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排序方式: 共有2735条查询结果,搜索用时 15 毫秒
41.
Lydie Canier Nimol Khim Saorin Kim Rotha Eam Chanra Khean Kaknika Loch Malen Ken Pieter Pannus Philippe Bosman Jorgen Stassijns Fabienne Nackers SweetC Alipon Meng Chuor Char Nguon Chea William Etienne Martin De Smet Jean-Marie Kindermans Didier Ménard 《The American journal of tropical medicine and hygiene》2015,92(3):573-577
In the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas. 相似文献
42.
Xavier Henry Oliver Verlaine Ana Amoroso Jacques Coyette Jean-Marie Frère Bernard Joris 《Antimicrobial agents and chemotherapy》2013,57(12):6358-6360
The opportunistic human pathogen Enterococcus faecium overproduces the low-affinity PBP5. In clinical strains, mutations in PBP5 further reduce its acylation rate by β-lactams. Previous studies have reported that ceftaroline had poor inhibitory activity against β-lactam-resistant E. faecium strains. In this study, we show that ceftaroline exhibits killing activity against our laboratory-derived ampicillin-resistant E. faecium mutant that overproduces a wild-type PBP5 and that ceftaroline inactivates PBP5 much faster than benzylpenicillin and faster than ceftobiprole. 相似文献
43.
Laetitia Travier Olaya Rendueles Lionel Ferrières Jean-Marie Herry Jean-Marc Ghigo 《Antimicrobial agents and chemotherapy》2013,57(8):3960-3968
Antivirulence strategies targeting bacterial behavior, such as adhesion and biofilm formation, are expected to exert low selective pressure and have been proposed as alternatives to biocidal antibiotic treatments to avoid the rapid occurrence of bacterial resistance. Here, we tested this hypothesis using group 2 capsule polysaccharide (G2cps), a polysaccharidic molecule previously shown to impair bacterium-surface interactions, and we investigated the nature of bacterial resistance to a nonbiocidal antibiofilm strategy. We screened an Escherichia coli mutant library for an increased ability to form biofilm in the presence of G2cps, and we identified several mutants displaying partial but not total resistance to this antibiofilm polysaccharide. Our genetic analysis showed that partial resistance to G2cps results from multiple unrelated mutations leading to modifications in surface physicochemical properties that counteract the changes in ionic charge and Lewis base properties induced by G2cps. Moreover, some of the identified mutants harboring improved biofilm formation in the presence of G2cps were also partially resistant to other antibiofilm molecules. This study therefore shows that alterations of bacterial surface properties mediate only partial resistance to G2cps. It also experimentally validates the potential value of nonbiocidal antibiofilm strategies, since full resistance to antibiofilm compounds is rare and potentially unlikely to arise in clinical settings. 相似文献
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Florence Pilate Zhi-Bin Wen Farid Khelifa Yan Hui Sebastien Delpierre Luo Dan Rosica Mincheva Philippe Dubois Ke-Ke Yang Jean-Marie Raquez 《RSC advances》2018,8(48):27119
A novel poly(epsilon-caprolactone) (PCL) supramolecular network exhibiting shape-memory behavior was successfully constructed with pendant UPy units that are highly able to dimerize. The dynamic network was obtained by a simple and versatile strategy consisting of chain-extension reaction between α,ω-dihydroxyoligoPCL and hydroxylated UPy units in the presence of hexamethylene diisocyanate as a coupling agent and further intermolecular dimerization of the UPy along the polyurethane backbone. 1H NMR analyses confirmed the dynamic features of the system, and DMTA in tensile mode was investigated to assess the SMP properties. Recyclability was also assessed by taking advantage of these supramolecular networks. Further addition of cellulose nanocrystals into the polymer network enabled adjustment of the extent of the net-points and therefore the SMP features. As confirmed by dispersion tests in solution and SEM observations, these bio-based nanofillers were homogeneously distributed in the network via supramolecular interaction between the hydroxyl groups present on their surface and UPy moieties along the polyurethane backbone. Thus, the here developed nanomaterials might reveal applicability in areas where a combination of SMP and biocompatibility is needed.Novel melt-recyclable poly(ε-caprolactone)/cellulose nanocrystals supramolecular nanocomposite networks with shape-memory behavior have been successfully constructed by playing with UPy chemistry. 相似文献
47.
