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排序方式: 共有230条查询结果,搜索用时 15 毫秒
31.
Lutz E Lehmann Stefan Schroeder Wolfgang Hartmann Oliver Dewald Malte Book Stefan U Weber Jens-Christian Schewe Frank Stüber 《European journal of cardio-thoracic surgery》2006,30(1):59-63
OBJECTIVE: Cardiac surgery causes induction and release of inflammatory mediators that may be regulated by genetic background. Macrophage migration inhibitory factor (MIF) is a proinflammatory mediator that is known to be up-regulated in patients undergoing cardiac operations. Here we analyzed genotype distribution and allele frequency of the MIF-173*G/C single nucleotide polymorphism (SNP) and MIF plasma levels in patients undergoing surgical revascularization with (on-pump, n=45) and without (off-pump, n=34) cardiopulmonary bypass (CPB). METHODS: Genotyping was performed using a real-time PCR-based system with a hybridization probe system specific for the MIF-173*G/C SNP. In on-pump patients, blood samples were drawn before start of CPB, after termination of CPB and 12h postoperatively. In off-pump patients, blood samples were collected before stabilizer placement, after removal of the stabilizer and 12h postoperatively. MIF levels were measured using ELISA technique. RESULTS: Genotype distribution and allele frequencies were comparable between on-pump and off-pump patients. When comparing patients according to MIF genotype, a significant increase of MIF plasma levels after completed coronary bypass grafting using CPB was found in patients heterozygous for the MIF-173*G/C SNP (p<0.05). Moreover, on-pump patients showed significantly decreased MIF plasma levels after 12h postoperatively (p<0.05). In off-pump patients, MIF plasma levels were not significantly different over the time-course and were independent of the genotype. CONCLUSIONS: Patients carrying the C-allele showed significantly increased levels of the proinflammatory cytokine MIF compared to G/G homozygous when revascularization was carried out using CPB. The G/C genotype may be associated with a severe inflammatory reaction and therefore preoperative screening could be beneficial for patients undergoing cardiac surgery using CPB. 相似文献
32.
Petersen I Schewe C Schlüns K Dietel M Speich N Schmitt C Bollmann M Sotlar K Bültmann B Dours-Zimmermann MT Padberg B Zimmermann DR 《Virchows Archiv : an international journal of pathology》2007,451(3):701-716
The detection and typing of human papilloma virus (HPV) in pathology specimens is gaining increasingly in importance. In the
context of the initiative for quality assurance in pathology (QuIP) of the German Society of Pathology and the Professional
Association of German Pathologists, four panel laboratories with experience and expertise in polymerase chain reaction (PCR)-based
HPV detection were selected to establish an inter-laboratory trial. In a first step, these laboratories performed an internal
testing of their own methodologies, which comprised DNA sequencing, multiplex nested PCR and hybridization techniques. Material
from 39 samples including paraffin sections and DNA preparations of tissues and plasmids were evaluated by each panel institute
according to their own protocols. Despite the different methodologies, a high degree of inter-laboratory reliability was achieved.
In this report, we summarise the results. Pretested specimens are available for the external trail and can be ordered from
the steering institute via provitro GmbH Berlin (). Supplementary data are online available at (rubric "Forschung"), which includes a web-based photo gallery of HPV-associated lesions and their potential association
with specific virus types. The initiative is intended to foster the quality assurance of molecular HPV analysis in pathology
and its correlation with morphological changes. 相似文献
33.
Successful therapy of hepatitis B with tenofovir in HIV-infected patients failing previous adefovir and lamivudine treatment 总被引:4,自引:0,他引:4
Schildgen O Schewe CK Vogel M Däumer M Kaiser R Weitner L Matz B Rockstroh JK 《AIDS (London, England)》2004,18(17):2325-2327
Three HIV-infected patients with chronic hepatitis B (genotype A) were switched to adefovir therapy after unsuccessful lamivudine treatment. Surprisingly, adefovir therapy failed, although none of the virus isolates displayed mutations known to be associated with adefovir resistance (A181V, N236T). In two isolates we identified hepatitis B virus DNA polymerase mutation L217R, in one case we found multiple frameshifts in the same region. In all cases adefovir was replaced by tenofovir, resulting in a significant drop in the viral load. 相似文献
34.
Brokstad KA Eriksson JC Cox RJ Tynning T Olofsson J Jonsson R Davidsson A 《The Journal of infectious diseases》2002,185(7):878-884
The immune response in the nasal mucosa to influenza vaccination in 23 patients scheduled for tonsillectomy was studied. A statistically significant increase in influenza virus-specific serum and oral fluid antibodies was observed 7 days after vaccination. The numbers of influenza virus-specific antibody-secreting cells (ASCs) in peripheral blood also increased significantly 1 week after vaccination. The numbers of ASCs in tonsils and nasal mucosa were compared with data from a recent study of nonvaccinated volunteers. The numbers of influenza virus-specific ASCs in tonsils were significantly higher in the vaccinated group, but, surprisingly, there was no significant difference between the groups in the numbers of ASCs in nasal mucosa. This suggests that the influenza virus-specific antibodies detected in oral fluid are not produced locally in the nasal mucosa and may originate from a systemic source, indicating that the vaccination may favor a systemic immune response. 相似文献
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38.
Dr. jur. Dr. med. Günter Schewe 《International journal of legal medicine》1968,62(2):124-129
Zusammenfassung Die unterschiedlichen Bedeutungen des Willensbegriffs und die sich daraus ergebenden psychologischen, psychiatrischen und rechtsdogmatischen Fragen werden diskutiert.
Summary The different meanings of the conception of purposely action and the resulting problems in psychology, psychiatry and penal law are discussed.相似文献
39.
40.
Fleischhacker M Schulz S J?hrens K von Lilienfeld-Toal M Held T Fietze E Schewe C Petersen I Ruhnke M 《Clinical microbiology and infection》2012,18(10):1010-1016
Hepatic Candida infection (HCI; known as chronic disseminated candidosis or CDC) is a distinct form of disseminated Candida infection with predominant involvement of the liver. Diagnosis of HCI is usually made on clinical suspicion together with multiple lesions in liver on ultrasound (US), CT and/or MRI scan. Fungal elements may not always be visible in liver tissue and mycological culture is frequently negative, making the evidence for proven fungal disease difficult. We studied a novel commercially available low-cost and density-array (LCD) chip technique for a molecular diagnosis of HCI. This is a two-step procedure with PCR amplification after DNA extraction followed by hybridization on a small chip provided by the manufacturer (Fungi 2.1, Chipron GmbH). The analysis of DNA from 45 fungal control strains showed an excellent specificity and sensitivity. The DNA from 11 liver biopsies of patients with haematological malignancies suffering from CDC was analysed on the LCD chip and overall 11 fungal pathogens could be detected in eight liver biopsies, supporting the clinical diagnosis of HCI/CDC. Analysis of liver biopsies from controls was negative for fungal DNA in all samples studied. In conclusion, the novel LCD chip technique examined in our study was able to detect fungal pathogens in liver biopsies from patients with haematological malignancies and suspected HCI/CDC but was negative in control biopsies. 相似文献