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111.
目的:探讨中药对人工晶体状体植入术后虹膜睫状体炎的治疗效果。方法:对24例(29只眼)人工晶状体植入术后虹膜睫状体炎患者,在后马托品或托品酰胺滴眼散瞳的情况下,用口服血栓通胶囊和凉血清热泻肝汤加减进行了治疗和为期3个月 ̄12个月的随访观察。结果:治疗时间最短为5天,最长者21天,平均11天。治愈22例27只眼(93.10%),未见复发,视力达0.6以上。好转2例2只眼(6.9%)。结论:在应用散瞳 相似文献
112.
Inhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenols 总被引:47,自引:7,他引:47
In order to study the biological activities of tea preparations and
purified tea polyphenols, their growth inhibitory effects were investigated
using four human cancer cell lines. Growth inhibition was measured by
[3H]thymidine incorporation after 48 h of treatment. The green tea
catechins (-)-epigallocatechin-3-gallate (EGCG) and (-)- epigallocatechin
(EGC) displayed strong growth inhibitory effects against lung tumor cell
lines H661 and H1299, with estimated IC50 values of 22 microM, but were
less effective against lung cancer cell line H441 and colon cancer cell
line HT-29 with IC50 values 2- to 3- fold higher.
(-)-Epicatechin-3-gallate, had lower activities, and (-)- epicatechin was
even less effective. Preparations of green tea polyphenols and theaflavins
had higher activities than extracts of green tea and decaffeinated green
tea. The results suggest that the growth inhibitory activity of tea
extracts is caused by the activities of different tea polyphenols. Exposure
of H661 cells to 30 microM EGCG, EGC or theaflavins for 24 h led to the
induction of apoptosis as determined by an annexin V apoptosis assay,
showing apoptosis indices of 23, 26 and 8%, respectively; with 100 microM
of these compounds, the apoptosis indices were 82, 76 and 78%,
respectively. Incubation of H661 cells with EGCG also induced a
dose-dependent formation of H2O2. Addition of H2O2 to H661 cells caused
apoptosis in a manner similar to that caused by EGCG. The EGCG-induced
apoptosis in H661 cells was completely inhibited by exogenously added
catalase (50 units/ml). These results suggest that tea polyphenol-induced
production of H2O2 may mediate apoptosis and that this may contribute to
the growth inhibitory activities of tea polyphenols in vitro.
相似文献
113.
Characterization of xenobiotic-metabolizing enzymes and nitrosamine metabolism in the human esophagus 总被引:5,自引:2,他引:5
Smith TJ; Liao A; Wang LD; Yang GY; Starcic S; Philbert MA; Yang CS 《Carcinogenesis》1998,19(4):667-672
Esophageal cancer has been associated with tobacco smoking, and
nitrosamines are possible causative agents for this cancer. The present
study investigated the metabolism of the tobacco carcinogens N'-
nitrosonornicotine (NNN), 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone
(NNK), and N-nitrosodimethylamine (NDMA), as well as the presence of
xenobiotic-metabolizing enzymes in human esophageal tissues from
individuals in the United States and Huixian, Henan Province, China (a
high-risk area for esophageal cancer). All esophageal microsomal samples
activated NNN and the metabolic rate was 2-fold higher in the esophageal
samples from China than the USA. All microsomal samples activated NDMA.
However, most of the microsomal samples did not activate NNK.
Troleandomycin (an inhibitor of cytochrome P450 3A) decreased the formation
of NNN-derived keto acid by 20-26% in the esophageal microsomes. The
activities for NADPH: cytochrome c reductase, ethoxycoumarin O-deethylase,
NAD(P)H: quinone oxidoreductase and glutathione S-transferase were present
in the esophageal samples. Coumarin 7-hydroxylase (a representative
activity for P450 2A6) activity was not detected in the esophageal
microsomal samples. The activities for nitrosamine metabolism and
xenobiotic- metabolizing enzymes were decreased (by 30-50%) in the squamous
cell carcinomas compared with their corresponding non-cancerous mucosa. The
presence of activation and detoxification enzymes in the esophagus may play
an important role in determining the susceptibility of the esophagus to the
carcinogenic effect of nitrosamines. Our results suggest that P450s 3A4 and
2E1 are involved in the activation of NNN and NDMA, respectively, in the
human esophagus.
