家兔呼吸道内分泌细胞的形态学研究

研究新生仔兔和成兔呼吸道内分泌细胞的形态结构及分布，结果显示在呼吸道有单个物成群的内分泌细胞，单个的内分泌细胞从喉直至肺内导气部粘膜上皮内均可观察到，细胞形态多样，细胞胞质充满大量银染黑色颗粒；成群的内分泌细胞，仅分布于肺的支气管树粘膜上皮内，由几个或几十内分泌细胞组成圆形或卵圆形的小体，细胞胞南内也有大一银染黑色颗粒。     

猪全肠外营养模型

白色杂种猪 19只 ,体重 2 2 .3± 2 .4 kg,经皮颈总静脉穿刺前腔静脉置管 ,其中 14只动物完成 2 8d全肠外营养 ( TPN)。 14只动物 ,共施行置管 2 9次 (平均 1.31± 0 .86次 ) ,成功率为10 0 %。再次置管的原因为导管脱出 ( 7/ 2 9)和导管栓塞 ( 2 / 2 9) ,本研究还对与腔静脉置管相关静脉进行了解剖     

人工晶体植入屈光度的术前预选法：眼科超声仪36例对比研究

根据公认的眼科解剖学数据，提出后房型人工晶体植入屈光度的术前预测方法：IOL（D）=1．02（＋21D＋2X）。经人工晶体专用眼科A超36眼实测对比，无显著差异，认为在地市级医院和县级医院后房型人工晶体植入术推广中具有积极意义。     

Monitoring of serum markers for fibrosis during CCl4-induced liver damage. Effects of anti-fibrotic agents.

Liver fibrosis was induced in rats by repeated peritoneal injections of carbon tetrachloride (CCl4) over a period of 2-11 weeks. Serum procollagen III peptide (SPIIINP), prolidase (SP) and alanine aminotransferase (SALT) levels were monitored during the period of induction. The extent of fibrosis was semi-quantitatively estimated after collagen staining, and the anti-fibrotic effects of 16,16-dimethyl prostaglandin E2 (DMPGE2), colchicine, and zinc sulphate were studied. SPIIINP and SP were increased the first 2 weeks after CCl4 administration and peaked at 6 weeks. Alterations in SPIIINP and SP correlated well to the semi-quantitative histological score of liver sections during the first 6 weeks, and SP was positively related to SPIIINP throughout the whole induction period. DMPGE2 decreased SPIIINP, SP and SALT significantly in addition to a markedly decreased formation of liver collagens. Colchicine had a similar but less dramatic effect, whereas zinc sulphate only reduced SPIIINP without influencing liver damage. In conclusion SPIIINP seems to be a valuable indicator of liver fibrogenesis, and SP may play a limited role in indicating accelerated collagen metabolism in the liver. DMPGE2 obviously inhibited the production of collagens induced by CCl4. Colchicine also had an apparent effect on liver fibrosis, whereas zinc sulphate merely seemed to postpone it.     

大黄对实验性急性化脓性胆管炎早期血浆内毒素含量的影响

取ＳＤ大鼠，随机分为三组：正常组、盐水组、大黄组。利用大肠杆菌诱发急性化脓性胆管炎，盐水组和大黄组分别经口灌服生理盐水和大黄１ｍｌ／１００ｇ·日。以血浆内毒素及血清酶学为指标，观察了大黄对大鼠急性化脓性胆管炎早期血浆内毒素及肝功的影响。结果显示：１．盐水组术后３天内死亡４只，大黄组死亡２只，正常组无死亡。２．盐水组大鼠术后３天血浆内毒素含量明显升高，大黄组含量较盐水组明显降低（Ｐ＜０．０１）。３．术后３天血清ＡＫＰ和ＧＰＴ与正常组比均有明显增高，而盐水和大黄组之间无显著性差异（Ｐ＞０．０５）。结果表明大黄具有降低化脓性胆管炎大鼠的早期死亡率及显著降低内毒素血症的作用。     

REGULATING FUNCTION OF COENZYMIZATION AND DECOENZYMIZATION OF THE LACTATE DEHYDROGENASE ISOZYMES IN THE MOUSE TISSUES DURING HYPOXIA

Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH (1 -5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner.Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH,(H4), LDH2(H3M) increased, while RPEA of LDH5(M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4(HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands).     

高职高专院校生物化学教学体会

生物化学这门课程理论性强，内容复杂、抽象、难学难记，教学过程中如果采用灵活多样的教学手段，注意调动学生学习的主动性和积极性，即可克服学生的畏难情绪，从而提高学习效率。     

海正:一条驰逐国际药海的"快鱼"

随着经济全球化的发展和医药科技的日新月异,企业间的竞争不再是“大鱼”吃“小鱼”,而是“快鱼”吃“慢鱼”。在国际医药经济海洋中,谁能最快、最好地研制成功一种新药并成为“第一供应商”,谁就是赢家;谁能最先抢市场制高点,谁就能成为赢家。     

脑干、三叉神经及大脑动脉环的可视化研究

目的 建立人体脑干、三叉神经及大脑动脉环的可视化数字模型。方法 采用首例中国数字化可视人体数据集，在SGI工作站上对脑干及其周围毗邻结构进行计算机三维重建并立体显示。结果 获得人体脑干、三叉神经及大脑动脉环等器官组织的三维重建结构，可对其进行任意径线和角度的适时三维测量。结论 首例中国数字化可视人体数据集能够提供完整而精确的解剖断面数据，脑干及其毗邻的三叉神经、大脑动脉环的可视化模型准确反映出该区域复杂的解剖学结构特点及其相邻器官间的空间毗邻关系，可为该区域的疾病的影像诊断和外科治疗提供形态学依据。