烧伤创面渗液预处理的单核-巨噬细胞对表皮细胞增殖率的影响

目的观察深Ⅱ度烧伤创面巨噬细胞对表皮细胞增殖的作用。方法用创面渗液刺激分离自血液的单核－巨噬细胞,取其培养上清液刺激表皮细胞,ＭＴＴ法检测表皮细胞的增殖率。用等比例稀释的渗液作对照。结果其培养液均能促进表皮细胞增殖,但晚期创面渗液的作用强度较低。结论用烧伤创面渗液激活的单核－巨噬细胞可作为创面巨噬细胞的替代品;创面巨噬细胞对表皮细胞具有促进增殖的作用,晚期创面中稍弱。     

Influence of intense light stimulation on trigeminal and cervical pain perception thresholds

Thirty-three migraineurs and 23 healthy controls were submitted to pressure algometry before and after light-induced discomfort was elicited by progressive light stimulation in a monoblind fashion. Pressure algometries were performed on the emergence of the supraorbital, infraorbital, mental and greater occipital nerves, and over the temporal muscles, always throughout the same sequence and from right to left. Measurements were carried out before and immediately after light stimulation and after 10 min of the second algometry. The final result for each site measured at each time-point was the mean of the three measurements. Light stimulation was carried out progressively until light-induced discomfort was reported, to a maximum of 20,000 lux. A heat-blocking glass protected patients' eyes. Migraineurs presented significant and persistent drops in pain perception thresholds after light stimulation, at all sites tested (P = 0.002 to < 0.0001). These drops were not seen in controls, in whom, conversely, a less significant increase was seen on right infraorbital and left temporal muscle sites. Our results indicate that in migraineurs, light may have a relevant role in trigeminal and cervical pain perception thresholds.