条件下,选取三峡库区36个青蒿生长地点,对影响青蒿叶片青蒿素含量相关因素进行相关性和通径分析。结果:土壤中速效P对叶片青蒿素含量的正向直接作用最大,通径系数为0.3439,青蒿植株周围遮阴度对叶片青蒿素含量的负向直接作用最大,通径系数为-0.1421,其它因素的直接作用大小依次为土壤中碱解N(0.3180)、土壤中速效K(0.2352)、生物量(-0.0084)和株高(-0.0347)。结论:叶片青蒿素含量与生物量、遮阴度、土壤中速效K、速效P和碱解N等均存在相关性。     

Determination of a new active steroid by high performance liquid chromatography with laser-induced fluorescence detection following the pre-column derivatization

A sensitive analytical method for the determination of a new active steroid, butane acid-(5-androsten-17-one-3beta-ol)-diester (A1998), was developed by high performance liquid chromatography with laser-induced fluorescence detection following the pre-column derivatization with dansylhydrazine. The calibration curve for A1998 derivatization was found linear in the dynamic range from 0.025 to 5.0 microg/ml, with the precision less than 6% (CV) and the mean extraction efficiency greater than 92%. In 200 microl of plasma samples the limit of quantitation was as low as 0.025 microg/ml with a signal-to-noise ratio of 10. This assaying was further applied to the determination of the pharmacokinetic parameters of A1998 in rats with an intravenous injection of A1998. Values for clearance for elimination, volume of distribution at steady state and terminal half life in the above case were determined as 50.3+/-1.1 ml/min kg, 1329.0+/-111.0 ml/kg and 44.0+/-2.7 min, respectively.     

中医体质量表应用于术后恶心呕吐风险评估的信度和效度分析

【目的】分析中医体质量表的信度和效度。【方法】采用中医体质量表对2013年3月至10月期间符合入组标准的450例女性妇科腹腔镜手术术后恶心呕吐(PONV)患者的体质类型进行了评价,采用Cronbach’sα系数和因子分析初步分析了量表的信度和效度。【结果】184例(40.9%)女性腹腔镜手术PONV患者兼夹2种及以上偏颇体质,阳虚质和气郁质2个分量表具有较好的内部一致性(Cronbach’sα系数分别为0.815和0.803),平和质最低(Cronbach’sα系数为0.514);因子分析提取9个公因子累计方差解释度仅为48.5%。【结论】中医体质量表还需要结合实际应用后的反馈加以修订。     

表皮生长因子对胰腺炎相关蛋白基因表达及胰腺炎的影响

目的 研究表皮生长因子（ＥＧＦ）增强全胃肠外营养（ＴＰＮ）对急性坏死性胰腺炎大鼠胰腺炎相关蛋白（ＰＡＰ）基因表达的影响及其对损伤胰腺细胞的作用。方法 制作大鼠急性坏死性胰腺炎（ＡＮＰ）模型,随机分为：对照组;ＡＮＰ组;ＡＮＰ＋ＴＰＮ组;ＡＮＰ＋ＴＰＮ＋ＥＧＦ组。２４ｈ后动物灌注固定,胰腺组织切片,采用原位杂交组织化学技术结合计算机图像分析,测定胰腺细胞ＰＡＰ－ｍＲＮＡ水平。结果 ＴＰＮ组ＰＡＰ－ｍ     

新型2-芳硫基-5-碘-6-苄基S-DABOs类化合物的合成及作为非核苷类HIV-1 RT抑制剂的活性评估(英文)

我们设计了一系列新型S-DABOs类目标化合物7a-f,通过简单有效的方法合成了该系列化合物,并进行了逆转录酶活性检测,发现该类化合物具有较好的生物活性,其中化合物7d的IC₅₀值达到了3.64μmol/L,是Nevirapine的IC₅₀值的1/2。     

Profile of disposition,tissue distribution and excretion of the novel anti-human immunodeficiency virus (HIV) agent W-1 in rats

The purpose of this study was to characterize the disposition, distribution, excretion and plasma protein binding of 6-benzyl-1-benzyloxymethyl-5-iodouracil (W-1) in rats. Concentrations of W-1 within biological samples were determined using a validated high performance liquid chromatography method. The plasma protein binding of W-1 was examined by equilibrium dialysis method. After oral administration of W-1 (50, 100 and 200 mg/kg, respectively) in self-microemulsifying drug delivery system formulation, the pharmacokinetic parameters of W-1 were as follows: the peak plasma concentrations (C _max) were 0.42, 1.50 and 2.55 μg/mL, the area under the curve (AUC_0?t) were 0.89, 2.27 and 3.96 µg/h mL and the plasma half-life (t _1/2) were 5.15, 3.77 and 3.77 h, respectively. Moreover, the prototype of W-1 was rapidly and extensively distributed into fifteen tissues, especially higher concentrations were detected in intestine, stomach and liver, respectively. The plasma protein binding of W-1 in rat, beagle dog and human were in the range of 97.96–99.13 %. This study suggested that W-1 has an appropriate pharmacokinetics in rats, such as rapid absorption, moderate clearance, and rapid distribution to multiple tissues. Those properties provide important information for further development W-1 as an anti-HIV-1 drug candidate.     

热休克蛋白gp96-肽复合物的提取及纯化

目的：建立从比肝癌细胞中提取、纯化热休克蛋白gp96-肽复合物的方法。方法：①制备H22肝癌细胞蛋白：将H22盘肝癌细胞裂解、离心、过滤及透析后获得。②gp96-肽复合物的纯化：上述可溶性H22肝癌细胞蛋白经分级硫酸铵盐析后，依次用ConA-Sepharose亲和层析、Sephadex G25更换缓冲液及DEAE-Sephacel离子交换层析分离纯化。③gp96-肽复合物的鉴定和浓度测定：所得蛋白经SDS-聚丙烯酰胺凝胶电泳和Western blot进行蛋白分子量及性质鉴定，Lowry法测定蛋白浓度。结果：分离、纯化得到的蛋白经SDS-聚丙烯酰胺凝胶电泳、银染鉴定为单一带，分子量为96kD；Western blot结果证实为HSP gp96。每20ml压积H22细胞最终获得1mg的HSP gp6。结论：使用本分离纯化方法可获得高纯度HSP gp96肽复合物。