对"特困救助"实施效果的评价

目的：通过对河南省“卫VⅢ”项目县特困救助户和非特困救助户的住院需求、利用及满足程度的比较分析，对特困救助的实施效果进行评价，进而找出特困救助实施过程中存在的问题及解决办法。方法：入户调查收集资料，用FOXPRO软件建立数据库，然后用SAS6.12软件进行统计分析。结果：在同等的住院服务需求下，非救助组的应住院率而未住院率高于特困救助组，在未住院原因构成中，因经济困难而未住院的原因构成非救助组高于救助组。结论：特困救助是一种有效地解决贫困人群就医困难的办法，但尚需不断改革和完善。     

二硫化碳早期损害神经行为检测评价指标研究

目的：研究二硫化碳早期损害神经行为检测敏感指标。方法：采用NES-C3对接触组和对照组进行神经行为检测敏感指标筛选，并以国家规定的有关标准对接触组进行临床验证。结果：提示目标追踪、视觉保留、系列加减等六项指标对二硫化碳早期损害具有较强的敏感性，且与视觉功能的障碍呈明显相关。结论：本研究所建立的六项神经行为检测项目对二硫化碳作业人员进行早期健康损害是一种敏感、有效并且简便的检测评价指标。     

细针吸取活检诊断各种脏器肿块158例总结

1987年7月～1990年6月,我们用细针吸取活检不同脏器肿块病人158例,其中胸腔62例,腹腔37例,体表59例。120例诊断为恶性肿瘤,其中93例明确了组织学类型(77.5%)。经活检或临床观察随访证实其准确率为95%(150/158),假阳性为0.83%(1/120),假阴性18%(7/38)。部分病例同时进行了细胞学和组织学检查,诊断的准确率较高。158例病人均未出现严重并发症。     

变压吸附两步均压制氧试验

根据8M~3/h制氧设备的试验工作,本文评述了变压吸附两步均压制取富氧的方法和设备。试验表明,当切换时间为7秒-9秒-14秒时,与切换时间7秒-7秒-16秒相比,氧浓度可增加1.8％,氧收率可增加0.5％,出氧压力可提高19613.3帕。     

Effect of agonist on gamma-aminobutyric acid B receptor subunit expression in the hippocampus of epileptic rats

BACKGROUND： An amino acid imbalance has been considered to be responsible for epilepsy pathogenesis. Gamma-aminobutyric acid-B receptor （GABABR） inhibits voltage-sensitive calcium ion channels and GABA or glutamic acid （Glu） neurotransmitter release, which promotes or inhibits onset and development of epilepsy. OBJECTIVE： To explore the effect of baclofen on GBRla and GBR2 mRNA expression in the hippocampus of epileptic rats following kainic acid （KA） induction, and to study the adaptability of GABABR subunits. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment based on molecular biology was performed at the Laboratory Research Center of Second Hospital Affiliated to Soochow University from November 2005 to March 2006. MATERIALS： KA was provided by Sigma, USA. In situ hybridization detection kit of GBRla and GBR2 was provided by Wuhan Boster Biological Technology, China. GABABR agonist （baclofen） was provided by Sigma, USA. METHODS： Forty-four epileptic rats were randomly allocated to epileptic （n = 28） and drug intervention （n = 16） groups. The epileptic group was further divided into post-epileptic subgroups at different time points： 6, 12 hours, 1, 3, 7, 15, and 30 days （n = 4）. The drug intervention group was further divided into intervention controls subgroups at various time points： 6 hours, 1 day, and 3 days （n = 4）. Four additional rats were considered the normal control group and not modeled, but were injected with saline in the hippocampal CA3 region. MAIN OUTCOME MEASURES： GBRla and GBR mRNA expression was detected in the right hippocampal CA1, CA3, and dentate gyrus （DG） areas of the control, epileptic, and interference groups at various time intervals according to in situ hybridization results. RESULTS： （1） During the early stage of epilepsy （6 and 12 hours）, GBRla and GBR2 mRNA expression was decreased, and expression was less than the control group at one day after KA induction （P 〈 0.05）. mRNA expression was increased in the DG, but was greater than the control group at day 3 （P 〈 0.05）. Expression in the hippocampal CA1 and CA3 regions remained low （P 〈 0.05）, but gradually recovered to control levels. （2） The time points when subunit expression was decreased were prolonged following baclofen intervention, and expression was significantly greater than the epileptic group （P 〈 0.05）. CONCLUSION： Both mRNA expressions of GABABR subunits were up-regulated following decreased expression in the epileptic group, suggesting that the temporal lobe exhibited endogenous antiepileptic mechanisms during the early stages of epilepsy onset. Baclofen promoted mRNA expression of GBRla and GBR2.     

心肌线粒体及其固定装置在心衰时的形态学改变

研究心衰时心肌细胞线线粒体的立体构筑形态学改变，为心衰机理提供科学依据。方法：用Ｗｉｓｔａｒ大鼠制作后负荷心衰模型；分离心肌细胞；用Ｔｒｔｉｏｎｘ１００去心肌细胞膜和游离蛋白质；制备游离心肌细胞整装骨架，干燥，喷金，日立Ｓ－４５０扫描电镜观察。     

化妆品厂膏霜类灌注室卫生调查

本文通过对本市8家化妆品厂膏霜类化妆品灌注室卫生学调查结果表明;灌注室空气细菌与一般卫生状况呈负相关(r=-0.886),空气细菌与尘埃粒呈正相关(r=0.8829)。因此,要控制膏霜类灌注室因作业环境而引起的一次性污染,在净化空气的同时必须控制尘埃粒,并保持室内良好的卫生状况。     

自身骨髓回输在肿瘤病人造血功能受损时的疗效

本文报道了36例恶性肿瘤患者,大剂量化疗或大面积(全身)放射治疗后,使用冷冻保存(4℃冰箱,干冰,液氮)的自身骨髓对加速造血功能恢复的初步效果。结果表明回输后对受药物或射线抑制的造血和免疫系统有促进恢复和重建之效应。讨论了自身骨髓采取、冷冻保存和活性测定的方法以及骨髓中瘤细胞的分离。自身骨髓输注临床使用安全可靠,对常规治疗无效的患者提供了新的治疗途径。因而对这种方法值得进一步研究。