New insights on IgA vasculitis with underlying solid tumor: a nationwide French study of 30 patients
Hankard Antoine Michot Jean-Marie Terrier Benjamin Brihaye Benoît Chanal Johan Combe Christian Karras Alexandre Urbanski Geoffrey Amoura Zahir Darrigade Anne-Sophie Deroux Alban Guerville Florent Burel Le Sébastien Maigné Gwénola Mekinian Arsène Moulis Guillaume Riviere Etienne Vandamme-Giard Carole Maillot Francois De Boysson Hubert Aouba Achille Audemard-Verger Alexandra 《Clinical rheumatology》2021,40(5):1933-1940
Clinical Rheumatology - IgA vasculitis (IgAV) frequently occurs during or after a mucosal infection; it also rarely occurs in patients with cancer. We hypothesized that cancer could impact the... 相似文献
48.
Nosheen Fatima Rana Nicolas Sauvageot Jean-Marie Laplace YinYin Bao Ingolf Nes Alain Rincé Brunella Posteraro Maurizio Sanguinetti Axel Hartke 《Infection and immunity》2013,81(8):2662-2668
Enterococcus faecalis is a highly stress resistant opportunistic pathogen. The intrinsic ruggedness of this bacterium is supposed to be the basis of its capacity to colonize the hostile environments of hospitals and to cause several kinds of infections. We show in this work that general resistance to very different environmental stresses depends on the ability of E. faecalis to maintain redox balance via lactate dehydrogenase (LDH). Furthermore, LDH-deficient mutants are less successful than the wild type at colonizing host organs in a murine model of systemic infection. Taken together, our results, as well as those previously published for Staphylococcus aureus (A. R. Richardson, S. J. Libby, and F. C. Fang, Science 319:1672–1676, 2008), identify LDH as an attractive drug target. These drugs may have additional applications, as in the fight against glycopeptide antibiotic-resistant bacteria and even cancer. 相似文献
49.
Harry Sokol Sophie Georgin-Lavialle Danielle Canioni Stéphane Barete Gandhi Damaj Erinn Soucie Julie Bruneau Marie-Olivia Chandesris Felipe Suarez Jean-Marie Launay Achille Aouba Catherine Grandpeix-Guyodo Fanny Lanternier Bernard Grosbois Christian de Gennes Pascal Cathébras Olivier Fain Nadia Hoyeau-Idrissi Patrice Dubreuil Olivier Lortholary Laurent Beaugerie Brigitte Ranque Olivier Hermine 《The Journal of allergy and clinical immunology》2013
50.
Ravel Jean-Marie Benkirane Mehdi Calmels Nadège Marelli Cecilia Ory-Magne Fabienne Ewenczyk Claire Halleb Yosra Tison François Lecocq Claire Pische Guillaume Casenave Philippe Chaussenot Annabelle Frismand Solène Tyvaert Louise Larrieu Lise Pointaux Morgane Drouot Nathalie Bossenmeyer-Pourié Carine Oussalah Abderrahim Guéant Jean-Louis Leheup Bruno Bonnet Céline Anheim Mathieu Tranchant Christine Lambert Laëtitia Chelly Jamel Koenig Michel Renaud Mathilde 《Journal of neurology》2021,268(5):1927-1937
Journal of Neurology - STUB1 has been first associated with autosomal recessive (SCAR16, MIM# 615768) and later with dominant forms of ataxia (SCA48, MIM# 618093). Pathogenic variations in STUB1... 相似文献