胸部撞击伤后心脏功能变化的实验研究

目的 探讨胸部撞击伤后心脏功能的变化规律。方法 采用兔胸部撞击伤模型，用单光子发射计算机断层照相机（ＳＰＥＣＴ｜在伤前和伤后不同时相点检测心脏功能。结果 右心室射血分数，１／３射血分数，１／３射血率在撞击后２４ｈ仍明显低于撞击前；左心室高峰充盈率，１／３充盈分数和１／３充盈率在撞击后２４ｈ仍明显低于撞击前。     

A Study on Hypotensive Action of Total Saponin of Ziziphus Jujuba Mill and Its Mechanism

Total saponin of Ziziphus Jujuba Mill seeds(ZS) produced amarked hvpotensive effect in anesthetized Sprague-Dawley rats(I. V. or I. P. )and cats (I. V. ). ZS showed no effect on the pressor effect of nor-adrenalinein rats. the depressor effect of ZS was not influenced by atropine nor bydiphenhydramine. ZS markedly inhibited the pressor reflex in catsinduced by ocelusion of the common carotid artery. In rats, ZS inhibitedthe spontaneous discharges of the preganglionic fibers of the greatersplanchnic nerve and decreased the plasma renin activity. These resultsshowed that the hypotensive action of ZS was probably central in origin.     

Extracellular vesicles-associated tRNA-derived fragments (tRFs): biogenesis,biological functions,and their role as potential biomarkers in human diseases

Journal of Molecular Medicine - Traditionally, transfer RNAs (tRNAs) specifically decoded messenger RNA (mRNA) and participated in protein translation. tRNA-derived fragments (tRFs), also known as...     

基于“寒凝血瘀”关键病机理论探讨新加良附方治疗胃癌的网络药理学研究

目的:通过网络药理学方法探讨新加良附方治疗胃癌的作用机制。方法:在中药系统药理学数据库与分析平台(TCMSP)中获取高良姜、香附、穿山龙的有效成分及靶蛋白,通过Genecards、OMIM数据库筛选出胃癌的靶点,利用Venn软件获取共同靶点,使用Cytoscape3.7.2进行"化合物-靶点-疾病"的网络图构建。通过String数据库获取蛋白互作网络(PPI),最后进行GO功能、KEGG通路富集分析。结果:从新加良附方中筛选出高良姜素、槲皮素、木犀草素、薯蓣皂苷元等主要有效活性成分,作用于87个胃癌靶点,筛选出AKT1、MYC、CASP3、EGFR等关键靶点,并确定了PI3K-Akt信号通路、p53信号通路、Wnt信号通路等关键代谢信号通路,参与蛋白结合、转录因子活性、酶活性调节、受体活性等过程。结论:新加良附方通过多个靶点、多条通路发挥治疗胃癌的作用。     

易化PCI治疗对急性心肌梗死患者血浆脑钠肽和左心室重构的影响

目的:探讨易化经皮冠状动脉腔内成形术(PCI)治疗对急性心肌梗死患者血浆脑钠肽水平和左心室重构的影响。方法:选择急性心肌梗死患者212例,其中易化PCI组116例,直接PCI组96例。所有患者分别于入院即刻、24 h、48 h、7 d、14 d及28 d时测定血浆脑钠肽水平;入院后24 h、28 d超声心动图测量左心室舒张末期容积指数、左心室收缩末期容积指数和左心室射血分数。结果:与直接PCI治疗组相比,易化PCI组血浆脑钠肽水平明显降低,左心室射血分数明显升高,左心室客积下降。结论:易化PCI可明显降低血浆脑钠尿肽水平并减轻左心室重构。     

糖皮质激素治疗气管支气管结核的对照研究

目的探讨糖皮质激素（GC）对浸润型及坏死型气管支气管结核的治疗价值。方法 1997年1月至2009年2月住院的浸润型及坏死型气管支气管结核84例,随机分为治疗组（用GC组）和对照组（不用GC组）各42例,治疗组在全身抗结核治疗基础上加用强的松口服6周,对照组常规全身抗结核治疗。观察比较两组的疗效及转归。结果治疗组的有效率97.2%,显著高于对照组81.0%（P〈0.05）。治疗后18个月纤支镜复查,治疗组结核性气管支气管狭窄发生率7.14%,显著低于对照组31.0%（P〈0.01）。结论对炎症浸润型及溃疡坏死型气管支气管结核,早期适量应用糖皮质激素可提高疗效改善预后,减少气管支气管狭窄的发生。     

