非甾体抗生育药物的研究 Ⅱ.对-羟基苯丙烯酸衍生物的合成及其抗生育活性

据报道,对-羟基本丙烯酸(Ⅰ)对小鼠有很好的抗生育作用。鉴于芳基取代的苯乙烯美化合物亦有不同程度的抗生育活性,我们在对-羟基苯丙烯酸的α位,用芳基取代,合成了24个衍生物。初步药理筛选结果表明,化合物Ⅱ_(1,3,7,23)对小鼠有抗早孕作用(P<0.05～0.001),其中以化合物Ⅱ_1为显著(P<0.001)。     

Prognostic significance of excision repair cross complementation group 1 rs2298881 in patients with gastric cancer receiving platinum-based chemotherapy: A PRISMA-compliant meta-analysis

Background:Gastric cancer (GC) is a strong cause of global cancer mortality. Nucleotide excision repair (NER) can modulate platinum-based chemotherapeutic efficacy by removing drug-produced DNA damage. Some studies have found a link between excision repair cross complementation group 1 (ERCC1) rs2298881, one gene in NER pathway, and response to chemotherapy. However, the results have been disputed.Methods:We conducted a meta-analysis to reevaluate the association between polymorphisms of NER gene (ERCC1 rs2298881) and the clinical outcomes in gastric cancer patients receiving platinum-based chemotherapy. Searching PubMed, Web of Science, EMBASE, Google Scholar, and China National Knowledge Infrastructure, 2 independent searchers found all pertinent literatures up to May 1, 2021. We enrolled studies according to consistent selection criteria, extracted and vitrified data. Crude odds ratios (ORs) and hazard ratios (HRs) with 95% confidence interval (CI) were applied to evaluate the effect of ERCC1 rs2298881 on patients treated by platinum-based chemotherapy.Results:By the data gathered from 6 independent studies, 1940 cases diagnosed with gastric cancer and treated with chemotherapy were included, containing 1208 Good-Responders and 732 Poor-Responders. With a comprehensive meta-analysis, we found that the patients with ERCC1 rs2298881A allele had a worse response to chemotherapy than those who with rs2298881C allele under allelic model (A vs C), with the pooled OR of 0.780 (95% CI: 0.611–0.996, P = .046). And our analysis indicated that AA genotype was associated with unfavorable overall survival (HR = 1.540, 95% CI = 1.106–2.144, P = .011) compared with CC genotype.Conclusions:ERCC1 rs2298881 is suggested as a marker of clinical outcome in gastric cancer patients treated by platinum-based chemotherapy.     

深化对角膜屈光手术视觉质量重要性的认识

近年来,角膜屈光手术在我国得到了快速的发展,其安全性及有效性一直是患者和医生关注的重点.随着理论与技术的发展,角膜屈光手术的安全性已较普遍地得到了良好保证,人们对手术的质量日益关注.视觉质量改善程度成为反映角膜屈光手术质量的重要指标,笔者阐述了视觉质量的评估意义与方法,并对如何保障角膜屈光手术后的视觉质量提出看法,希望借此深化对角膜屈光手术视觉质量重要性的认识.     

Development of a loop-mediated isothermal amplification method for rapid detection of caprine arthritis-encephalitis virus proviral DNA

A rapid detection assay based on loop-mediated isothermal amplification (LAMP) has been developed for detecting caprine arthritis-encephalitis (CAEV) proviral DNA. The LAMP assay utilized a set of five primers designed against highly conserved sequences located within the p25 gene region. The assay successfully detected CAEV proviral DNA in total DNA extracts originating from cell culture, whole blood samples and separated PBMCs. There was no cross-reaction with the negative control. Amplification was monitored using a Loopamp real-time turbidimeter; turbidity and the corresponding time were recorded. Amplification from CAEV-Shanxi DNA was detected as early as 17 min, with a maximum sensitivity of 0.0001 TCID(50), reached at 32 min. Sixty-eight animal blood samples were tested using AGID, PCR and LAMP assay, and the positive rates were 30.9 %, 33.8 % and 47.1 %, respectively. Whole blood can be used directly, eliminating the need for separation of PBMCs and nucleic acid extraction, reducing the overall procedure time to approximately 80 min. Therefore, the LAMP assay provides a specific and sensitive means for detecting CAEV proviral DNA in a simple, fast, and cost-effective manner and should be useful in eradication programs and epidemiological studies. Furthermore, the LAMP assay can be performed in less-well-equipped laboratories as well as in the field.     

