Immunohistochemical identification of tissue factor in solid tumors.

Patients with cancer experience a much higher than expected incidence of thromboembolic disorders, commonly referred as Trousseau syndrome. Although this association has been well documented, the etiology of the hypercoagulable state is not known. The expression on tumor cells of tissue factor (TF), a membrane-bound lipoprotein that functions as a cofactor to factor VIIa in the initiation of the extrinsic pathway of blood coagulation, has been postulated as a possible mechanism. Whereas the distribution of TF in normal tissues is known, no large survey of TF expression in malignant tissues has been reported. In this study a polyclonal, monospecific rabbit anti-human TF IgG was used for immunohistochemical localization of TF antigen in 85 different tumor specimens. In general, cell types which normally express TF continued to do so after malignant transformation (41 of 60 epithelial tumor specimens were positive for TF). Tumors of nonepithelial origin frequently lacked TF, with only 3 of 19 specimens containing evidence of TF antigen. In addition five of six benign tumors did not express TF. Many tumor types commonly associated with Trousseau syndrome, for example lung, pancreatic, breast, colon and gastric carcinomas, stained positively for TF. Based on this survey, it appears that TF expression by tumors may be an important factor in the pathogenesis of a hypercoagulable state in some patients with cancer.     

Does multi-vessel off-pump coronary artery bypass grafting reduce post operative morbidity compared to on-pump CABG?

Background Cardiopulmonary bypass (CPB) may contribute to the complications and it is assumed that eliminating cardiopulmonary bypass has the potential of reducing post operative morbidity after coronary artery bypass grafting (CABG). The study was carried out to compare mortality and morbidity in the off-pump and on-pump CABG groups. Methods We prospectively analysed 200 patients undergoing CABG. Group A consists of 100 patients underwent multi-vessel off-pump CABG and group B consists of 100 patients underwent CABG with CPB. The incidence of complications (mortality, re-exploration for bleeding, myocardial infarction, atrial fibrillation, neurological events, new onset renal failure (s. creatinine>1.6 mg/dL) pulmonary complications, length of ICU stay and hospital stay were recorded, analysed and compared. Results OPCAB patients received 2.73±0.61 grafts/patient and on-pump CABG patients received 3.39±0.75 grafts/patient (p value<0.00001). There was no significant statistical difference in mortality, incidence of stroke between OPCAB and CABG with CPB patients. Length of ICU stay was 32.84±4.22 vs 44.85±7.18 hrs (p value<0.00001) and hospital stay was 6.52±0.69 vs 7.94±0.92 days (p value<0.00001) between group A and group B respectively. Incidence of atrial fibrillation was less in OPCAB group 7% vs 12% although it was statistically not significant (p value 0.33). It was observed in our study that there was no significant deference in worsening of existing renal failure between on-pump CABG and OPCAB 6% vs 2% (P value 0.28). Blood utilization was significantly less in OPCAB group (p value<0.001). Conclusion There was no statistically significant difference in terms of mortality, incidence of stroke and new onset renal failure in both groups. But there was lesser incidence of post operative atrial fibrillation, worsening of existing renal failure in off-pump group though statistically not significant. There was significant reduction in blood utilization, length of ICU and hospital stay in OPCAB group.     

The effects of calcium channel blockers on cyclosporine and its metabolites in renal transplant recipients

Although calcium channel blockers have been reported to increase trough cyclosporine (CsA) blood levels, few studies have systematically examined the effects of calcium channel blockers on CsA pharmacokinetics. In the present investigation, complete pharmacokinetic profiles of CsA and its major metabolites (M1, M17, and M21) were determined in 11 verapamil-treated patients, 7 nifedipine-treated patients, and in 78 controls. Whole blood and urine levels were analyzed using high-performance liquid chromatography. Verapamil caused a 45% increase in CsA area under the curve, maximum concentration, steady-state concentration, and trough level. Metabolite 17 levels were increased in a parallel fashion, suggesting that altered CsA bioavailability rather than decreased metabolism may have caused the higher CsA levels in verapamil-treated patients. However, verapamil-induced reductions in CsA metabolism by other routes could not be ruled out. No changes in CsA or its metabolites were observed in nifedipine-treated patients. Unlike previous reports in patients treated with higher CsA doses, verapamil and nifedipine did not improve renal function in the present study. Nevertheless, the increase in CsA blood levels seen with verapamil may enhance the therapeutic cost-effectiveness of this agent in hypertensive renal transplant recipients.     

Influence of glucose amendment on the toxicity of two nitrophenols to Chlorella vulgaris

Glucose amendment (0.5%) to the culture medium of Chlorella vulgaris reversed the algal toxicity caused by p-nitrophenol (PNP) and m-nitrophenol (MNP), only at their algistatic levels. Algistatic and algicidal concentrations of PNP and MNP, in the absence of glucose, led to the liberation of more nitrite in the culture medium. However, addition of glucose inhibited nitrite formation.     

Hypoglycemic effect of gelatin microspheres with entrapped insulin and protease inhibitor in normal and diabetic rats

Insulin containing gelatin microspheres (IGM) with and without soyabean trypsin inhibitor (TI) were pre-pared and coated with enteric polymers to protect them from degradation in stomach and to release the insulin upon reaching the intestine. Four types of coated IGM were prepared: (i) IGM coated with natural polymers (chitosan inner coat-alginate outer coat), (ii) IGM-TI coated with cellulose acetate phthalate; (iii) IGM-TI coated with cellulose acetate butyrate, and (iv) IGM-TI coated with natural polymers (chitosan inner coat-alginate outer coat). The protective efficiency of uncoated and four types of coated microspheres to-ward digestive enzymes such as pepsin and trypsin was evaluated under simulated physiological conditions. The microspheres were characterized for their insulin content and particle size. The morphology of the micro-spheres was studied using scanning electron micros-copy. The in vitro release studies of insulin from uncoated and coated microspheres indicated that the release followed a zero-order pattern, prolonging for 6 days from 2 days in the case of uncoated spheres. The uncoated and coated microspheres containing insulin (20 IU/kg) were orally administered to albino Wistar rats by stomach tube, and insulin absorption was evaluated by assessing the hypoglycemic effect in normal and diabetic rats. A significant and continuous hypoglycemic effect was observed in diabetic rats following oral administration of coated IGM containing TI when compared to the effect following administration of coated IGM without TI.     

长春西汀片中阿朴长春胺酸乙酯含量的紫外分光光度法测定

采用紫外分光光度法测定长春丁汀片的含量。长春西汀片在２７４ｍｍ波长处有最大吸收，方法线性范围是１０－２０μｇ／ｍｌ，回归方程Ｙ＝３０．０９２Ｘ＋０．０５３３，ｒ＝０．９９９９，平均回收率为１０１．３８％（ｎ＝５，ＲＳＤ＝０．７１％）。