Effect of injury to the zone of the medial forebrain bundle and preoptic region on activity of an epileptiform focus induced by penicillin (on the phenomenon of the hyperactive determinant dispatch station)

Experiments on cats showed that injury to the medial forebrain bundle (MFB) and also partly to the preoptic region on the side of application of penicillin to the cerebral cortex (middle suprasylvian gyrus) causes depression of paroxysmal activity (spike potentials) in the penicillin focus, and also in a secondary mirror focus arising in the symmetrical zone of the opposite cortex. Injury to MFB on the side of the mirror focus causes depression of paroxysmal spike potentials only in that focus and does not affect activity in the primary epileptiform focus. The effects described are examined from the standpoint of views regarding the role of the determinant dispatch station (DDS) in the activity of the CNS: A primary epileptiform focus is a hyperactive DDS which induces the appearance of secondary foci, supports them, and determines the character of their activity. The results of the investigation suggests a role for MFB in the modulation of cortical epileptiform activity.Laboratory of General Pathology of the Nervous System, Institute of General Pathology and Pathophysiology, Academy of Medical Sciences of the USSR, Moscow. Laboratory of Electrophysiology, V. F. Filatov Odessa Research Institute for Eye Diseases and Tissue Therapy, Ministry of Health of the Ukrainian SSR. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 82, No. 12, pp. 1413–1416, December, 1976.     

Effect of avitaminosis B6 in mice on T-lymphocyte function in vitro

The effect of different degrees of avitaminosis B₆ in mice on the cytolytic activity of T lymphocytes, measured as the quantity of Na₂Cr⁵¹O₄ released from lysed target cells, was studied on a model of the primary immune response in a mixed lymphocyte culture in vitro. Keeping animals for 3 weeks on a diet without pyridoxine did not affect the ability of the lymphocytes to proliferate in vitro or their cytolytic activity. In animals receiving a diet without pyridoxine for 45 days the content of pyridoxal-5-phosphate in the spleen was 55% lower than in the control. Lymphocytes taken from these animals, when cultured in vitro, showed sharply weakened ability to incorporate [³H]thymidine into DNA in response to the alloantigen. The cytolytic activity of these lymphocytes also was reduced. The ability of different forms of pyridoxine to restore the functions of T lymphocytes, when disturbed by avitaminosis B₆, was studied.Laboratory of Systemic Blood Diseases, Oncologic Scientific Center, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR N. A. Kraevskii.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 8, pp. 185–188, August, 1977.     

Nociceptive muscle reflex responses in human forearms

In healthy volunteers, 5-min noxious stimulation with rectangular electrical pulses applied transcutaneously to the phalanges enhanced tonic activity in the palmar and finger flexor muscles resulting in a specific electromyographic pattern consisting of two successive bursts of activity which appeared after a period of inhibition. In patients with chronic pain in the arm, significantly lower thresholds for the first and the second waves of reflex activity have been found. This electromyographic pattern of the forearm muscle reflex responses is supposed to be similar to the nociceptive flexor reflex in the leg. It can be useful for objective assessment of the effectiveness of analgesia and pain syndrome therapy in patients with cervical spinal cord injury. Translated fromByulleten' Eksperimental'noi Biologii I Meditsiny, Vol. 126, No. 9, pp. 278–282, September, 1998     

用点突变的方法组建巨细胞病毒抗原决定簇基因的表达克隆

研究资料表明，人巨细胞病毒（ＨＣＭｖ）单一蛋白的单一抗原决定簇只能被部分患者阳性血清识别。组建在血清学诊断中能够替代全病毒抗原的基因工程抗原，需要含有病毒多种主要抗原蛋白的抗原决定簇。为搞清在表达载体中重复插入某一抗原决定簇基因是否能表达出更高抗原效价的融会蛋白，我们用点突变的方法，在表达载体中分别插入了人ＨＣＭｖ的ｐｐＵＬ３２蛋白羧基端一个抗原决定簇基因的１个、２个和３个拷贝。在免疫转印检测中，这些克隆表达的融合蛋白与特异性阳性血清的反应性差别不明显。这表明，插入表达载体中目的基因的多寡对表达蛋白的抗原效价没有显著影响。     

Investigation of the morphology of cell clones, derived from the mammalian EBTr cell line and their susceptibility to vaccine avian poxvirus strains FK and Dessau

The ability for replication of vaccine avian pox viral strains FK and Dessau in cell clones, derived from the EBTr cell line, derived from embryonic bovine trachea, was studied. The derived seven cell clones showed different morphological characteristics and diverse sensitivity to both vaccine avian pox viral strains. Hence, the EBTr-derived cell clones could be used for cultivation, as well as for differentiation of vaccine avian pox viral strains. In addition, studies have been undertaken to elucidate the possible use of cultivated strains in these heterologous cell culture system's vaccine avian pox viral strains for biotechnology, as well as for solving problems, related to infection of people with avian viruses.     

Partial characterization of a Hantavirus isolated from a Clethrionomys glareolus captured in Belgium

A Hantavirus was isolated in Vero-E6 cells from lungs of a free living bank vole (Clethrionomys glareolus) captured in Turnhout, Province of Antwerp--Northern part of Belgium. With help of monoclonal antibodies the Belgian Hantavirus isolate could be clearly differentiated from Hantaan virus strain 76-118, Prospect Hill virus strain PH1 and SR11, a Hantavirus isolated from laboratory Wistar rat in Japan, but not from the nephropathia epidemica virus strain H?lln?s.