CD44、NFYB及HMGB1基因表达与乳腺癌术后复发关系的研究

目的 研究黏附分子CD44、核转录因子Y,β（NFYB）及高迁移率蛋白B1（high-mobility group box-1,HMGB1）基因表达与乳腺癌术后复发转移的关系,初步探讨其在判断乳腺癌预后的价值.方法 81例乳腺癌患者入选,其中41例术后5年随访期内出现复发者为研究组;40例未复发仍存活者为对照组.提取所有病例石蜡包埋组织RNA,质量鉴定后,应用实时定量逆转录-聚合酶链反应（RT-PCR）芯片技术检测CD44 、NFYB及 HMGB1基因的表达,分析2组间基因表达差异,进行多因素分析.结果 研究组与对照组相比较,CD44 、NFYB及 HMGB1基因表达有差异,表达上调（P〈0.05）.将基因表达与术后复发时间进行COX分析,结果显示CD44 基因表达与乳腺癌术后复发时间具有负相关（B=-0.042,Exp（B）=0.959）,NFYB及HMGB1基因表达与乳腺癌术后复发时间无相关性,结果无统计学意义.结论 CD44 、NFYB及 HMGB1基因在乳腺癌术后复发组和未复发组中差异表达;基因CD44 过表达是乳腺癌预后差的指标之一,进一步研究可为预防和控制乳腺癌的复发提供新线索.     

新型毒品滥用对男男性行为人群高危性行为的影响

目的 了解新型毒品滥用对MSM高危性行为及HIV感染的影响,为制订有针对性的艾滋病防治对策和干预措施提供依据。方法 2019年4-7月在山东省3个MSM监测哨点,收集当地MSM人口学特征、相关行为信息,采集血样做HIV和梅毒检测。结果 共调查MSM 1 203人,年龄（30.2±8.9）岁,未婚815人（67.7%）,大专及以上文化程度805人（66.9%）;曾经使用过新型毒品的比例为54.2%（652/1 203）;最近6个月发生过无保护肛交和群交行为的比例分别为62.2%（721/1 160）和16.3%（189/1 160）;HIV和梅毒抗体阳性率分别为3.1%（37/1 203）和4.7%（56/1 203）。多因素logistic回归分析结果显示,MSM最近6个月发生无保护性肛交的影响因素中,来自青岛市是济南市的1.59倍（95% CI：1.13～2.23）、高中及以下文化程度是大专及以上文化程度的1.61倍（95% CI：1.20～2.16）、从酒吧/歌舞厅等场所寻找男性性伴的是互联网/交友软件的1.69倍（95% CI：1.06～2.69）、艾滋病知识得分<6分是≥6分的1.55倍（95% CI：1.01～2.37）、最近1周同性性伴数≥2人是<2人的1.48倍（95% CI：1.12～1.95）、最近6个月发生群交行为是无群交行为的2.43倍（95% CI：1.61～3.67）,曾使用新型毒品是未使用毒品的1.46倍（95% CI：1.11～1.91）。MSM最近6个月发生群交行为的影响因素中,来自青岛市是济南市的2.21倍（95% CI：1.45～3.38）、外省户籍是本省户籍的2.13倍（95% CI：1.28～3.55）、艾滋病知识得分≥6分是<6分的3.11倍（95% CI：1.55～6.25）、最近6个月肛交未坚持使用安全套是坚持使用安全套的2.60倍（95% CI：1.71～3.96）、最近6个月有同性商业性行为是无同性商业性行为的3.38倍（95% CI：1.92～5.95）、曾经使用过新型毒品是未使用毒品的2.71倍（95% CI：1.82～4.04）、最近1年未检测HIV是检测HIV的2.66倍（95% CI：1.59～4.43）。结论 山东省MSM人群中使用新型毒品现象普遍,新型毒品滥用促进该人群无保护性行为和群交行为的发生,应重点加强对吸毒MSM监测和干预力度。     

腓血管为蒂的胫腓骨远段后面骨膜瓣移位术

目的：为胫骨中、下段骨不连合并胫前区软组织条件极差者提供一种新的疗法。方法：应用经小腿后路以腓血管为蒂的胫腓骨远段后面骨膜瓣移位予以修复。结果：临床应用６例，效果满意。结论：本骨膜瓣移位术具有操作简便、手术安全、疗效可靠和易于推广等优点     

二级医院的卒中患者延续护理体系构建

目的 针对卒中后残疾患者出院后的随访和护理干预对康复的影响,构建延续护理体系,提高患者自理能力和生存质量.方法 通过抽取3家社区卫生服务中心为主要调查点,以脑卒中后残疾患者为调查对象,对服务现状进行分析,提出上海市某二级医院构建延续护理体系的建议.结果 ①患者依从性:出院指导依从性总分(t=6.011,P=0.000<0.05)及其中的3个单项依从性康复训练(t=5.255,P=0.000<0.05)、生活作息(t=5.156,P=0.000<0.05)、复诊随访(t=3.476,P=0.010<0.05)显著高于对照组;均衡饮食(t=0.967,P=0.337>0.05)和正规服药(t=0.845,P=0.401>0.05)2个单项无显著差异.②卫生服务利用率:干预组门诊访问率显著高于对照组(χ2=9.053,P=0.029<0.05);两组的再次住院率(χ2=0.278,P=0.870>0.05)和急诊访问率(χ2=1.036,P=0.596>0.05)无显著差异.结论 二级医院脑卒中患者延续护理体系的构建显示出积极效果,达到方案设计的目的.