相似文献
114.
Early stage nasopharyngeal carcinoma: radiotherapy dose and time factors in tumor control 总被引:1,自引:0,他引:1
Chang JT; See LC; Liao CT; Chen LH; Leung WM; Chen SW; Chen WC 《Japanese journal of clinical oncology》1998,28(3):207-213
OBJECTIVE: To evaluate radiotherapy dose and length of treatment in the
control of early stage nasopharyngeal carcinoma (NPC) treated with a
combination of external radiotherapy and brachytherapy, MATERIALS &
METHODS: We reviewed the records of 133 patients with early stage
nasopharyngeal carcinoma (stage I or II, AJC/UICC staging system) who
received definitive radiotherapy in Chang Gung Memorial Hospital from 1979
to 1991. The median follow-up time was 7.1 years with a minimum of 2 years.
All patients were treated with megavoltage external radiotherapy to the
nasopharynx area (63-72 Gy) followed by high dose rate intracavitary
brachytherapy (5-16.5 Gy in one to three fractions, spaced 1-2 weeks
apart). The median total dose and time of irradiation was 75 Gy (69.8-81.4
Gy) and 11.6 weeks (7.8-20 weeks) respectively. Survival analysis was used
to examine the effect of several variables on prognosis. RESULTS: The
5-year rates were 86.4% for local control, 84.7% for disease free survival,
88.5% for actuarial survival and 84.2% for overall survival. The treatment
group (combination of time and dose of irradiation) was the most important
prognostic factor according to Cox's proportional hazard model. Patients
receiving radiation at a total dose of < or = 75 Gy completed in < 12
weeks showed the best prognosis. CONCLUSION: Treatment time and total
treatment dose are both important factors in treating early stage NPC.
Decreasing the total radiation time to < 12 weeks and not exceeding a
radiation dose of 75 Gy gave the best results.
相似文献
115.
E. R. Sherwood J. L. Van Dongen C. G. Wood S. Liao J. M. Kozlowski C. Lee 《British journal of cancer》1998,77(6):855-861
These studies were undertaken to assess the relative expression and autocrine activation of the epidermal growth factor receptor (EGFR) in normal and transformed prostatic epithelial cells and to determine whether EGFR activation plays a functional role in androgen-stimulated growth of prostate cancer cells in vitro. EGFR expression was determined by Western blot analysis and ELISA immunoassays. Immunoprecipitation of radiophosphorylated EGFR and evaluation of tyrosine phosphorylation was used to assess EGFR activation. The human androgen-independent prostate cancer cell lines PC3 and DU145 exhibited higher levels of EGFR expression and autocrine phosphorylation than normal human prostatic epithelial cells or the human androgen-responsive prostate cancer cell line LNCaP. PC3 and DU145 cells also showed higher levels of autonomous growth under serum-free defined conditions. Normal prostatic epithelial cells expressed EGFR but did not exhibit detectable levels of EGFR phosphorylation when cultured in the absence of exogenous EGF. Addition of EGF stimulated EGFR phosphorylation and induced proliferation of normal cells. LNCaP cells exhibited autocrine phosphorylation of EGFR but did not undergo significant proliferation when cultured in the absence of exogenous growth factors. A biphasic growth curve was observed when LNCaP cells were cultured with dihydrotestosterone (DHT). Maximum proliferation occurred at 1 nM DHT with regression of the growth response at DHT concentrations greater than 1 nM. However, neither EGFR expression nor phosphorylation was altered in LNCaP cells after androgen stimulation. In addition, DHT-stimulated growth of LNCaP cells was not inhibited by anti-EGFR. These studies show that autocrine activation of EGFR is a common feature of prostatic carcinoma cells in contrast to normal epithelial cells. However, EGFR activation does not appear to play a functional role in androgen-stimulated growth of LNCaP cells in vitro. 相似文献
116.