解偶联蛋白2和缺氧诱导因子1α在肺癌组织中的表达

目的 检测临床肺癌组织中解偶联蛋白2的表达与分布,并分析其与缺氧诱导因子1α在肺癌发生的关系.方法 通过荧光定量PCR方法检测肺癌和癌旁正常组织中解偶联蛋白2与缺氧诱导因子1α的表达,同时应用免疫组织化学、Western blot方法检测解偶联蛋白2在肺癌和癌旁正常组织中的表达.结果 荧光定量PCR方法检测,肺癌组织中解偶联蛋白2与缺氧诱导因子1αmRNA的表达约是癌旁正常组织的3倍,Western blot方法检测,解偶联蛋白2在肺癌组织中的表达量约是癌旁正常组织的2倍;免疫组织化学方法检测,解偶联蛋白2主要在细胞质中表达,在肺癌组织中的表达量明显高于癌旁正常组织,且癌细胞的恶化程度与解偶联蛋白2的含量呈正相关.结论 解偶联蛋白2与缺氧诱导因子1α在人肺癌组织中均有高表达,但两者无明显相关性.     

Ovarian cancer cells with the CD117 phenotype are highly tumorigenic and are related to chemotherapy outcome

Cancer stem cells (CSCs) play an important role in the recurrence and drug resistance of cancer. Isolation and characterization of CSCs from ovarian cancer samples may help to provide novel diagnostic and therapeutic targets in the management of recurrent disease and drug resistance in ovarian cancer. Here, we developed a xenograft model in which cells from 14 samples of human ovarian serous adenocarcinoma tissue or ascites were implanted in immunodeficient mice to test the tumorigenic potential of different populations of ovarian cancer cells. We identified and isolated the tumorigenic cells as CD117⁺Lineage⁻ from three different xenografts. As few as 10³ cells with the CD117⁺Lineage⁻ phenotype, which comprise < 2% of the xenograft tumor cells, were able to regenerate tumors in a mouse model, a 100-fold increase in tumorigenic potential compared to CD117⁻Lineage⁻ cells. The tumors that arose from purified CD117⁺Lineage⁻ cells reproduced the original tumor heterogeneity and could be serially generated, demonstrating the ability to self-renew and to differentiate, two defining properties of stem cells. Furthermore, immunohistochemistry analysis of 25 patients with advanced ovarian serous adenocarcinoma revealed positive immunostaining for CD117 in 40% (10 of 25) of patients. CD117 expression was statistically correlated with resistance to conventional chemotherapy (P = 0.027). In conclusion, our study demonstrates that human ovarian cancer cells with the CD117⁺ phenotype possess the unique properties of CSCs, including self-renewal, differentiation, a high tumorigenic potential, and chemoresistance. Future studies designed to target CD117⁺ cancer cells may identify more attractive and effective therapies for treatment of ovarian cancer.     

浅谈大学药学科研实验室的日常管理

<正>大学中的药学科研实验室不仅肩负培养研究生、本科生等各类药学人才的责任,往往还承担着国家级、省部级等诸多科研课题和研究项目。实现实验室的正常运转与发展壮大,除了要靠加大投入购置各种硬件设施外,还要靠有效的日常管理。实践已经证明,只抓建设不抓管理,则实验仪器、设备配备越多,资源浪费越严重。     

Retiform Sertoli–Leydig cell tumor of ovary in a 9-year-old girl: case report and review of the literature

The management of the retiform variant of Sertoli–Leydig cell tumor remains a challenge for the gynecologist. Surgery is the preferred treatment, but it is still inconclusive whether complete staging or postoperative adjuvant therapy is necessary. A 9-year-old girl was admitted with a well-circumscribed, solid cystic mass in the lower abdomen, of size corresponding to a 20-week gravid uterus, without any androgenic manifestations. Per-operatively, the mass arose from left ovary, which had a smooth outer surface with intact capsule. A cut section was almost multiloculated with cysts ranging from 0.5 to 2.5 cm in diameter and filled with thin yellow or brown serous fluid. Left salpingo-oophorectomy, bilateral lymph node dissection, infracolic omentectomy and appendectomy were performed. The pathological diagnosis was retiform pattern of intermediate to poorly differentiated Sertoli–Leydig cell tumor. The clinical stage was IA. The patient was followed up 3-monthly, and was disease-free at 18-month follow-up after the initial treatment. After review of the literature, we conclude that the retiform variant is a special subtype of Sertoli–Leydig cell tumors. Because of their young age, the uncertain malignant potential and rare bilaterality, patients should be treated conservatively whenever possible. There is at present no good evidence that postoperative adjuvant therapy is effective in preventing recurrence.