全文获取类型
收费全文 | 1053篇 |
免费 | 70篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 42篇 |
妇产科学 | 8篇 |
基础医学 | 167篇 |
口腔科学 | 25篇 |
临床医学 | 102篇 |
内科学 | 233篇 |
皮肤病学 | 15篇 |
神经病学 | 72篇 |
特种医学 | 20篇 |
外科学 | 180篇 |
综合类 | 33篇 |
预防医学 | 71篇 |
眼科学 | 22篇 |
药学 | 67篇 |
中国医学 | 15篇 |
肿瘤学 | 49篇 |
出版年
2024年 | 4篇 |
2023年 | 12篇 |
2022年 | 38篇 |
2021年 | 66篇 |
2020年 | 26篇 |
2019年 | 40篇 |
2018年 | 41篇 |
2017年 | 30篇 |
2016年 | 42篇 |
2015年 | 32篇 |
2014年 | 45篇 |
2013年 | 74篇 |
2012年 | 53篇 |
2011年 | 74篇 |
2010年 | 49篇 |
2009年 | 30篇 |
2008年 | 73篇 |
2007年 | 62篇 |
2006年 | 65篇 |
2005年 | 55篇 |
2004年 | 68篇 |
2003年 | 48篇 |
2002年 | 36篇 |
2001年 | 15篇 |
2000年 | 11篇 |
1999年 | 7篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有1127条查询结果,搜索用时 31 毫秒
111.
Gupta R Zaidat O Majid A 《Stroke; a journal of cerebral circulation》2007,38(9):e80; author reply e81-e80; author reply e82
112.
Liu J Desai A Onuchic JN Hwa T 《Proceedings of the National Academy of Sciences of the United States of America》2007,104(41):16104-16109
During mitosis, the condensed chromosomes undergo a series of spectacular oscillations after they are captured in an end-on manner by kinetochore microtubules (KMT) emanating from the spindle poles. Such oscillations are commonly attributed to tug-of-war-like mechanisms, where the mechanical force imbalance alone drives the chromosome movement. However, a large portion of the force imbalance upon the chromosome is absorbed by the kinetochore and may not drive chromosome movement directly. Mounting evidence suggests that such resistance by the kinetochores regulates the chemical reactions of KMT plus-end growth and shrinkage, which have been shown as the determinant of the chromosome antipoleward (AP) and poleward movements. Here we incorporate this important regulatory feature, propose a mechanobiochemical feedback mechanism, and apply it to the monooriented chromosome oscillation, the early stage of the series of observed chromosome oscillations. In this model, the mechanical movement of the chromosome and the local biochemical reactions at the attached kinetochore region form a feedback loop that drives the oscillation. The force imbalance exerted on the chromosomes provides a bias (via mechanically sensitive proteins) on the local biochemical reactions controlling the KMT plus-end dynamics, and the movement of the chromosome in turn changes the forces exerted on it through the experimentally supported gradient in AP force. The proposed feedback mechanism can generate oscillatory behavior that depends on the topology of the feedback loop but is largely independent of the detailed molecular mechanism. We suggest potential molecular players, whose perturbation may allow direct experimental tests of the model. 相似文献
113.
114.
Introduction
Status epilepticus (SE) is a medical emergency requiring prompt treatment to try to limit mortality and improve outcome. So far, newer antiepileptic drugs (AED) have not assumed a noticeable role in the treatment of SE. This may be in part due to the lack of IV forms for the newer AEDs. The IV form of Levetiracetam (IV-LEV) has recently become available and has a potential role in the treatment of SE. 相似文献115.
The hydrolethalus syndrome protein HYLS-1 links core centriole structure to cilia formation 下载免费PDF全文
Alexander Dammermann Hayley Pemble Brian J. Mitchell Ian McLeod John R. Yates III Chris Kintner Arshad B. Desai Karen Oegema 《Genes & development》2009,23(17):2046-2059
Centrioles are subcellular organelles composed of a ninefold symmetric microtubule array that perform two important functions: (1) They build centrosomes that organize the microtubule cytoskeleton, and (2) they template cilia, microtubule-based projections with sensory and motile functions. We identified HYLS-1, a widely conserved protein, based on its direct interaction with the core centriolar protein SAS-4. HYLS-1 localization to centrioles requires SAS-4 and, like SAS-4, HYLS-1 is stably incorporated into the outer centriole wall. Unlike SAS-4, HYLS-1 is dispensable for centriole assembly and centrosome function in cell division. Instead, HYLS-1 plays an essential role in cilia formation that is conserved between Caenorhabditis elegans and vertebrates. A single amino acid change in human HYLS1 leads to a perinatal lethal disorder termed hydrolethalus syndrome, and we show that this mutation impairs HYLS-1 function in ciliogenesis. HYLS-1 is required for the apical targeting/anchoring of centrioles at the plasma membrane but not for the intraflagellar transport-dependent extension of the ciliary axoneme. These findings classify hydrolethalus syndrome as a severe human ciliopathy and shed light on the dual functionality of centrioles, defining the first stably incorporated centriolar protein that is not required for centriole assembly but instead confers on centrioles the capacity to initiate ciliogenesis. 相似文献
116.
117.
118.
119.
120.