Relationship Between Genetic Polymorphisms in the HTR1A Gene and Paranoid Schizophrenia in a Northern Han Chinese Population

The hypothesis for the etiology of schizophrenia involves various neurotransmitters, including 5-HT. Metabolic disorder of 5-HT is an important underlying neurobiochemical cause leading to the development of mental illness. Abnormality in the receptors involved in 5-HT synthesis and metabolism may affect the functioning of 5-HT in the central nervous system. There are seven types of 5-HT receptor families, with a total of 15 corresponding subtypes. HTR1A is the most abundantly expressed 5-HT receptor subtype in the mammalian brain. SNPs in HTR1A enhance or weaken the functioning of 5-HT by affecting HTR1A expression levels or ligand-binding activity, thereby placing HTR1A in an important role in the study of diseases of the nervous system. This study employed DNA sequencing to investigate HTR1A fragment lengths, including complete exons as well as 5′ FR and 3′ FR segments, for a total of 2,718 bp. Seven SNP loci (ss212928868, rs6295, rs6294, ss218178047, rs34118353, rs6449693, and rs878567) were found in 182 healthy volunteers and 161 patients. Among them, two SNP loci had not been reported in the National Center for Biotechnology Information (NCBI) database, promoter locus ss212928868 and exon locus ss218178047, which now have been approved by the NCBI database and assigned rs numbers, rs113195492 and rs112846276, respectively. ss212928868 and rs6294 were statistically different between control and paranoid schizophrenic women (P?<?0.05), and both loci were in a state of linkage disequilibrium. However, statistical significance was lost after Bonferroni correction. Compared with the GG genotype, the GA + AA genotype had a decreased disease risk (odds ratio_{GA + AA}?=?0.3529, 95 % confidence interval?=?0.1319–0.9444). The data showed that changes in SNP loci of HTR1A were different between paranoid schizophrenic and control group women. Although such differences were lost after statistical correction, studies with larger sample sizes have not been conducted. Combined with the newly discovered loci, these findings can point out possible directions for future investigations in different populations.     

Quality evaluation of systematic reviews of the effects of Baduanjin on diabete

ObjectiveRecently, the effects of Baduanjin (a traditional Chinese mind-body exercise) on diabetes have attracted increasing attention, and relevant systematic reviews (SRs) have emerged. However, the qualities of these SRs vary markedly, and their conclusions are inconsistent, which is not conducive to guiding decision-making. We sought to assess the quality of these SRs and provide more evidence for diabetes treatment.MethodsA literature search was conducted in 7 databases from inception to December 1_st, 2021. SRs of randomized controlled trials investigating the effects of Baduanjin were identified. A Measurement Tool to Assess Systematic Reviews 2 (AMSTAR-2) checklist, Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) statement, and Grading of Recommendations Assessment, Development and Evaluation (GRADE) system were applied to evaluate the quality of the SRs.ResultsTen SRs were identified. All SRs were judged as critically low methodological quality by the AMSTAR-2 checklist. The total PRISMA score of the included SRs ranged from 15 to 21, and the mean score was 18.60 (1.90), indicating that all SRs had partial reporting deficits. Sixty outcomes were reported in the included studies, of which 25 (41.67%) were judged as low quality, and 34 (56.67%) as very low quality according to the GRADE system, indicating that the overall evidence quality of outcomes was not high.ConclusionBaduanjin seems to be an effective therapy for diabetes in improving glucose and lipid metabolism, mental health, quality of life, and waist-hip ratio (WHR). The overall quality of the SRs was less than optimal. This conclusion should be treated with caution, and researchers should conduct higher-quality clinical studies following AMSTAR-2checklist, PRISMA statement, and GRADE system in the future.     

普通胸外科临床见习教学的特点及带教手段

临床见习是临床实践教学的重要组成部分，是培养和提高医学生应用所学理论知识进行逻辑思维与临床实践综合运用能力的重要阶段，是实现医学生向实习医生角色转变的必须过程，同时也是提高医学生理论知识水平和临床实践技能的传统教育模式之一，在医学教学中起着承前启后的作用。使医学生较好地完成从学生到医生、从书本到病房、从校园到社会之间这一重要过渡阶段。     

长效β2受体激动剂班布特罗对哮喘患者肺功能影响的观察

目的：了解班布特罗对哮喘患肺功能的影响。方法：选择50例患，治疗组（25例），给予班布特罗20mg每晚1次口服。对照组（25例），给予美喘清50μg日2次口服，疗程4周，随访2周，以用药前后PEF、FEV1、FVC变化为观察指标，结果：治疗组用2周肺功能即有改善，清晨PEF显提高（P＜0．05），用药4周时改变最明显（P＜0．01），对照组用药后清晨PEF稍有提高，但与用药相比差别不显，结论：班布特罗睡前常规服用可明显提高PEF，控制夜间哮喘发作，是安全而有效的长效支气管扩张药物。     

Study on the Genotype of Extended-spectrum Beta-lactamases Mediated by Plasmid in Southern China