低剂量粒—巨噬细胞集落刺激因子在肺癌化疗中的应用 总被引:2,自引:0,他引:2
31例肺癌患者大剂量化疗40例次,采用配对法分成加用低剂量粒-巨噬细胞集落刺激因子(GM-CSF)的治疗组及不加用的对照组(二组均为20例次)。结果表明,低剂量GM-CSF明显缩短化疗所致白细胞低下的时间:治疗组为14.5±9.49天,对照组为19.4±8.85天(P=0.02),同时提高白细胞下降最低值:治疗组为3.35×109/L±1.37×109/L,对照组为2.9×109/L±1.18×109/L。低剂量GM-CSF的主要不良反应为发热(80%)及肌痛、骨痛(10%)。结果提示:低剂量GM-CSF能支持癌症患者的大剂量化疗及连续化疗 相似文献
117.
目的:研究绞股蓝(GP)对庆大霉素(GM)引起的近端肾小管细胞(PTC)毒性的保护作用。方法:采用原代培养的 PTC 制作 GM 损伤模型,观察丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)、N-乙酰-β-氨基葡萄糖酶(NAG)、过氧化氢酶(catalase)、DNA 合成以及形态学变化,同时观察 GP 的保护作用。结果:GP 能激活并保护 ALT、LDH、Catalase 酶活力,降低 NAG酶活力增高、促进 DNA 合成等机制,减轻 GM 的肾毒性损伤。结论:GP 能有效保护 GM 对PTC 损伤。 相似文献
118.
119.
腔内超声对直肠癌术前分期诊断的应用价值 总被引:6,自引:1,他引:6
目的:评价腔内超声(ELUS)对直肠癌术前分期诊断的准确性及局限性.方法:对58例直肠癌术前行ELUS检查,参考TNM分期标准进行术前分期诊断,并与手术及术后病理结果对照.结果:ELUS对58例直肠癌浸润深度诊断符合率达79.3%,T1、T2、T3、T4各期诊断灵敏度分别为100%、58.8%、87.5%、83.3%.对T2期诊断灵敏度最低,误诊7例中6例过深判断为T3期,且均为溃疡型腺癌.ELUS对54例直肠癌淋巴结转移诊断灵敏度、特异度、准确度分别为76.9%、75.0%、75.9%.淋巴结转移ELUS漏诊6例,淋巴结转移ELUS诊断假阳性7例.结论:ELUS对直肠癌浸润深度及肠周淋巴结转移诊断准确度较高,可成为直肠癌术前分期诊断良好的方法.对T2期的过深判断为影响诊断符合率的重要因素,肿瘤导致肠腔明显狭窄或肿瘤位于直肠上段也影响ELUS的准确性. 相似文献
120.
不同月龄正常大鼠骨形态计量学参数动态变化的研究 总被引:3,自引:0,他引:3
目的 :探讨不同月龄正常大鼠骨形态计量学参数变化 ,为抗骨质疏松药物研究提供对照依据。方法 :4 .5月龄SD雌性大鼠 ,按体重随机分组 ,在实验的d 0 (4.5月龄 )、d 30 (5 .5月龄 )、d 75 (7月龄 )、d 14 0 (10月龄 )杀死大鼠取材 ,采用体内双荧光标记法 ,胫骨上段硬组织包埋切片及松质骨形态计量学分析 ,观察不同月龄段大鼠的骨变化情况。结果 :正常大鼠 4 .5~ 10月龄 ,骨量有一缓慢上升后下降的过程 ,但变化辐度不大 (P >0 .0 5 ) ,骨量相对稳定 ,骨形成和骨吸收有先上升后下降的变化过程 (P <0 .0 5 )。结论 :4 .5~ 10月龄的SD雌性大鼠体重和骨计量学参数变化正常 ,选择该月龄段大鼠做模型 ,研究药物对骨质疏松的防治是可行的。 相似文献