Two dinucleotide repeat polymorphisms at the D8S1442 and D8S1443 loci

Two polymorphic dinucleotide (CA) repeat clones were isolated from cosmids, cCI8-1121 and cCI8-1199, mapped to chromosome 8p11.2-p12.     

Ex vivo stimulation of cord blood mononuclear cells by dexamethasone and interleukin-7 results in the maturation of interferon-gamma-secreting effector memory T cells

The effects of dexamethasone phosphate and interleukin‐7 upon the proliferation of T‐cells and the production of interferon‐γ in the newborn's cord blood mononuclear cell cultures were studied. The capability of dexamethasone to enhance T‐cell proliferation induced by anti‐CD3 with interleukin‐7 in some newborn cord blood mononuclear cell cultures was identified. Dexamethasone suppressed production of interferon‐γ in 68‐h cell cultures stimulated with anti‐CD3 both in the presence of interleukin‐7 and without it. However, a 68‐h cultivation of newborn blood cells with dexamethasone, anti‐CD3 and interleukin‐7 resulted in the accumulation of T‐lymphocytes capable of producing interferon‐γ after restimulation. As a result of it the amount of interferon‐γ producing CD7⁺ T‐cells and the concentration of interferon‐γ in cultural supernatants were maximal in the cell cultures incubated with anti‐CD3, interleukin‐7 and dexamethasone during the first 68 h and subsequently restimulated with phorbol 12‐myristate 13‐acetate and ionomycin. The stimulation of neonatal or adult blood cells by dexamethasone, anti‐CD3 and interleukine‐7 also causes a decrease in the number of naïve T‐cells and central memory cells and an increase in the number of effector memory CD7⁺CD45RA⁺CD62L^– cells in cultures. It is possible that these effects are caused by the influence of dexamethasone on IL‐7 receptor expression: it is known that IL‐7 receptor alpha‐chain gene is a glucocorticoid‐inducible gene.     

巢蛋白和阶段特异性胚胎抗原-1在大鼠2型星形胶质细胞中的表达

目的 观察1型和2型星形胶质细胞(T1A、T2A)是否表达神经干细胞的标志物、是否具有神经干细胞的特性.方法 取新生大鼠脑皮质,体外培养纯化的O-2A祖细胞、T1A和T2A,应用激光共焦双重免疫荧光标记技术检测巢蛋白和阶段特异性胚胎抗原-1(SSEA-1)的表达;观察O-2A祖细胞、 T1A和T2A在碱性成纤维生长因子(bFGF)和表皮生长因子(EGF)的培养液中生长方式的改变.结果 巢蛋白在O-2A祖细胞和T2A中表达,T1A不表达;SSEA-1仅在T2A中表达.在干细胞培养基中培养10d,T2A形成能增殖和连续传代的细胞球,细胞球巢蛋白标记阳性,贴壁后分化细胞具有神经元、星形胶质细胞和少突胶质细胞样形态;但相同培养条件下的O-2A祖细胞和T1A生长方式无改变.结论 巢蛋白和SSEA-1在两型星形胶质细胞中的表达存在差异,T2A具有神经干细胞的某些生物学特性.     

Syndiotactic polymerization of styrene with cyclopentadienyltribenzyloxytitanium/methylaluminoxane catalyst

Syndiotactic polystyrene was efficiently prepared in the presence of a homogeneous catalyst composed of (η⁵-cyclopentadienyl)tribenzyloxytitanium and methylaluminoxane (MAO) as a cocatalyst. Influences of various polymerization conditions, e.g., [Ti]. [MAO], [St], temperature and the content of retained trimethylaluminium (TMA) in MAO on the catalytic activity, syndiotacticity and molecular weight of the polymer were studied. It was found that the retained TMA plays an important role in the polymerization. Lowering the retained TMA content in MAO decreases the activity of the catalyst remarkably. Addition of external alkylaluminium (TMA or triisobutylaluminium) into the catalyst system with MAO containing low amounts of retained TMA promotes styrene polymerization.     

肢端黏液样炎性纤维母细胞肉瘤2例及文献复习

目的探讨肢端黏液样炎性纤维母细胞肉瘤的临床病理学特征及鉴别诊断。方法对2例发生在下肢末端的黏液样炎性纤维母细胞肉瘤进行光镜观察和免疫组化标记，并复习文献。结果2例发生在下肢末端的病程较长的渐进性肿块，术后局部复发。镜检：病变呈多结节状，边界不清；黏液样基质中见数量不等的各类炎细胞浸润，散在或灶性分布梭形、奇异形和多空泡状脂肪母细胞样3种形态的瘤细胞。免疫表型：肿瘤细胞Vim弥漫阳性，CD68和CD34灶性阳性，CK、SMA、HHF-35、S-100蛋白、CD45、CD45R0、CD15、CD30均阴性。结论此病病程较长，术后易局部复发，是一种低度恶性的肿瘤。鉴于病变黏液样基质及各类炎细胞浸润的背景较为突出，而特征性的瘤细胞稀疏，应注意与炎症性病变或具有相似组织形态的良性或恶性肿瘤鉴别。     

自血光量子疗法治疗病毒性肝炎72例疗效观察

1993年9月以来,用自血光量子治疗各型病毒性肝炎72例,结果所有患者症状均明显好转或消失,SB异常者恢复正常和有效分别占66.7％和90.2％,ALT异常者恢复正常和有效分别为77.4％和100％,血清白蛋白平均上升4.2g/L,球蛋白下降4.6g/L,三项凝血功能(PT、HPT、KPTT)测定结果表明量子血疗不会引起或加重肝炎患者的凝血障碍。61例乙型肝炎患者HBeAg和HBsAg的阴转率分别达59.3％和15％。     

Attenuation of intracavitary applicators in 192Ir-HDR brachytherapy

Unlike the penetrating monoenergetic 662 keV gamma rays emitted by 137Cs LDR sources, the spectrum of 192Ir used in HDR brachytherapy contains low-energy components. Since these are selectively absorbed by the high-atomic number materials of which intracavitary applicators are made, the traditional neglect of applicator attenuation can lead to appreciable dose errors. We investigated the attenuation effects of a uterine applicator, and of a set of commonly used vaginal cylinders. The uterine applicator consists of a stainless steel source guide tube with a wall thickness of 0.5 mm and a density of 8.02 g/cm3, whereas the vaginal cylinders consist of the same stainless steel tube plus concentric polysulfone cylinders with a radius of 1 or 2 cm and a density of 1.40 g/cm3. Monte Carlo simulations were performed to compute dose distributions for a bare 192Ir-HDR source, and for the same source located within the applicators. Relative measurements of applicator attenuation using ion-chambers (0.125 cm3) confirmed the Monte Carlo results within 0.5%. We found that the neglect of the applicator attenuation overestimates the dose along the transverse plane by up to 3.5%. At oblique angles, the longer photon path within applicators worsens the error. We defined attenuation-corrected radial dose and anisotropy functions, and applied them to a treatment having multiple dwell positions inside a vaginal cylinder.     

化合物构效关系的模糊极小极大神经网络识别研究

应用模糊极小极大神经网络研究了化合物复杂结构和性能（QSAR）之间的关系，用该法进行几组化合物致癌的识别，结果优于线性回归的方法，对此作出一些分析。