Alongwiththewidespreaduseoftheextended spectrum beta lactamase(ESBL)antibioticswithinlast20years, theemergenceofESBLs producingstrainsamongmembers ofthefamilyEnterobacteriaceaehasbecomeaworldwide problem.TheESBLsdescribedtodatebelongtotheBush group2orAmbl…     

稳定过表达成纤维细胞激活蛋白的口腔鳞状细胞癌细胞株的构建

目的 构建人成纤维细胞激活蛋白（FAP）过表达慢病毒载体,转染口腔鳞状细胞癌（OSCC）细胞株SCC9,构建稳定过表达FAP的OSCC细胞株。方法 采用聚合酶链反应（PCR）技术获得FAP片段,利用基因重组技术构建过表达FAP的慢病毒载体,包埋病毒并收集上清液感染SCC9细胞株,通过流式细胞荧光分选技术（FACS）进行筛选,获得稳定过表达FAP的SCC9细胞株。结果 对阳性克隆进行酶切鉴定和基因测序证明FAP的慢病毒载体构建成功,实时荧光定量PCR和Western blot检测结果证明成功构建稳定过表达FAP的SCC9细胞株。结论 本研究有助于获得纯化FAP蛋白,为进一步研究FAP在OSCC发生发展过程中的机制奠定基础。     

Sox17 Promoter Methylation in Plasma DNA Is Associated With Poor Survival and Can Be Used as a Prognostic Factor in Breast Cancer

Aberrant DNA methylation that leads to the inactivation of tumor suppressor genes is known to play an important role in the development and progression of breast cancer. Methylation status of cancer-related genes is considered to be a promising biomarker for the early diagnosis and prognosis of tumors. This study investigated the methylation status of the Sox17 gene in breast cancer tissue and its corresponding plasma DNA to evaluate the association of methylation levels with clinicopathological parameters and prognosis.The methylation status of the Sox17 gene promoter was evaluated with methylation-specific polymerase chain reaction (MSP) in 155 paired breast cancer tissue and plasma samples and in 60 paired normal breast tissue and plasma samples. Association of Sox17 methylation status with clinicopathological parameters was analyzed by χ² tests. Overall and disease-free survival (DFS) curves were calculated using Kaplan–Meier analysis, and the differences between curves were analyzed by log-rank tests.The frequency of Sox17 gene methylation was 72.9% (113/155) in breast cancer tissues and 58.1% (90/155) in plasma DNA. Sox17 gene methylation was not found in normal breast tissues or in their paired plasma DNA. There was a significant correlation of Sox17 methylation between corresponding tumor tissues and paired plasma DNA (r = 0.688, P < 0.001). Aberrant Sox17 methylation in cancer tissues and in plasma DNA was significantly associated with the tumor node metastasis stage (P = 0.035 and P = 0.001, respectively) and with lymph node metastasis (P < 0.001 and P = 0.001, respectively). Kaplan–Meier survival curves showed that aberrant Sox17 promoter methylation in cancer tissues and plasma DNA was associated with poor DFS (P < 0.005) and overall survival (OS) (P < 0.005). Multivariate analysis showed that Sox17 methylation in plasma DNA was an independent prognostic factor in breast cancer for both DFS (P = 0.020; hazard ratio [HR] = 2.142; 95% confidence interval [CI]: 1.128–4.067) and for OS (P = 0.001; HR = 4.737; 95% CI: 2.088–10.747).Sox17 gene promoter methylation may play an important role in breast cancer progression and could be used as a prognostic biomarker to identify patients at risk of developing metastasis or recurrence after mastectomy.     

食管癌根治术中综合保温干预对患者术后寒战及炎症应激反应的影响

目的研究食管癌根治术中采取综合保温干预对患者术后寒战及炎症应激反应的影响。方法将40例手术患者分为观察组和对照组各20例,对照组予常规保温措施,观察组在对照组基础上加用循环水变温毯持续加温,观察两组患者术中各时间点体温及术后寒战发生情况和C反应蛋白、白介素-6的变化情况。结果手术开始后,对照组患者体温下降明显,观察组患者体温基本保持稳定,两组术中各时间点及手术结束、苏醒时体温比较均有统计学意义(P0.001);观察组未发生寒战,对照组6例发生寒战;术后24h观察组C反应蛋白、白介素-6浓度升高幅度低于对照组。结论术中保持患者体温稳定可减少术后寒战及炎症的应激反应。     

蛋白质组学在帕金森病诊断中的研究进展

帕金森病是人类第二大神经退行性疾病,对于其发病机制目前尚不清楚,而运用蛋白质组学方法对该疾病进行研究,有助于对该疾病进行更加深入的了解,对探明其发病机制,寻找可靠的相关生物学标记物,开拓新的治疗方法具有重要意义。笔者主要从两个方面介绍蛋白质组学在帕金森病研究中的应用,一方面是通过获取患者本身的脑部组织和脑脊液进行蛋白研究,另一方面通过制作帕金森病动物模型获得需要的组织进行蛋白研究。